CAZyme Information: MGYG000003967_01603

Basic Information

• Species: UMGS1338 sp900550805
• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; QALW01; UMGS1338; UMGS1338 sp900550805
• CAZyme ID: MGYG000003967_01603
• CAZy Family: GH28
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1262	MGYG000003967_22|CGC1	138907.67	4.933

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003967	2064493	MAG	United Kingdom	Europe

• Gene Location: Start: 30152; End: 33940 Strand: +

Full Sequence      

MKRTVAFWTAVLMLLLLMPLGVSAEPFGDWNTSGWKAAGDGVLASTEGKDLSVLYSKGAA
GGNCLEFDVYIKKVTGDNDANVGAAYKSGDIQYFFEYNTKSNLARIRRLGNGIDEQAGDG
KSVELKQNAWHHFMIVFDVGCVELYINGSLITAGRYTADDKMTGGTCYIQSYYTDIELKN
VAFSKVKITPSDNKQENAMEYDFEFTKKSSVEGFKAENGRVSYSGGALMYTLNGQGYLES
PIIRADKGTAYSAEMTVKNTIFMRLCNKTGADKFRVYFTTAQDGTYNEEKSRVFDVPADG
KWHSCFFNFSGIAGAKSYLRGFRIEPIGAAGGSIAIKSVTFEREKEFYDYAGEITYCTAD
KNNVVVQGRAAEKFEGKEVFLYELDTVNYTQSFAGLEPVASAKVSGGRFTITVPLKGEKV
TRLSSLFLAQVDGVKISDRFMVENWRDFTENPYAFTLPDRTVNVTDKEFGAQGDAYTDDT
KAIQKAIDAVSAAGGGTVIVPGDDSFYGRRYIATNIKLKDNVELRIEKGAVLWQSPRVKD
YAYEPAFGHDVSIPGVNWTLAASCHNYPLLQGDNVKNVRITGGGTVRSVDTGGENSDSVN
SNTIWIGCENRLHLVPIGFWKCENVEISDITLARTNNYHINMRTSRNVYIGNVEMKEVTC
ASGDGISATVGTKNLMIDRCVLYTNDDAVTICSTYNDPRGLAWWHANPDGDNCVENIVVA
HSNLHGGHGITFIPWGTDAPDLSKQEIRNIEVYDSVLGGGMMSVGSWPDNPYYGKQPYDN
SEKDDFSPVKDVYMHDNYYKGLCDLEALKATNFIADCGLHSAENFLNGDFERKNGKEGWV
SGLTNWSVSGDPEVGVTEKDGGHAGYIASEGQLFQGLYMRPGKHVFTMEATLESGAGFVF
VRSSEGEMFRQELKAGEKTEISYEFSNITGFEGRLGVEMTQKGRIVIDNANVKTEKPEYP
KYFTEDFQTRTEPTFDYDSWKLATEGDNTYLTLPTGEVGIHSLMMNGNYRDFDLKYAIRA
GKVAYGSQGNIAVSVCRRNSGTQYYVEYDMVTSLFSVRRFTGNVEKKTARKSLKLSTGEW
HTFGLRNKEGKLEFYLDGQLIMEATDSKPLSAAALGIFSYNTAIDIDCITLSEAGTQDMS
STINVNAKKWKLEFDACGGEPTPDDQYLEAGQKPMEVADPVKEGYVFAGWAYNGQKVVLS
EFTMPEGDAVLTAQWSTAQSADNSGTPWAVIGAVGGAVVLACVIVALTAGKRRKKPDDKP
QA

Enzyme Prediction     

No EC number prediction in MGYG000003967_01603.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH28	513	759	9.6e-21	0.5815384615384616

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG5434	Pgu1	4.47e-21	456	729	77	344	Polygalacturonase [Carbohydrate transport and metabolism].
PLN02218	PLN02218	5.85e-07	461	691	67	269	polygalacturonase ADPG
pfam12708	Pectate_lyase_3	2.55e-06	462	524	2	58	Pectate lyase superfamily protein. This family of proteins possesses a beta helical structure like Pectate lyase. This family is most closely related to glycosyl hydrolase family 28.
pfam00295	Glyco_hydro_28	1.52e-05	566	731	42	185	Glycosyl hydrolases family 28. Glycosyl hydrolase family 28 includes polygalacturonase EC:3.2.1.15 as well as rhamnogalacturonase A(RGase A), EC:3.2.1.-. These enzymes are important in cell wall metabolism.
pfam09479	Flg_new	2.53e-05	1154	1214	5	65	Listeria-Bacteroides repeat domain (List_Bact_rpt). This model describes a conserved core region of about 43 residues, which occurs in at least two families of tandem repeats. These include 78-residue repeats which occur from 2 to 15 times in some proteins of Bacteroides forsythus ATCC 43037, and 70-residue repeats found in families of internalins of Listeria species. Single copies are found in proteins of Fibrobacter succinogenes, Geobacter sulfurreducens, and a few other bacteria.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGA23464.1	6.89e-189	205	943	71	787
QCD41820.1	3.57e-110	251	884	248	873
ADB48561.1	2.37e-46	462	897	28	442
ADB48560.1	6.04e-40	462	894	28	449
QGQ94767.1	1.60e-38	462	887	37	454

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5OLP_A	9.13e-15	454	754	37	324	Galacturonidase[Bacteroides thetaiotaomicron VPI-5482],5OLP_B Galacturonidase [Bacteroides thetaiotaomicron VPI-5482]
4MXN_A	6.21e-11	469	669	27	210	Crystalstructure of a putative glycosyl hydrolase (PARMER_00599) from Parabacteroides merdae ATCC 43184 at 1.95 A resolution [Parabacteroides merdae ATCC 43184],4MXN_B Crystal structure of a putative glycosyl hydrolase (PARMER_00599) from Parabacteroides merdae ATCC 43184 at 1.95 A resolution [Parabacteroides merdae ATCC 43184],4MXN_C Crystal structure of a putative glycosyl hydrolase (PARMER_00599) from Parabacteroides merdae ATCC 43184 at 1.95 A resolution [Parabacteroides merdae ATCC 43184],4MXN_D Crystal structure of a putative glycosyl hydrolase (PARMER_00599) from Parabacteroides merdae ATCC 43184 at 1.95 A resolution [Parabacteroides merdae ATCC 43184]
3JUR_A	2.92e-10	457	727	23	298	Thecrystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_B The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_C The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_D The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P35336	1.09e-07	460	754	88	363	Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000212	0.999142	0.000158	0.000169	0.000149	0.000142

TMHMM  Annotations     

start	end
5	24
1228	1250