CAZyme Information: MGYG000003911_00258

Basic Information

• Species: QAMM01 sp900762715
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; QAMM01; QAMM01 sp900762715
• CAZyme ID: MGYG000003911_00258
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
645	MGYG000003911_18|CGC1	73127.89	5.2103

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003911	1894498	MAG	China	Asia

• Gene Location: Start: 1182; End: 3119 Strand: +

Full Sequence      

MQKRLQSLPLKNIAIHDNYWSRYINTVPAGLSYQWDALNDRLPDAEKSYCMHNFKVAAGL
EEGEFGGRCFQDSDVAKWLEGVAYYLETNRDPELEKIADEAIEIIGKAQQDDGYLDTYFI
VAEPDKRWTNLRDWHELYCAGHFIEAAVAYYNSTGKDRLLKIICRYVDLICNTIGKEEGK
IHGYPGHPEIELALVKLYRVTGEKRYLDLAKYFIDERGQQPVYFDMEKSRSDPQAAVDPQ
IFNHTYYQVHAPIREQDTAEGHAVRNAYLYTGVADVACETGDETLLKTCETIFDNIAEKR
MYITGSIGSAANGERFSTDYDLPNHSSYSESCASIALAMFAKRMLEIKRDSKYADVMENA
LYNTVLAGISLEGNRFFYVNPLEVVPDVDEKNLDLRHVKTERQKWFGCACCPPNIIRTLA
SLGQYIYSADDENLFVNLYVSNETHCKIGGRDVTVSVRTQYPYGSGIEIKVKTDSDDPFT
LALRLPKHTFLNRMTVDGAPAHEVLEKGYLKLKRNWKGETVVSILLDIPVLRIYANPKVR
ADIGRVALVKGPVVYCLEEADNGANLGGFELPDDAALREEYDNTLFGGTMTITAEGVKVV
DDGNASLYRTTPPKKEKAVMKFVPYCYWNNRGKGEMLVWVCREIK

Enzyme Prediction     

EC	3.2.1.185

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	14	556	7e-218	0.9961832061068703

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	0.0	5	643	9	588	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	5.75e-168	17	556	6	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
cd04792	LanM-like	3.12e-07	81	211	550	677	Cyclases involved in the biosynthesis of class II lantibiotics, and similar proteins. LanM-like proteins. LanM is a bifunctional enzyme, involved in the synthesis of class II lantibiotics. It is responsible for both the dehydration and the cyclization of the precursor-peptide during lantibiotic synthesis. The C-terminal domain shows similarity to LanC, the cyclase component of the lan operon, but the N terminus seems to be unrelated to the dehydratase, LanB.
COG4225	YesR	0.006	123	216	29	114	Rhamnogalacturonyl hydrolase YesR [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADH61394.1	4.03e-239	4	640	8	645
AGF54727.1	9.29e-239	1	644	11	662
AQR93686.1	1.32e-238	1	644	11	662
QAA32625.1	6.33e-237	3	645	7	652
AGB41651.1	6.79e-235	31	640	30	643

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	1.04e-235	12	640	15	645	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	2.07e-230	12	640	15	645	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	2.65e-158	12	638	2	654	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	3.73e-158	12	638	2	654	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]
7EXU_A	1.05e-157	12	638	2	654	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	1.45e-157	12	638	2	654	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000064	0.000004	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003911_00258.