CAZyme Information: MGYG000004314_01389

Basic Information

• Species: Varibaculum timonense
• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Actinomycetales; Actinomycetaceae; Varibaculum; Varibaculum timonense
• CAZyme ID: MGYG000004314_01389
• CAZy Family: GH110
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1304	MGYG000004314_13|CGC1	141207.63	9.4618

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004314	2027678	MAG	Israel	Asia

• Gene Location: Start: 16032; End: 19946 Strand: +

Full Sequence      

MLHTVFARLNPASHLCPPEKPKTLNKIISVIGILSLVITALALTAPTTALAKEKESITVN
ATAFGADPSGKTDSAPAVEKAIEKLKELRKAHPDKSIVLRFPTGRYDFYPDKAPECTLYV
SNTVGASERFKKKRIGILIEDLDDVTIDGSGSLFMYHGKMTEFAAINSKKIKIQNVATDF
ATPTVIDIAVEKVDSDTKTATFLVPKQYNYSLNGNDITWYSDSSPYSNQRYWTTKQSEFS
YMQAYDASKQQTWRAGNPNSTIFANVTSIKEIKTDNASHLKVTYSSPLNKLLKKGMYFQM
RLLDRDHPGMFIWKSKDVAIEKTQIGFLHGFGIVGQHSENITIDNVRFEAPAETGRSTSG
FADFIQMSGTKGKIQIINSYFSNPHDDPINVHGTFNEVVEKIAANKIRVRFKHHETAGFP
NYFIGDQVEFSTKGNMITVPDSVRTVTAVDGPDGRGGIQGEGSGNLTDIILTFDKDIPAQ
IGVNGTHVVENITYTPEVEISNNVFTATPTRGILVTTRKKVVIENNTFDGIGMAGIYISN
DAQGWYESGPVRNLTIKNNTFLNGSSQAIFIQPTNPTVSETNTVHSNVTITGNKFYILRA
GAQNPAGNGRDAQRVLDAKSTKDLSFTDNTIWLQDPGLLTLTSEQKIVPVGGSMRLLTSG
SPMTNTSLYRLQGNKTVTIANNKYGQGLDTSVDVSQMDSANINISNDLAKTNGAVVTNPS
TSPTVTRYFVSSNPNVIKVDSEGIVYALSEGSATLTMTEVVGTGDKAVKRTSNPLTLQVS
TTAGVPQEIYSFTDKWSIETEDSAHQIKPKEFGFTSTYSSGGLYQEQQGKNILVGQVIAP
ESSSDFEITVRQQGLTSSSWSDTGVYLYKDQNNYLAIQRKHRNGNIRKIAQVRESSAKAQ
ETWYGGNETVRAGENAESVLLKFSKQGNTVTTYFSADNGKSWNKIGQADASFLGKDIKLA
FASASSSDPDGNNTPVSYTDITLNGKKIKLGTKKTVQDITPSGKIPTAKNTQARITGSVS
GLLDGEITTIPNVFNYAATTDAAHSVINAAFKTSPKATMRLQLNNKELKPGADGTINSIQ
LAPGTNILKADVIAEDGITHSVYRWTMLNTDTHANSTPATNEASRSVQVRVPNDQANMGT
VAIEPNTIKGAKLYKGDTVTLKATPAEGYRFTGWRLADKTVSFEPEFSYTVTDRDVIFNA
TFAPIASPDTPPIDTPPSDTPTTNPPTTGGATPDTSSGGKATSKGVASQKGTTRQAQSGK
NSKPTVAGLTKTGSQYVFLLALTSFILVSGGAIIRKPKRSQLRK

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH110	59	633	2.6e-169	0.9470802919708029

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam13229	Beta_helix	5.06e-09	497	596	10	111	Right handed beta helix region. This region contains a parallel beta helix region that shares some similarity with Pectate lyases.
pfam13229	Beta_helix	1.41e-08	492	596	51	140	Right handed beta helix region. This region contains a parallel beta helix region that shares some similarity with Pectate lyases.
pfam05048	NosD	1.68e-06	482	594	62	153	Periplasmic copper-binding protein (NosD). NosD is a periplasmic protein which is thought to insert copper into the exported reductase apoenzyme (NosZ). This region forms a parallel beta helix domain.
pfam18998	Flg_new_2	2.94e-06	1156	1206	22	74	Divergent InlB B-repeat domain. This family of domains are found in bacterial cell surface proteins. They are often found in tandem array. This domain is closely related to pfam09479.
pfam13229	Beta_helix	1.99e-05	491	588	73	157	Right handed beta helix region. This region contains a parallel beta helix region that shares some similarity with Pectate lyases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBA55548.1	2.32e-226	64	762	29	708
AFL04872.1	1.27e-224	64	762	29	708
QRI58269.1	1.45e-224	64	762	34	713
VEG17902.1	1.68e-224	32	762	18	731
BAQ98494.1	1.78e-224	64	762	29	708

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7JW4_A	4.70e-38	64	631	33	566	Crystalstructure of PdGH110B in complex with D-galactose [Pseudoalteromonas distincta],7JW4_B Crystal structure of PdGH110B in complex with D-galactose [Pseudoalteromonas distincta]
7JWF_A	2.00e-37	64	631	33	566	Crystalstructure of PdGH110B D344N in complex with alpha-(1,3)-galactobiose [Pseudoalteromonas distincta],7JWF_B Crystal structure of PdGH110B D344N in complex with alpha-(1,3)-galactobiose [Pseudoalteromonas distincta],7JWF_C Crystal structure of PdGH110B D344N in complex with alpha-(1,3)-galactobiose [Pseudoalteromonas distincta],7JWF_D Crystal structure of PdGH110B D344N in complex with alpha-(1,3)-galactobiose [Pseudoalteromonas distincta]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
B1V8K7	5.00e-153	59	781	35	725	Alpha-1,3-galactosidase A OS=Streptacidiphilus griseoplanus OX=66896 GN=glaA PE=1 SV=1
Q826C5	2.17e-147	57	692	39	622	Alpha-1,3-galactosidase A OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=glaA PE=3 SV=1
Q64MU6	2.84e-85	49	631	16	550	Alpha-1,3-galactosidase A OS=Bacteroides fragilis (strain YCH46) OX=295405 GN=glaA PE=3 SV=1
Q5L7M8	9.99e-85	49	631	16	550	Alpha-1,3-galactosidase A OS=Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / CCUG 4856 / JCM 11019 / NCTC 9343 / Onslow) OX=272559 GN=glaA PE=3 SV=1
Q8A2Z5	4.79e-81	76	597	22	509	Alpha-1,3-galactosidase A OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=glaA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.034294	0.962067	0.002593	0.000449	0.000292	0.000297

TMHMM  Annotations     

start	end
27	49
1272	1294