CAZyme Information: MGYG000004168_02351

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; CAG-74; Firm-11
• CAZyme ID: MGYG000004168_02351
• CAZy Family: GH2
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
824	MGYG000004168_19|CGC1	93701.67	5.703

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004168	4047630	MAG	United Kingdom	Europe

• Gene Location: Start: 16548; End: 19022 Strand: +

Full Sequence      

MKKISLDGLWRMRATDRDNWREAVVPGSVYADLLRNGELEDPFYRENELAAFELMRGGYE
YRRTFSLGGEALGFARALLRCEGLDTLAHVSVNGTEIGFAGNMHVAWEWDVKQALRAGEN
EISIRFDSAVEYALAEGAKRPGWCSSDAIPGYQHIRKAHCMFGWDWGPRLPDAGIWRGIS
LVLLDKARLESVLVEQEHQAERVILRVTPEIDGDTAACEWTASVTAPDGTVVTLDGPQTG
ASAMLTIHHPQLWWPSGYGDQPLYAVRVALTSGGREQDAWERRIGLRTLTVSREKDEWGE
EFCHIVNGVKIFAMGADYIPEDNILSRVTPARTRRLLEDAKLANYNCVRIWGGGYYPDDF
FFDICDELGLMVWQDFMYACAYYDLTPDFEKSIRLEAEQNVRRLRHHASLALLCGNNEME
QFQAEGIAAEHKGDFVSFGPSRPSHHADYIKMFEYILPEICGRLAPQTFYWPASPSSGGA
FDEPNAENRGDVHYWDVWHGEKPFTEYRKFYFRYVSEFGFQSFPCLKTVESFTLPEDRNI
FSRVMERHQRNKAANGKILSYLSQTYRYPGSFDNLLYCSQLLQADAIRYGVEHWRRNRGR
CMGAIVWQLNDCWPVASWSSIDYFGRWKALHYAAKRFFAPVLISAEEEGELTQNPQINEF
HPAPIRRSIRLNVSNETRACVSGVVKWALRGPDASVIRHGEQGLTVPALSAAWLDRLDFP
DASLTEHYFSFAFEVDGRAISKGTALFCAPKHFAFADPRLEIRREGGEVVISAQAFAKHV
YVESDDPDLLLEDNFFDLSAGERRIKVLRGDAHGLRVRSVFDLA

Enzyme Prediction     

No EC number prediction in MGYG000004168_02351.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	2	653	1.1e-106	0.6316489361702128

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	3.37e-104	1	804	10	780	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	1.16e-12	14	372	65	394	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam17753	Ig_mannosidase	5.11e-10	757	822	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
pfam17786	Mannosidase_ig	7.38e-10	669	752	3	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
pfam00703	Glyco_hydro_2	8.40e-08	187	287	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QTE69834.1	0.0	1	823	1	810
QTE73768.1	0.0	1	823	1	810
AHF25065.1	0.0	1	823	1	809
AUS95659.1	0.0	1	823	1	802
QIB55233.1	0.0	1	823	2	808

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VJX_A	1.02e-147	6	804	11	812	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	1.08e-147	6	804	13	814	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	1.11e-147	6	804	13	814	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	2.92e-147	6	804	11	812	Structureof the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]
6BYE_A	6.49e-146	1	802	3	826	Crystalstructure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306],6BYE_B Crystal structure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q4WAH4	6.12e-121	1	810	4	830	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
Q0CCA0	1.60e-120	23	818	30	839	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2
B0YBU9	2.35e-120	1	810	4	830	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1
A1D911	2.48e-119	1	810	4	830	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
Q5B7W2	3.30e-119	18	810	24	828	Beta-mannosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndB PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000055	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004168_02351.