CAZyme Information: MGYG000004159_01820

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; UMGS1623
• CAZyme ID: MGYG000004159_01820
• CAZy Family: GH25
• CAZyme Description: Lysozyme M1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
249	MGYG000004159_26|CGC1	28190.9	5.0607

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004159	2766114	MAG	United Kingdom	Europe

• Gene Location: Start: 13143; End: 13892 Strand: -

Full Sequence      

MENKKRVRIGLLIGGGVLFLGLAVLAGLFYNGILRFHYPSRERYPIRGVDVSAYQGEIDW
SVLATQDIRFAFIKATEGSGFTDRMFAANWEGAKRAGLRVGAYHFFSFDSGGDTQADRFI
AEVEKTEGMLPPVVDVEFYGDKEARPPAKEDVQRELGVLLARLEEAYGAKPILYATGKSY
ERYLKDAYPENPIWIRNIVSEPRLPDGRDWTFWQYTDRARLPGYQGEERFIDLNVFAGSE
EAFLSFGLS

Enzyme Prediction     

No EC number prediction in MGYG000004159_01820.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH25	49	223	3.1e-50	0.9887005649717514

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06413	GH25_muramidase_1	2.78e-104	44	238	1	191	Uncharacterized bacterial muramidase containing a glycosyl hydrolase family 25 (GH25) catalytic domain. Endo-N-acetylmuramidases are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues.
cd00599	GH25_muramidase	1.02e-56	47	236	1	186	Endo-N-acetylmuramidases (muramidases) are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. This family of muramidases contains a glycosyl hydrolase family 25 (GH25) catalytic domain and is found in bacteria, fungi, slime molds, round worms, protozoans and bacteriophages. The bacteriophage members are referred to as endolysins which are involved in lysing the host cell at the end of the replication cycle to allow release of mature phage particles. Endolysins are typically modular enzymes consisting of a catalytically active domain that hydrolyzes the peptidoglycan cell wall and a cell wall-binding domain that anchors the protein to the cell wall. Endolysins generally have narrow substrate specificities with either intra-species or intra-genus bacteriolytic activity.
cd06524	GH25_YegX-like	1.73e-51	47	238	1	194	YegX is an uncharacterized bacterial protein with a glycosyl hydrolase family 25 (GH25) catalytic domain that is similar in sequence to the CH-type (Chalaropsis-type) lysozymes of the GH25 family of endolysins.
COG3757	Acm	2.92e-51	44	241	61	258	Lyzozyme M1 (1,4-beta-N-acetylmuramidase), GH25 family [Cell wall/membrane/envelope biogenesis].
pfam01183	Glyco_hydro_25	1.92e-43	49	223	1	179	Glycosyl hydrolases family 25.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCI60136.1	4.03e-91	4	249	2	247
QQP77892.1	6.85e-91	4	248	2	243
AIM12703.1	8.06e-91	22	248	1	227
AFH84717.1	8.06e-91	22	248	1	227
AHK39491.1	8.06e-91	22	248	1	227

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2WAG_A	1.18e-89	42	248	12	218	TheStructure of a family 25 Glycosyl hydrolase from Bacillus anthracis. [Bacillus anthracis str. Ames]
1JFX_A	1.86e-23	46	245	5	211	Crystalstructure of the bacterial lysozyme from Streptomyces coelicolor at 1.65 A resolution [Streptomyces coelicolor]
4KRU_A	4.88e-16	46	244	20	213	X-raystructure of catalytic domain of endolysin from clostridium perfringens phage phiSM101 [Clostridium phage phiSM101]
4KRT_A	1.88e-15	46	244	20	213	X-raystructure of endolysin from clostridium perfringens phage phiSM101 [Clostridium phage phiSM101],4KRT_B X-ray structure of endolysin from clostridium perfringens phage phiSM101 [Clostridium phage phiSM101]
6ZMV_A	1.09e-12	46	223	2	188	ChainA, muramidase [Trichobolus zukalii],6ZMV_B Chain B, muramidase [Trichobolus zukalii]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8X7H0	3.97e-36	37	246	60	264	Uncharacterized protein YegX OS=Escherichia coli O157:H7 OX=83334 GN=yegX PE=3 SV=2
P76421	3.07e-35	30	246	51	264	Uncharacterized protein YegX OS=Escherichia coli (strain K12) OX=83333 GN=yegX PE=3 SV=2
Q8FFY2	8.51e-35	30	246	51	264	Uncharacterized protein YegX OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) OX=199310 GN=yegX PE=3 SV=2
P25310	4.38e-22	46	245	82	288	Lysozyme M1 OS=Streptomyces globisporus OX=1908 GN=acm PE=1 SV=1
P26836	2.75e-15	40	236	3	196	Probable autolytic lysozyme OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=lyc PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999458	0.000384	0.000014	0.000004	0.000002	0.000186

TMHMM  Annotations     

start	end
7	29