CAZyme Information: MGYG000003844_00959

Basic Information

• Species: Ruminiclostridium_E sp900539195
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Ruminiclostridium_E; Ruminiclostridium_E sp900539195
• CAZyme ID: MGYG000003844_00959
• CAZy Family: GH5
• CAZyme Description: Endoglucanase A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
419	MGYG000003844_4|CGC1	47230.65	4.2447

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003844	2672388	MAG	United States	North America

• Gene Location: Start: 105866; End: 107125 Strand: -

Full Sequence      

MKKRLTAILTAVALMLTGCAGNVTSGTESQPASDVQSGISDNESSDTASVPENDASRYEI
ITATEMAEKMGLGISLGNTMEAYESTDCEKMSYEWIPVVGGNEPQDYETCWGADVTTQEI
IDGMKAEGFNTVRIPVFWGNMMENDGTYTINKEYLKRVKEIVDYCEKAGVYSVINIHHFD
EFIIRRNDLDKCKEIFTHLWTQIAEYFADYPYTLVFEGYNEYLGGDQFDSNGNLKAQSET
NAYKMTNTLNQAFVDAVRSTGGYNAQRVLIVSGYWTNIDKTTSARFTMPEDLVNDRLMVS
VHYVDNSMYWANKIGGEEWLKYIDSQCAELKDAFLDNDIPVFMGETTSTYSESNFAKDAE
YTDSSECLSIVLGKLTDLGIVPVIWDTNSHFYSRTTYKIVNSSDRKVIREYADKLKAEQ

Enzyme Prediction     

No EC number prediction in MGYG000003844_00959.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH5	107	388	7.7e-83	0.9927536231884058

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00150	Cellulase	4.90e-48	107	390	15	271	Cellulase (glycosyl hydrolase family 5).
COG2730	BglC	6.70e-15	107	354	66	329	Aryl-phospho-beta-D-glucosidase BglC, GH1 family [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBK97722.1	2.54e-285	1	417	1	410
CBL35246.1	7.29e-285	1	417	1	410
AEY66181.1	4.83e-74	62	399	39	368
CCO05195.1	2.48e-59	53	410	43	395
AWI66941.1	1.13e-57	55	398	13	346

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6XRK_A	7.79e-49	62	398	23	357	GH5-4broad specificity endoglucanase from an uncultured bovine rumen ciliate [uncultured bovine rumen ciliate],6XRK_B GH5-4 broad specificity endoglucanase from an uncultured bovine rumen ciliate [uncultured bovine rumen ciliate]
4W85_A	4.02e-45	65	403	7	322	Crystalstructure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from ruminal metagenomic library, in complex with glucose [uncultured bacterium],4W85_B Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from ruminal metagenomic library, in complex with glucose [uncultured bacterium],4W87_A Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from metagenomic library, in complex with a xyloglucan oligosaccharide [uncultured bacterium],4W87_B Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from metagenomic library, in complex with a xyloglucan oligosaccharide [uncultured bacterium],4W89_A Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from metagenomic library, in complex with cellotriose [uncultured bacterium],4W89_B Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from metagenomic library, in complex with cellotriose [uncultured bacterium]
6WQY_A	4.25e-45	62	401	23	361	ChainA, Cellulase [Phocaeicola salanitronis DSM 18170]
4W86_A	1.14e-43	65	403	7	322	Crystalstructure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from ruminal metagenomic library, in complex with glucose and TRIS [uncultured bacterium],4W86_B Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from ruminal metagenomic library, in complex with glucose and TRIS [uncultured bacterium]
4W84_A	1.16e-43	65	403	7	322	Crystalstructure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from ruminal metagenomic library, in the native form [uncultured bacterium],4W84_B Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from ruminal metagenomic library, in the native form [uncultured bacterium],4W88_A Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from ruminal metagenomic library, in complex with a xyloglucan oligosaccharide and TRIS [uncultured bacterium],4W88_B Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from ruminal metagenomic library, in complex with a xyloglucan oligosaccharide and TRIS [uncultured bacterium]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q12647	3.52e-42	61	408	23	351	Endoglucanase B OS=Neocallimastix patriciarum OX=4758 GN=CELB PE=2 SV=1
P20847	5.44e-41	62	388	38	358	Endoglucanase 1 OS=Butyrivibrio fibrisolvens OX=831 GN=end1 PE=3 SV=1
P17901	9.02e-39	50	389	42	369	Endoglucanase A OS=Ruminiclostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) OX=394503 GN=celCCA PE=1 SV=1
O08342	9.07e-39	61	386	38	367	Endoglucanase A OS=Paenibacillus barcinonensis OX=198119 GN=celA PE=1 SV=1
A7LXT7	2.56e-38	61	396	148	485	Xyloglucan-specific endo-beta-1,4-glucanase BoGH5A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02653 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000000	0.000000	1.000050	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003844_00959.