CAZyme Information: MGYG000004230_01188

Basic Information

• Species: Clostridium sp900759995
• Lineage: Bacteria; Firmicutes_A; Clostridia; Clostridiales; Clostridiaceae; Clostridium; Clostridium sp900759995
• CAZyme ID: MGYG000004230_01188
• CAZy Family: GH84
• CAZyme Description: Hyaluronoglucosaminidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1746	MGYG000004230_24|CGC1	197536.96	4.6502

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004230	3516057	MAG	United Kingdom	Europe

• Gene Location: Start: 1517; End: 6757 Strand: +

Full Sequence      

MIKKSKEYLALMLTSLILLSNMPGSIKVSAETKTEKIEIKQTEDSKYEIYPAPHKISYEE
GNLSFSSGVNVIFESGIDNYTKSRLENEILKVHNISYEVSKTNEIANDKTNILVGINGSG
EVVDSYSRKYAIETENLFEKNDSYFLAADKNVITILGRDTDSAFYGLTTLYHILNQKNDN
SIKNFKIEDYANVVTRGFIEGYYGNPWSVQDRIDLMTYGGYYKLNAYFYAPKDDPKHRVQ
WRSLYTEDELKWIKQLADAGNASKTRFVYGIHPFPGNDPFGINKNEAGYQKDLNDLKSKL
KQVIDQGVRQVAILADDFANPGGQLGLRLINDMTNWLKNEVKLQYPDMKTTLPYIPYDYM
GNGSSNEFNYLKQSPENVQIVMTGGRIWGEVSQSFSSTFKSNTGRAPYYWINWPCSDNSK
QHLIMGGNDTFLHPGVDPTLIEGIMLNPMQQSEANKSALFAVADYSWNIWESKEEADQNW
HDSFKYMDHNTAIETEASAALREISKHMINQNMDGRVRALQESLELAPKITAFKEKYSSG
AKVKEDALELIKEFKKLKDSAQYYKNNPGNEKTRDQIIYWLNSWEDTTTAAIEYLEAIIS
NEEGNKSDLWTHYSQAKSAFEKSKTYGFKYVDHIQYAEVGVQHIVPFIKNMEGILAPIVA
GIVDPSIIVPSVITNRIDLPTGNLNNILDNKPSTEVVYKNPSSISEGTYIGIKYNRLIKI
KDIEFLLGAIANPNDTMQEAKVQYTVDGSNWIDLEEGKLYTMPNKITIDKLNLEAMGIRI
IATKERTNTWLGIKDITVNKDNIADDSEIRPTVIRTQWPQIYQGSEANLFDGNDSTDVWY
KTHAGDITNVGDYIGVDLGKVIPVGEVRFVTGRNGSSDKWTSYKLEYSKDNINWTTFKEY
NGKASGQDIIEENLEGVEARYVRLTNKVALNKWIIFSEISVKRYGSNSSTKNVYTNTELE
LLSVLESEELTKLVPKENINLLDNEYIGIKLDRIKDLSEINLEVSNKDGLVLQTSMNEIE
WNDVDLENMKDLQEARYIRLMANKEVTFNLNKFEVKSNEVYPPSLKEAFVPIDGNVANIF
DKNFNTLARFNSFPIKDRSIVFDLGQTINIRNLTYAVLDTEIDYLRDAKFQISSDGENWT
DVITIGDGIQNDDTDTNSKPVECGYEHGDSTTIVPISHAFVRGELESDKEARYLRILFTA
DYMYRWIKISEILINDGEYIPTVNDPTYVSNPIELSGFSPQNIKDKNLTTAYKANTNNGE
IKSGSLTYRLSENTDVKKINIVQSGNEISNARVMVRTGYNQSGEGIWEELGRLDKSLTEL
VNMKYDNIFEIRIDWQGAAPTIYEIVTINNYSLPNITDLEELVLSSEIYKSDEYTVGSYT
KFTEALKYAKDILNNLENANQEEIDKAKESLVTSITDLVNIEELRSIIESANNLINSDTE
YTEVTLNVLKESVEAANVVLNNPDATKDAVSEAIKNVKEAINSLAIDEIIPVNKEQLKAT
VDIAEYITEEELSKVENIASVNKFREALEEARIILADEDANQESIDAATTNLKSAIEGLS
FKNNDKKPLTSLIEKIMNLNNENYTEKSWRILTEKLEMAKAVLIKENASEEEITKAYSEL
FRAFSELKLKPSKERLESLINKAEGYTKKYYTKNSFEVLSEKLKEAKKVLKDKNSTEADI
LTAEIELQKAIDQLVVNNSNNNNSNNNDKNNNNSGNLPLTGGTSTIVLGGIASLLLAAGV
VLRKKK

Enzyme Prediction     

EC	3.2.1.35	3.2.1.52

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH84	196	504	1.2e-98	0.9898305084745763
CBM32	824	927	4.9e-17	0.7419354838709677

