CAZyme Information: MGYG000004098_00563

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; CAG-272; UMGS902
• CAZyme ID: MGYG000004098_00563
• CAZy Family: GH43
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1251	MGYG000004098_33|CGC1	132601.1	6.5409

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004098	2178034	MAG	United Kingdom	Europe

• Gene Location: Start: 5150; End: 8905 Strand: +

Full Sequence      

MRNKIILCSNKTFALCILVALCTLLTCFTLRIDLSAADTSETIVYVHSSTGNDTAAGTSP
SAPLKTFTRAVNVLKNTGGRIVIMNAYSFPSTVTEPEHTAPITISTSDGTTNYGQSGAKL
VFGSALRYILKGNTTFEQIQIEYKKTLNFVANYNHITFGTGVITSNTDTGNGVYVVGGYQ
SPSNGVDVTKNSYITIKSGTFYAVIGGTRQLATGATNMTFTGTHNINISGGAISKVYGAS
VEKQYSKNAIITVSGGTIYQLFVGGDVSRRLNGDATVRLTGGTIHELSVNNVIGKANVYF
SGANIRAVSVSYYNDDIKQLAQAANKPKTLFYTTFYDPSAIELFGEDFDAVENLIKIFAK
ENATGSGASLSSPTSFAAAFEKASQAGGSIVVIGTITINNFTEPAHSEEIFIQGDTGAAL
SINGTYTLGGTTTIENLKLTVNNSTFQTAGNHLTVRNLSSMSGKANVTGSATLYAGNYGT
INGGNTKAKIILDGAQADVLIGGTKSAEIEIHSGTVSTLKTSESAIENFTLKMAGGGVQR
LELKNVTNSLRLELHGGSVGEYVVSGSNINGTAVLNRSFYNPSDLGAAASIFTIYDESAV
YLKNGGTGNGSTATSPLGSLSAAYTALPNGGVIVICGEYTLNQAFINVANADKITITSMY
DGVDYAKTNDAKLVLQANFYCGGETEFHDITMVCAKNYGSFFGCGHKFTLGDNIISEKAA
DINTYMSIMGGSTSDINPSATNLTINSGKWQRVRGGNSTGSTKLLAVNMTINGGEFFERV
TLGSGTAHNGTINATIDGGTFYAGIFAFTPESTQLSLSSAVTLTLNGGTFYGTLAASSTK
LGTYTGSYTVYINGGNYAHITDLSGTASLSGSMNSTLHVAENINLEAQETGTMSFTNPIR
SNGADPWLFFYEGNYYYIATGSTRLALYKAANIGDLKYAAQKIIYEPADGQMWSKNLWSP
EIHYFSSEDFGEEYAGWYCFIACDDGENVNHRMYVIKCLTDDLQGPWGHPVTGEINVPIK
VTSPSNPTLTNSTWAAGQSIIRINNQLYTLWVGEVGRGTSNFYQTINIARLSNPWTITGT
HSVICTPEYSWEMGGAAYNATTGASYPKVVEGGTAVYGSGGSIYIVYSGSGYWTTEYKLG
QLKYLGGDPMSKSSWQKLSTPIFAKSESINGCGHASYVTDTSSNTWICYHAYIGTDTSSG
RSAFVEPYTATTSGVTIGNGTKKPASIHTVYTVNLNPRPLTNKISGFGSII

Enzyme Prediction     

No EC number prediction in MGYG000004098_00563.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH43	895	1214	2.3e-72	0.9897959183673469

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd08980	GH43_LbAraf43-like	7.68e-74	904	1192	1	276	Glycosyl hydrolase family 43 proteins such as Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans GbtXyl43B. This glycosyl hydrolase family 43 (GH43) subgroup includes enzymes with beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55) and possibly bifunctional xylosidase/arabinofuranosidase activities. In addition to Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans IT-08 beta-xylosidase / exo-xylanase (GbtXyl43B). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) familiesGH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
cd18820	GH43_LbAraf43-like	7.07e-72	904	1191	1	257	Glycosyl hydrolase family 43 proteins similar to Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans GbtXyl43B. This uncharacterized glycosyl hydrolase family 43 (GH43) subgroup belongs to a subgroup which includes enzymes with beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55) and possibly bifunctional xylosidase/arabinofuranosidase activities, similar to Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans IT-08 beta-xylosidase / exo-xylanase (GbtXyl43B). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
cd18817	GH43f_LbAraf43-like	2.31e-37	904	1191	1	261	Glycosyl hydrolase family 43 such as Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43. This glycosyl hydrolase family 43 (GH43) subgroup includes characterized enzymes with alpha-L-arabinofuranosidase (EC 3.2.1.55) activity. It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. Characterized enzymes belonging to this subgroup include Lactobacillus brevis (LbAraf43) and Weissella sp (WAraf43) which show activity with similar catalytic efficiency on 1,5-alpha-L-arabinooligosaccharides with a degree of polymerization (DP) of 2-3; size is limited by an extended loop at the entrance to the active site. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
COG3940	COG3940	2.47e-32	897	1191	7	277	Beta-xylosidase, GH43 family [Carbohydrate transport and metabolism].
cd18818	GH43_GbtXyl43B-like	1.14e-31	904	1191	1	272	Glycosyl hydrolase family 43 such as Geobacillus thermoleovorans IT-08 beta-xylosidase/exo-xylanase (GbtXyl43B). This glycosyl hydrolase family 43 (GH43) subgroup includes the characterized enzymes Geobacillus thermoleovorans IT-08 beta-xylosidase (EC 3.2.1.37) / exo-xylanase (GbtXyl43B), and Paenibacillus sp. strain E18 alpha-L-arabinofuranosidase (EC 3.2.1.55) Abf43B. It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QOR70069.1	1.97e-181	37	1247	29	1275
QOR70064.1	2.01e-180	41	1247	33	1273
QMU96449.1	5.37e-173	42	1249	35	1277
SDS43825.1	8.77e-170	35	1247	26	1273
ACQ82437.1	3.54e-166	36	1247	27	1273

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3AKF_A	1.54e-21	885	1190	1	278	Crystalstructure of exo-1,5-alpha-L-arabinofuranosidase [Streptomyces avermitilis MA-4680 = NBRC 14893],3AKG_A Crystal structure of exo-1,5-alpha-L-arabinofuranosidase complexed with alpha-1,5-L-arabinofuranobiose [Streptomyces avermitilis MA-4680 = NBRC 14893],3AKH_A Crystal structure of exo-1,5-alpha-L-arabinofuranosidase complexed with alpha-1,5-L-arabinofuranotriose [Streptomyces avermitilis MA-4680 = NBRC 14893],3AKI_A Crystal structure of exo-1,5-alpha-L-arabinofuranosidase complexed with alpha-L-arabinofuranosyl azido [Streptomyces avermitilis MA-4680 = NBRC 14893]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q82P90	1.26e-20	887	1190	29	304	Extracellular exo-alpha-(1->5)-L-arabinofuranosidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=Araf43A PE=1 SV=1
G4MMH2	1.56e-11	893	1191	39	311	Alpha-L-arabinofuranosidase B OS=Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) OX=242507 GN=abfB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.190319	0.361793	0.446320	0.000424	0.000358	0.000789

TMHMM  Annotations     

start	end
13	32