CAZyme Information: MGYG000004085_00889

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Monoglobales_A; UMGS1253; UMGS1253
• CAZyme ID: MGYG000004085_00889
• CAZy Family: PL35
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1406	MGYG000004085_3|CGC3	155929.24	4.2637

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004085	2144710	MAG	United Kingdom	Europe

• Gene Location: Start: 91396; End: 95616 Strand: +

Full Sequence      

MAKKIVSLVLAMTMVLSLFSTLSFAADDETFTIVDMNKKSVITGAGAKASSKETKDAKYS
AQWDMDKLNDLIFKDIEHDMSQYSKISFNIKLDSTGPATISFALHSENNGTDGIDYYSKE
LTLTPGDWATVELNYSDLMKNREPKGYNDIDNITLHGIGWNNKHEAGAVAYVDSIVLSGA
PTDITGSGNTGTDKPNSNGGKTFTEDDTEVGEKIFYNGKASGMDGLGSNEKTNKIEGGKD
DDGANYIYFETLDTNSDYHLDKTISGATRFMVIQMKLAYEKNCVSGNIQYKDSSSTTTEL
MKINNGVLTTGNKEIAKLNSKGEYTDIAFAIDWVDNSADVYVDGELVEEGLIFASPDRDA
ISSLRIYVGSGNAVGSNLLVKDYLVYEGTEPREISKDDLGPRVSVVAQDNSAAIKTLGDN
VALALGGNGIYYNREKHDIDAPAFETGDRTLVPVRAISEAFGLQVDWDEATGTVTIDGKS
KIVIGSTEMILPDGSTYTLDVPAETYNDRTFLPLRALCEQILGKVVTWDDHGLIVIGDAE
FTASENALVEAFNYLLYDRPMADELVQIFNEQNQNQHPRVMMNKEIYDRVVYNYANDELV
KEWGDAIIRQADNYLNAAMPTYSIPDGYRLLATSRDVYGRSKVLSMAYILTKDKKYSDNL
YQVFTAAGNFPDWNQQHFLDIGEMTCAFAIGYDWLYDVWTDEQRAFLEDKIYNYGLVVAD
KAYYSQLGGDGWWTPGNTTNWNVVCNGGILMGATAIFDKYPEFCANLIQLEVRDVEAMMN
SFYPAGAWFEGIGYWSYTLDYTVNMFSTLQACFGTDFNLTKAPGFDRTVYFSMAGDGPVS
INNYHDAGEGHQNSSTYFWLSNQFDIPGVTNVRLFNMEEFGWSPSPFDLLWYNTDIKGTD
FELEKDTYLPEVEFVAMRNSWVDNEGAWLSFHAGQANVNHSHLDTGSFVVDLLGERWAVD
LGGDDYNMDGYFGGNKHKYYRLRPEGHNLYVINPSEYEGQDVNSFCKVENGVVSKPRGAY
AIADLTPAYARDASSARRGYMLADDRRSALIRDEITFKSGENDFYWFSHISSGTEAEIVD
NNTVILTKNGKKVKVLIDTNLPDYELSIMDAVPLPSSPVMPAQNKNEGIHKIAIHSPKAS
GSVYVQVKFIPVDDVNADKPLENVALDDWTIPDGDFVPAPIAPTLTDLAKNGETLTAFEP
KQTGYTVSVPYGTTEVPVITAAAEADCDVVIEQSQSFDTPTIVYVSRKDEPDVVRKYTIG
YTVLPKLEDVNGWKRLQVSGHSASVEPEAEHPATQVSNNDTSAESRWAGEGTQWVQIDLG
SVQSVSAIGLSWWKGNERAYSFDIEVSTDGVNFEPVLTEKSSDGKTEGYEIFELDKAYEV
RYIRYVGYGNSANNWNSVTEFAALTK

Enzyme Prediction     

No EC number prediction in MGYG000004085_00889.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL35	925	1098	1.9e-42	0.9664804469273743
CBM32	1288	1402	4.4e-20	0.8790322580645161

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07833	Cu_amine_oxidN1	6.75e-24	447	536	2	92	Copper amine oxidase N-terminal domain. Copper amine oxidases catalyze the oxidative deamination of primary amines to the corresponding aldehydes, while reducing molecular oxygen to hydrogen peroxide. These enzymes are dimers of identical subunits, each comprising four domains. The N-terminal domain, which is absent in some amine oxidases, consists of a five-stranded antiparallel beta sheet twisted around an alpha helix. The D1 domains from the two subunits comprise the 'stalk' of the mushroom-shaped dimer, and interact with each other but do not pack tightly against each other.
pfam07833	Cu_amine_oxidN1	7.52e-15	399	476	16	93	Copper amine oxidase N-terminal domain. Copper amine oxidases catalyze the oxidative deamination of primary amines to the corresponding aldehydes, while reducing molecular oxygen to hydrogen peroxide. These enzymes are dimers of identical subunits, each comprising four domains. The N-terminal domain, which is absent in some amine oxidases, consists of a five-stranded antiparallel beta sheet twisted around an alpha helix. The D1 domains from the two subunits comprise the 'stalk' of the mushroom-shaped dimer, and interact with each other but do not pack tightly against each other.
pfam00754	F5_F8_type_C	4.46e-14	1282	1401	3	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam07940	Hepar_II_III	0.002	940	1090	39	185	Heparinase II/III-like protein. This family features sequences that are similar to a region of the Flavobacterium heparinum proteins heparinase II and heparinase III. The former is known to degrade heparin and heparin sulphate, whereas the latter predominantly degrades heparin sulphate. Both are secreted into the periplasmic space upon induction with heparin.
cd00057	FA58C	0.003	1313	1384	46	125	Substituted updates: Jan 31, 2002

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUH30172.1	9.30e-148	575	1399	71	888
AZS17848.1	4.39e-145	289	1400	133	1273
ALS27107.1	3.75e-126	556	1402	677	1509
SDS98549.1	1.08e-120	577	1400	52	854
QEH43197.1	1.49e-116	574	1149	30	600

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5ZU6_A	4.07e-23	1300	1404	52	157	ACBM32 derived from alginate lyase B (AlyB-OU02) [Vibrio]
5ZU5_A	1.07e-20	1300	1404	52	157	Crystalstructure of a full length alginate lyase with CBM domain [Vibrio splendidus]
7D29_A	1.63e-14	1300	1402	29	130	CBM32of AlyQ [Persicobacter sp. CCB-QB2],7D2A_A CBM32 of AlyQ in complex with 4,5-unsaturated mannuronic acid [Persicobacter sp. CCB-QB2]
5XNR_A	7.11e-13	1300	1402	29	130	TruncatedAlyQ with CBM32 and alginate lyase domains [Persicobacter sp. CCB-QB2]
2JD9_A	3.93e-10	1300	1402	31	132	Structureof a pectin binding carbohydrate binding module determined in an orthorhombic crystal form. [Yersinia enterocolitica],2JDA_A Structure of a pectin binding carbohydrate binding module determined in an monoclinic crystal form. [Yersinia enterocolitica],2JDA_B Structure of a pectin binding carbohydrate binding module determined in an monoclinic crystal form. [Yersinia enterocolitica]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000245	0.999125	0.000162	0.000168	0.000155	0.000144

TMHMM  Annotations     

start	end
5	27