CAZyme Information: MGYG000004055_00239

Basic Information

• Species: Eubacterium_G sp900548465
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Eubacterium_G; Eubacterium_G sp900548465
• CAZyme ID: MGYG000004055_00239
• CAZy Family: GH16
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1205	MGYG000004055_1|CGC1	129416	9.3778

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004055	2465387	MAG	United Kingdom	Europe

• Gene Location: Start: 265595; End: 269212 Strand: +

Full Sequence      

MKKRILSVVLTVAMVLASLVYQPAIKEVKAAAWNLVWSDEFDGNSLDTSVWTRETGGSDG
GGWGNNELEYYTDRTDNSYVSDGTLKIVARRENYGGCRFTSARLKSAGKKSFKYGRMEAR
IKVNGGNQDGVWPAFWMMGDDGTPWPWCGELDIMEHANSRNYVEGTIHWNAGGSSYGTAY
NHIFWGSYSVPAYYYYSDNTNNGITGWHTYGVVWTDSTIQWYVDNHVYLTAHLTPDNAYA
FQKNHYFLLNLALGSNSTGYTGNVAPNDSFRSATMEVDYVRVFSGSGDLNDTNIGGTVTK
PTTPSDGYTALANNTVTTIGDCSLYAGNWDNGAISYKGSKLSDLNVRLDSTSGNPGTWGT
QLKYPMNLIQGKKYNVTLKFDSTKSGTMYWKNEGSSDTVDLGTAQYAVNAGTNTKTIQIT
ADSKNTAVMEMSGMPAGTVLNNISISAIQEADETTAKPAYQPQPGNFDAFNTIEAENYTS
NNGGVIDENSSAGNGHNIGGILDKTSLTYGTVNFTEPASSINFQYSCPQDCGGTADVYCD
GTKVGTVTLNSNASSWNEYGNVTAKLDSVITTGVHSIKLLFNTKDGSAYACNLDYFSFNK
ASEDQFVPSGYTTATVNQWMTSGKWGCYFGNWNGTASGSYKWISDADYNIYVDQANKGTE
WLVQGSYTGNVTSGHTYKVSVDVTSTKDCSVGMKEGISNDKETQVYTDLTANSTKTLTGT
YKVTNNQIKVMFELGKSVESGTNINFKKIKIEDITSGITNPQTTVAPTTKEAPTASPTTV
APTVVGEDDSIPAPVGLTYAGNETLPYYFAWQSPADDIESYNVYVDGKFVATSNVAAINL
TSEAFSSGNGDYIVSVRSVRNGKESKATSITYTFAGGSGEKPTTVVPTTVAPTTKAVPTT
VAPTTKTPTTVAPTTKAVPTTVAPTTKAVPTTVAPTTKAVPTTVAPTTKAVPTTVAPTTK
AVPTTVAPTTKAVPTTVAPTTKEMPTEFATTTEASVDKGILNTDENGINEVNNKISKLNA
DKGDMAGSSFAKVMLKAKSGKKKVSLSWKKVTGATEYIIYGSACGKNNKVVKIKTVKGKK
VSIKKLKKGKFYKYMIVAVNGNKVVAVSKVCHIATKGGKYGNVNKITLNKKKITLTKGKK
FKIKAKAKSDKKVRNHIAKFRYESTNTNVAEVSSKGVVKAIGKGKTVIYVFGQNGISQKI
KVAVK

Enzyme Prediction     

No EC number prediction in MGYG000004055_00239.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	37	283	1e-71	0.9956521739130435
CBM6	472	600	2.4e-21	0.8840579710144928

