CAZyme Information: MGYG000004230_02274

Basic Information

• Species: Clostridium sp900759995
• Lineage: Bacteria; Firmicutes_A; Clostridia; Clostridiales; Clostridiaceae; Clostridium; Clostridium sp900759995
• CAZyme ID: MGYG000004230_02274
• CAZy Family: GH95
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1913	MGYG000004230_63|CGC1	213259.78	4.7121

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004230	3516057	MAG	United Kingdom	Europe

• Gene Location: Start: 6337; End: 12078 Strand: -

Full Sequence      

MIKKKKIISVMLVVTMLLSIDRAIFALENKVEDIKTINNKDLRLWYESPAEDSYNGWEKW
SLPLGNGYMGASVFGGVEYERIQLTEKTFWSGGPSPSRPNYNGGNIVTNAKVKVNGVDVP
IMKAIQDAFARGDTATANSLCNKLTGVSDDAGTKGYGYFLSYGNMYLDFKNGVKTNNIQH
YVRDLDLNTAISSVKYDYNNVNYTREYFISYPDNVMVTKLSASENKKLSFDVRVEPDNTG
GGGPNTNNGYSRRFTRTSKDGVITIAGELNDNEMKFNSQTKVINTGGIITDLADGKVNVT
NADEVIIITSIATDYKNDYPNYRTGETDAEVANKVSKRVTDAAKKGYEGLKESHLADYQS
IFSRLNLDLGQTASTVSTDKLLAKYNNGTAPKSEERALEVMLFQYGRYLSIASSREGSLP
SNLQGIWVGGNNSPWHSDYHMNVNLQMNYWPSYSTNMTETALPLIEYIDSLREPGRVTAE
IYANIKSTDENKENGFMAHTQNTPFGWTCPGWNFDWGWSPAAVPWILQNVWEYYEYTGDL
EFMRTKIYPMLKEEAVLYEQMLIEDPETGKLVCSPAYSPEHGPRTNGNTYEQSLIWQMYE
DVITAAKLVGEDSEKILKWENIKNNLKGPIEIGDNGQIKEWYEETTLGSMGQRGHRHMSH
LLGLFPGDLISLETPELLKSAIISLNDRGDQSTGWAMGQKINTWARTGDGNRAYKLIRTL
FKSGILTNLWDTHSPYQIDGNFGMTSGVAEMLMQSNLGYINLMAAMPDAWKDGSVSGLLA
RGNFEVGMEWSDKQATKFTITSNNGGECIAKYENIGLSVIRDSKGNLVNYTVLEDEKISF
DTVKGETYTITNIPTSKLEAPNKLKAYRIGDSIVDLEWNAVEGKDVTYNVYRQIDNGDLV
LVGKNVSTTTFEDKNSQDILGTFKYSISATIDGNETELSDFVYVKDLRNMEGYIDDSDPR
VTYVGNWGNWSEAVNHEGTVKFLDNPTGTETAELTFLGTGIEVLVVTNYDRGKYEVFIDG
VSKGEVDTYSPSTVRKKIIYSNYDLPKGRHTIKLRATATKNNSASRAKVELDAFKVIDNT
APVVTNIDVFSNSGIYTLSKANSKLQMIANVSPVDAKNKEVSWKVTDASGNTTNLASINE
KGLLTINSSNGTVKVTATSKENIEISKSIDVKISIPNSIKEVISEDAINNNGWIKNTAFT
WEGNWSTWTGEPTKHHGSTKTESSTIGANIEYTFNGTGIEVYVHKHTNFSSFDISIDGGV
ATNYSLEGNNIAQTLLASFKNLQNGSHTIKLIAKERAGKNQVNIDYFKVFATQENSPLDK
NDLQTSIENNSNKVEKDYTEETWRAFKEAYDNSINIMNNDNSTDSDIKAATKALNDTAIA
LIEKSLTTPLVIGDGKVEAIGIESSTLMLSWTLIPDSVRYNIYKVDNVTGRESLLNSTTN
LYYRVDNLLPKTEYEFKVKAVNKNNIEALFPCIKITTNDVLDKERPNDVNNLKVNKNTIS
TAIISWDKATDNIGVKGYKVYVNGVILGTTTNTNYELTELEYGKSYVVKIIAFDEAGNTS
LNSDMLEIKADESEEAPSEIDKKVLRAVVDYAEDIKENGELENVVPAVVNEFYEALENAK
TILADKNATESQVDVVTKRFINVIHMLDFKKDDKAELKKLIEVINVLDERQYTALTWEKL
QTELEKANTIIADENAIEVEKVYEDLYKAFSNLEIEVYADRSKLQQLLSELEGKDTSKYT
SGSVNKFNLNLFNVKSVLNNRDATQEQINEAYNNLIVAYLNLRLIPDKVKLEELINKARV
LDESKYTTESINRLNLQISNSKAVLSNEEVTQKEVDEAEKELELTLANLELVKGNDSGSA
IDSPVNNSNDKSDINGKNNNSNELPNTGEVSAALIGVYGTIILLVGVYIFKKK

