CAZyme Information: MGYG000004022_00004

Basic Information

• Species: HGM13222 sp900757485
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; HGM13222; HGM13222 sp900757485
• CAZyme ID: MGYG000004022_00004
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
605	MGYG000004022_1|CGC1	69494.2	5.43

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004022	2646660	MAG	United Kingdom	Europe

• Gene Location: Start: 5023; End: 6840 Strand: +

Full Sequence      

MAVYHFTKAVISKGFWAEKQKLIRETTIWNVYKRFSETGRFEAFKLNWKEGMPNKPHIFW
DSDVAKWLESVAYLTEIRREPKLEAIVDDIVDDIERGRMKDGYFNSCYQTQMPEKRFLFR
PNHELYCAGHLIEAAIAYDKATGKSKFLNLMKDYASLIKKVFMEEKSAGFTSPGHEEIEL
ALIKLYDYTKESQWLDLAKFFIDERGVHEKEEQGDSNIQDHMPVRSQETAEGHAVRACYL
YSAMADIALRTNDSELKAACDRIFDNIVNRKMYITGAIGSSHRGEVFEDDYRLPNDTAYA
ETCAAIALAFFAHRMTCFDADSVYADTIERVIYNGFLSGISLDGKSFFYENAQEIDLENR
KIVRAAIHDNHDMHFPITQRVEVFECSCCPPNVTRFISSIGDFIYHCSEDTLFINQYMDS
ETSAFGAKIIQKTDFPYDGRISVSVDNNIKLALRIPQWCKVWSVLKNGVPEKCTPVKGYA
YIDAEAGDEIILDMKLEVRFIKANHLVRADRGMTAVTYGPFVMCLEAVDNNHGTLFDVKL
TDESIRVFIDDKLHLPCVEISAVHEIVRGLYSDEVDIVPFTAKFIPYFAFANRGEADMRI
WLPKK

Enzyme Prediction     

No EC number prediction in MGYG000004022_00004.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	11	524	2.5e-171	0.9942748091603053

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	7.50e-153	8	605	15	588	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	2.99e-136	13	524	6	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QHQ63226.1	1.15e-186	6	601	11	621
AUS96918.1	3.62e-186	6	601	14	624
AZN43118.1	5.38e-156	11	605	20	657
ADH61394.1	1.63e-147	11	605	19	648
QDP39470.1	5.40e-147	8	605	10	642

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	5.94e-146	58	605	73	648	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	3.27e-139	58	605	73	648	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	1.35e-113	58	601	72	655	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	1.81e-113	58	601	72	655	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]
7EXU_A	5.04e-113	58	601	72	655	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	7.37e-113	58	601	72	655	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000056	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004022_00004.