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07555	NAGidase	2.57e-110	196	504	1	293	beta-N-acetylglucosaminidase. This family has previously been described as a hyaluronidase. However, more recently it has been shown that this family has beta-N-acetylglucosaminidase activity.
pfam02838	Glyco_hydro_20b	2.19e-21	46	189	1	123	Glycosyl hydrolase family 20, domain 2. This domain has a zincin-like fold.
pfam00754	F5_F8_type_C	2.45e-12	814	925	2	111	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
cd00057	FA58C	4.63e-12	826	934	25	135	Substituted updates: Jan 31, 2002
COG1196	Smc	5.30e-08	1385	1694	696	1003	Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAC99989.1	0.0	1	1496	1	1498
QAS62053.1	0.0	3	1695	3	1692
AYE33902.1	0.0	3	1695	3	1692
ATD54239.1	0.0	3	1695	3	1691
ATD58081.1	0.0	3	1695	3	1691

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6PV4_A	3.46e-257	47	664	32	653	Structureof CpGH84A [Clostridium perfringens ATCC 13124],6PV4_B Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_C Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_D Structure of CpGH84A [Clostridium perfringens ATCC 13124]
6PWI_A	2.07e-120	40	655	27	624	Structureof CpGH84D [Clostridium perfringens ATCC 13124],6PWI_B Structure of CpGH84D [Clostridium perfringens ATCC 13124]
5MI4_A	1.81e-52	109	618	63	544	BtGH84mutant with covalent modification by MA3 [Bacteroides thetaiotaomicron VPI-5482],5MI5_A BtGH84 mutant with covalent modification by MA3 in complex with PUGNAc [Bacteroides thetaiotaomicron VPI-5482],5MI6_A BtGH84 mutant with covalent modification by MA3 in complex with Thiamet G [Bacteroides thetaiotaomicron VPI-5482],5MI7_A BtGH84 mutant with covalent modification by MA4 in complex with PUGNAc [Bacteroides thetaiotaomicron VPI-5482]
2J4G_A	3.70e-52	109	618	51	532	Bacteroidesthetaiotaomicron GH84 O-GlcNAcase in complex with n-butyl- thiazoline inhibitor [Bacteroides thetaiotaomicron VPI-5482],2J4G_B Bacteroides thetaiotaomicron GH84 O-GlcNAcase in complex with n-butyl- thiazoline inhibitor [Bacteroides thetaiotaomicron VPI-5482]
2J47_A	3.75e-52	109	618	52	533	Bacteroidesthetaiotaomicron GH84 O-GlcNAcase in complex with a imidazole-pugnac hybrid inhibitor [Bacteroides thetaiotaomicron VPI-5482],2W4X_A BtGH84 in complex with STZ [Bacteroides thetaiotaomicron VPI-5482],2W66_A BtGH84 in complex with HQ602 [Bacteroides thetaiotaomicron VPI-5482],2W66_B BtGH84 in complex with HQ602 [Bacteroides thetaiotaomicron VPI-5482],2W67_A BtGH84 in complex with FMA34 [Bacteroides thetaiotaomicron VPI-5482],2W67_B BtGH84 in complex with FMA34 [Bacteroides thetaiotaomicron VPI-5482],2WCA_A BtGH84 in complex with n-butyl pugnac [Bacteroides thetaiotaomicron VPI-5482],2XJ7_A BtGH84 in complex with 6-acetamido-6-deoxy-castanospermine [Bacteroides thetaiotaomicron VPI-5482],2XJ7_B BtGH84 in complex with 6-acetamido-6-deoxy-castanospermine [Bacteroides thetaiotaomicron VPI-5482],2XM1_A BtGH84 in complex with N-acetyl gluconolactam [Bacteroides thetaiotaomicron VPI-5482],2XM1_B BtGH84 in complex with N-acetyl gluconolactam [Bacteroides thetaiotaomicron VPI-5482],2XM2_A BtGH84 in complex with LOGNAc [Bacteroides thetaiotaomicron VPI-5482],2XM2_B BtGH84 in complex with LOGNAc [Bacteroides thetaiotaomicron VPI-5482],4UR9_A Structure of ligand bound glycosylhydrolase [Bacteroides thetaiotaomicron],4UR9_B Structure of ligand bound glycosylhydrolase [Bacteroides thetaiotaomicron],5FKY_A Structure of a hydrolase bound with an inhibitor [Bacteroides thetaiotaomicron],5FKY_B Structure of a hydrolase bound with an inhibitor [Bacteroides thetaiotaomicron],5FL0_A Structure of a hydrolase with an inhibitor [Bacteroides thetaiotaomicron],5FL0_B Structure of a hydrolase with an inhibitor [Bacteroides thetaiotaomicron],5FL1_A Structure of a hydrolase with an inhibitor [Bacteroides thetaiotaomicron],5FL1_B Structure of a hydrolase with an inhibitor [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P26831	0.0	47	1556	39	1565	Hyaluronoglucosaminidase OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagH PE=1 SV=2
Q89ZI2	2.72e-51	109	618	73	554	O-GlcNAcase BT_4395 OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_4395 PE=1 SV=1
Q8XL08	7.26e-47	51	567	48	542	O-GlcNAcase NagJ OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagJ PE=1 SV=1
Q0TR53	2.92e-46	51	567	48	542	O-GlcNAcase NagJ OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=nagJ PE=1 SV=1
Q8VIJ5	4.85e-23	197	465	63	331	Protein O-GlcNAcase OS=Rattus norvegicus OX=10116 GN=Oga PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000488	0.989758	0.009055	0.000268	0.000222	0.000183

TMHMM  Annotations     

start	end
1720	1742