Enzyme Prediction     

EC	3.2.1.63

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH95	39	815	3.4e-252	0.9861495844875346

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam14498	Glyco_hyd_65N_2	1.53e-66	44	317	1	233	Glycosyl hydrolase family 65, N-terminal domain. This domain represents a domain found to the N-terminus of the glycosyl hydrolase 65 family catalytic domain.
cd00063	FN3	3.47e-07	1395	1477	5	93	Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all animal proteins contain the FN3 repeat; including extracellular and intracellular proteins, membrane spanning cytokine receptors, growth hormone receptors, tyrosine phosphatase receptors, and adhesion molecules. FN3-like domains are also found in bacterial glycosyl hydrolases.
smart00060	FN3	7.07e-07	1397	1466	7	82	Fibronectin type 3 domain. One of three types of internal repeat within the plasma protein, fibronectin. The tenth fibronectin type III repeat contains a RGD cell recognition sequence in a flexible loop between 2 strands. Type III modules are present in both extracellular and intracellular proteins.
cd00063	FN3	9.87e-06	1486	1557	1	81	Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all animal proteins contain the FN3 repeat; including extracellular and intracellular proteins, membrane spanning cytokine receptors, growth hormone receptors, tyrosine phosphatase receptors, and adhesion molecules. FN3-like domains are also found in bacterial glycosyl hydrolases.
pfam17996	CE2_N	1.14e-05	1227	1292	19	81	Carbohydrate esterase 2 N-terminal. This is the N-terminal beta-sheet domain with jelly roll topology found in CE2 acetyl-esterase from the bacterium Clostridium thermocellum. This enzyme displays dual activities, it catalyses the deacetylation of plant polysaccharides and also potentiates the activity of its appended cellulase catalytic module through its noncatalytic cellulose binding function. This N-terminal jelly-roll domain appears to extend the substrate/cellulose binding cleft of the catalytic domain in C.thermocellum.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QHB24557.1	0.0	38	1561	40	1556
QEI32060.1	0.0	38	1561	40	1556
QRT30709.1	0.0	38	1561	40	1556
ASM69030.1	0.0	42	1561	41	1551
QNM13266.1	0.0	57	1783	74	1709

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2EAB_A	3.51e-219	39	851	15	895	Crystalstructure of 1,2-a-L-fucosidase from Bifidobacterium bifidum (apo form) [Bifidobacterium bifidum],2EAB_B Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum (apo form) [Bifidobacterium bifidum],2EAC_A Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complex with deoxyfuconojirimycin [Bifidobacterium bifidum],2EAC_B Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complex with deoxyfuconojirimycin [Bifidobacterium bifidum]
2EAD_A	2.62e-218	39	851	15	895	ChainA, Alpha-fucosidase [Bifidobacterium bifidum],2EAD_B Chain B, Alpha-fucosidase [Bifidobacterium bifidum]
2EAE_A	4.96e-218	39	851	14	894	ChainA, Alpha-fucosidase [Bifidobacterium bifidum]
4UFC_A	1.11e-125	37	805	17	736	Crystalstructure of the GH95 enzyme BACOVA_03438 [Bacteroides ovatus],4UFC_B Crystal structure of the GH95 enzyme BACOVA_03438 [Bacteroides ovatus]
2RDY_A	2.80e-124	42	850	3	789	ChainA, BH0842 protein [Halalkalibacterium halodurans C-125],2RDY_B Chain B, BH0842 protein [Halalkalibacterium halodurans C-125]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8L7W8	2.03e-100	39	802	49	804	Alpha-L-fucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=FUC95A PE=1 SV=1
A2R797	9.33e-87	44	792	23	758	Probable alpha-fucosidase A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=afcA PE=3 SV=1
Q5AU81	1.39e-75	64	793	46	778	Alpha-fucosidase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=afcA PE=1 SV=1
Q2USL3	7.83e-63	64	793	35	697	Probable alpha-fucosidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=afcA PE=3 SV=2
P20533	2.87e-13	1387	1569	463	643	Chitinase A1 OS=Niallia circulans OX=1397 GN=chiA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000314	0.998984	0.000186	0.000177	0.000158	0.000149

TMHMM  Annotations     

start	end
7	26
1888	1910