CAZyme Information: MGYG000003924_00718

Basic Information

• Species: Mailhella sp900555975
• Lineage: Bacteria; Desulfobacterota; Desulfovibrionia; Desulfovibrionales; Desulfovibrionaceae; Mailhella; Mailhella sp900555975
• CAZyme ID: MGYG000003924_00718
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 1A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
868		95696.45	6.8935

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003924	2228813	MAG	China	Asia

• Gene Location: Start: 3548; End: 6154 Strand: +

Full Sequence      

MSNNDRYNDHGPDWLDLGTPDAGRKKRLFRRERDADEDDILTLDDPVSTPNRPKKPKKRG
LFRKFLYFLFSLMLIGVLRAAGGGYMLYQWVADDLPSFSKIADYRPPLVTTVLARDGSVI
GQLYRERRFLVTLDQLPKFVPLSFLAVEDDQFYNHPGVDIKAIIRAAIANFQHGSTRQGG
STITQQVVKRLMLTPEKSYERKLKEAILAFRLEKQLSKDDILTLYINQIFLGNNAYGVEA
AARVYFAKNARDLTIAEAALIAGLGQSPSAYNPYRNPEAAETRQRHVLRRMRDLGWINDA
EYDEAVNQKLEYKAMQSSNPDGAWYLEEVRRQLVDMFSEENCKRNGYDFGIYGEDAVYEL
GLTVHTAMDPVQQKAANDGLRHGLEDATKRHGWLGPVKHLEPSEYTEFLQKKNFELADLD
DDGWAQALVTAVNKSGADVMLSEDQRGHVAVSTMNWARKPNKRVAGSVTATVIRDATKVV
KVGDVIWVSRDNTPKKNAKNAKTADASKVIPLRLQQYPDVQGDMVSIEPKRGDVVAMVGG
YAFGSGGSQFNRVTQAQRQPGSSFKPIVYSAALDNGFTPASMLLDAPIILIDKWTKEAWR
PRNDDGKFDGPMPLYRALARSRNLCTVRVAQQMGIEHVIARAKALRLAPDFPATLAISLG
AVAVSPLNLAQAYTAFANEGKVSSPRFITSIQGPWGNTIYECPPENVQAITPQNAYVMAK
LLEGVVNFGTGGKARVLGRPIGGKTGTTNDENDAWFIGVTPHLVTATYIGYDQLQSMGRG
ETGSGAALPAYIYYAKEALKAYPADDFPQPEGIVFADAGGGVSMPFIEGTQPGTGYGLDA
LDGGLDPEAAAQAAEQAQQGEDLLKEMF

Enzyme Prediction     

No EC number prediction in MGYG000003924_00718.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	116	292	1.1e-68	0.9943502824858758

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG5009	MrcA	0.0	64	847	3	779	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	0.0	127	800	1	530	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	1.18e-180	58	836	4	638	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
PRK11636	mrcA	4.08e-148	64	832	2	828	penicillin-binding protein 1a; Provisional
pfam00912	Transgly	2.54e-81	116	292	1	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AAS95114.1	7.51e-305	63	868	2	807
ABM29342.1	7.51e-305	63	868	2	807
ADP85746.1	7.51e-305	63	868	2	807
AGC49761.1	1.22e-287	59	868	2	804
CAJ54403.1	1.22e-287	59	868	2	804

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4OON_A	1.77e-138	112	814	23	738	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3UDF_A	7.79e-126	112	804	23	721	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
5U2G_A	3.97e-97	93	836	5	804	2.6Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20],5U2G_B 2.6 Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20]
7U4H_A	1.62e-64	110	772	21	736	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]
3NB6_A	1.55e-54	117	308	9	200	Crystalstructure of Aquifex aeolicus peptidoglycan glycosyltransferase in complex with Methylphosphoryl Neryl Moenomycin [Aquifex aeolicus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q07806	2.53e-143	64	814	5	765	Penicillin-binding protein 1A OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=mrcA PE=1 SV=2
O87626	2.80e-142	95	814	35	720	Penicillin-binding protein 1A OS=Neisseria flavescens OX=484 GN=mrcA PE=3 SV=1
O05131	4.96e-139	60	855	11	756	Penicillin-binding protein 1A OS=Neisseria gonorrhoeae OX=485 GN=mrcA PE=1 SV=3
Q5FAC7	4.96e-139	60	855	11	756	Penicillin-binding protein 1A OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) OX=242231 GN=mrcA PE=3 SV=3
O87579	7.51e-138	95	855	35	756	Penicillin-binding protein 1A OS=Neisseria lactamica OX=486 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999887	0.000080	0.000021	0.000001	0.000000	0.000014

TMHMM  Annotations     

start	end
65	87