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd08023	GH16_laminarinase_like	2.37e-78	37	283	1	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
cd02182	GH16_Strep_laminarinase_like	3.25e-46	33	284	2	259	Streptomyces laminarinase-like, member of glycosyl hydrolase family 16. Proteins similar to Streptomyces sioyaensis beta-1,3-glucanase (laminarinase) present in Actinomycetales as well as Peziomycotina. Laminarinases belong to glycosyl hydrolase family 16 and hydrolyze the glycosidic bond of the 1,3-beta-linked glucan, a major component of fungal and plant cell walls and the structural and storage polysaccharides (laminarin) of marine macro-algae. Members of the GH16 family have a conserved jelly roll fold with an active site channel.
cd00413	Glyco_hydrolase_16	2.04e-34	39	283	1	210	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
cd08024	GH16_CCF	2.15e-33	35	282	1	328	Coelomic cytolytic factor, member of glycosyl hydrolase family 16. Subgroup of glucanases of unknown function that are related to beta-GRP (beta-1,3-glucan recognition protein), but contain active site residues. Beta-GRPs are one group of pattern recognition receptors (PRRs), also referred to as biosensor proteins, that complexes with pathogen-associated beta-1,3-glucans and then transduces signals necessary for activation of an appropriate innate immune response. Beta-GRPs are present in insects and lack all catalytic residues. This subgroup contains related proteins that still contain the active site and are widely distributed in eukaryotes. Their structures adopt a jelly roll fold with a deep active site channel harboring the catalytic residues, like those of other glycosyl hydrolase family 16 members.
COG2273	BglS	3.30e-27	29	282	37	262	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CUU48455.1	3.44e-61	10	283	7	263
QAA30348.1	4.27e-61	3	283	5	262
AQS05577.1	7.34e-61	10	387	7	355
AVK46915.1	7.34e-61	10	387	7	355
AJG99221.1	9.99e-61	10	387	7	355

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6JH5_A	6.67e-50	33	283	4	231	Structureof Marine bacterial laminarinase [Aquimarina]
6JHJ_A	4.25e-49	33	283	4	231	Structureof Marine bacterial laminarinase mutant-E135A [Aquimarina],6JIA_A Marine bacterial laminarinase mutant E135A complex with laminaritetraose [Aquimarina sp.],6M6P_A Structure of Marine bacterial laminarinase mutant E135A in complex with 1,3-beta-cellotriosyl-glucose [Aquimarina sp.]
4CRQ_A	2.10e-44	33	283	3	230	Crystalstructure of the catalytic domain of the modular laminarinase ZgLamC mutant E142S [Zobellia galactanivorans],4CRQ_B Crystal structure of the catalytic domain of the modular laminarinase ZgLamC mutant E142S [Zobellia galactanivorans],4CTE_A Crystal structure of the catalytic domain of the modular laminarinase ZgLamC mutant E142S in complex with a thio-oligosaccharide [Zobellia galactanivorans],4CTE_B Crystal structure of the catalytic domain of the modular laminarinase ZgLamC mutant E142S in complex with a thio-oligosaccharide [Zobellia galactanivorans]
3ATG_A	2.65e-39	31	295	1	251	endo-1,3-beta-glucanasefrom Cellulosimicrobium cellulans [Cellulosimicrobium cellulans]
3ILN_A	3.14e-39	33	283	5	247	ChainA, Laminarinase [Rhodothermus marinus],3ILN_B Chain B, Laminarinase [Rhodothermus marinus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P45798	1.10e-37	29	283	36	282	Beta-glucanase OS=Rhodothermus marinus OX=29549 GN=bglA PE=1 SV=1
Q27082	6.81e-37	33	296	25	268	Clotting factor G alpha subunit OS=Tachypleus tridentatus OX=6853 PE=1 SV=1
P23903	7.18e-35	31	283	421	679	Glucan endo-1,3-beta-glucosidase A1 OS=Niallia circulans OX=1397 GN=glcA PE=1 SV=1
C1IE32	6.81e-28	32	281	19	265	Glucan endo-1,3-beta-glucosidase OS=Cryptopygus antarcticus OX=187623 PE=1 SV=1
Q9ZG90	5.40e-17	33	283	56	287	Keratan-sulfate endo-1,4-beta-galactosidase OS=Sphingobacterium multivorum OX=28454 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000323	0.998895	0.000190	0.000211	0.000179	0.000161

TMHMM  Annotations     

start	end
5	25