CAZyme Information: MGYG000004337_01195

Basic Information

• Species: Phascolarctobacterium sp000436095
• Lineage: Bacteria; Firmicutes_C; Negativicutes; Acidaminococcales; Acidaminococcaceae; Phascolarctobacterium; Phascolarctobacterium sp000436095
• CAZyme ID: MGYG000004337_01195
• CAZy Family: GT83
• CAZyme Description: Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
516	MGYG000004337_8|CGC1	58725.53	6.8399

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004337	1880847	MAG	Israel	Asia

• Gene Location: Start: 41133; End: 42683 Strand: -

Full Sequence      

MKNLNLQSLIIIAVLALVTILWGIGSVPLFDPDEPVYAETAREMIKYNDYLSPRIFDNFW
YDKPPMYYWLVAASIGLFGDGEAAARFPAALMAVATSVMLYFSASNIFSERAGFWASIVL
TTCVEFFYLGKAAVTDTTLLFFMTAALLCYQHKWYWVMYIAMALATVTKGPIGIVFPGAI
IFLHLLFTGNLREILKMHVIRGLIVYFAIAAPWYYLMYQAHGMEFINTFLGFHNLTRFTT
PEHPDRVLWWYYLPVIILGMFPWTGLLLQSILASISEASSEELVKMSFFNIWWVFVLLFF
SISQTKLVSYILPMFPALAIIIGWNLARLEKQRGESLLSWVIGTVIMFGLLGAGWLIGGN
QLPEAFLEASVLSGTTFVVGLVIIWFLWRERDVIFAGYLHAAMGFLTMLIAFSFILPPVA
DRFSVKTMAAEFVENCDISKPLYVDKFLRPGFMYYTGIAGEEMLPKTDALQKAVSDNTPK
YVVVRRLEFKRLQNPEAIQNLRIVKEKDGIMILEKY

Enzyme Prediction     

No EC number prediction in MGYG000004337_01195.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT83	3	430	5.4e-117	0.8166666666666667

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1807	ArnT	2.60e-59	8	396	9	408	4-amino-4-deoxy-L-arabinose transferase or related glycosyltransferase of PMT family [Cell wall/membrane/envelope biogenesis].
PRK13279	arnT	1.49e-32	9	352	8	359	lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase.
pfam13231	PMT_2	1.74e-20	62	215	1	159	Dolichyl-phosphate-mannose-protein mannosyltransferase. This family contains members that are not captured by pfam02366.
pfam02366	PMT	1.41e-08	10	226	1	245	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QTV77242.1	1.41e-225	1	515	1	518
AOH48432.1	7.75e-168	2	514	5	524
QNH53437.1	2.20e-167	2	514	5	524
ANR70824.1	5.78e-165	2	514	5	524
AME03144.1	1.91e-155	2	512	5	522

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5EZM_A	2.05e-34	29	381	54	421	CrystalStructure of ArnT from Cupriavidus metallidurans in the apo state [Cupriavidus metallidurans CH34],5F15_A Crystal Structure of ArnT from Cupriavidus metallidurans bound to Undecaprenyl phosphate [Cupriavidus metallidurans CH34]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O67270	3.79e-32	6	351	2	346	Uncharacterized protein aq_1220 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=aq_1220 PE=3 SV=1
O67601	9.80e-31	7	350	3	330	Uncharacterized protein aq_1704 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=aq_1704 PE=3 SV=1
Q6D2F3	2.86e-29	5	352	7	362	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) OX=218491 GN=arnT PE=3 SV=1
C6DAW3	7.05e-29	5	380	7	395	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Pectobacterium carotovorum subsp. carotovorum (strain PC1) OX=561230 GN=arnT PE=3 SV=1
A0KGY4	1.20e-28	28	351	24	355	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049) OX=380703 GN=arnT PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.998050	0.000462	0.000126	0.000003	0.000002	0.001367

TMHMM  Annotations     

start	end
7	29
83	105
112	134
154	187
199	221
248	270
283	302
307	329
336	358
365	387
394	416