CAZyme Information: MGYG000003808_00367

Basic Information

• Species: Pauljensenia culturomici
• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Actinomycetales; Actinomycetaceae; Pauljensenia; Pauljensenia culturomici
• CAZyme ID: MGYG000003808_00367
• CAZy Family: GH25
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
580	MGYG000003808_10|CGC1	62024.75	4.6284

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003808	2368539	MAG	Canada	North America

• Gene Location: Start: 34085; End: 35827 Strand: +

Full Sequence      

MRNARAKIVSAALCAVAAFALANPSASAADPVVESLPATAAEQAGAPELPEVVSPSIADD
DLLVSDDLVMTPEGDVKDVRTGEVVTDPAIVGTPDAPPDPLAKTDGRRFIPVEVGELRAA
ESSGTGETSDGSGAGSQSSQDARESGAASAAPSASRNLVGADGAFAASPAAFSSSPSAYG
AYWGTYLGEPAFFDGKGRLFAQQATTVIDVSQWQGTIDWSAVKRSGVDAAIIRLGYSDTG
IDTKVMTNISECKRLGIPFGVYLFSYAENATDGANEAKHVLALLRQAGVKPSDLAYPVFY
DLERWSYTGASSPTTTKAYESIVGAWWSTMTAAGYERLSLYSYTDMLKNELNSSSLHAKT
MWAASYGATVDYAYPANYRGWQYTSEQRVQGITENVVDVSAFGNGEWTTDVNLTWIYREE
DIAVGAALADSYAGPVEYRFSQYDVAAGKWRTFADWTGANWAGWASTAGTYWLHVEARDA
STHKTLGTQTIAFAYKAGTTRITGTFAGWRSDGILLGAVSNNPKATTRIKIYDTATKQWV
ADFKGPWALWHPKKGVYWTHFEVYTSDGRLAQTLTYAFGV

Enzyme Prediction     

No EC number prediction in MGYG000003808_00367.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH25	208	392	1.6e-39	0.9943502824858758

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06414	GH25_LytC-like	2.27e-61	207	402	3	190	The LytC lysozyme of Streptococcus pneumoniae is a bacterial cell wall hydrolase that cleaves the beta1-4-glycosydic bond located between the N-acetylmuramoyl-N-glucosaminyl residues of the cell wall polysaccharide chains. LytC is composed of a C-terminal glycosyl hydrolase family 25 (GH25) domain and an N-terminal choline-binding module (CBM) consisting of eleven homologous repeats that specifically recognizes the choline residues of pneumococcal lipoteichoic and teichoic acids. This domain arrangement is the reverse of the major pneumococcal autolysin, LytA, and the CPL-1-like lytic enzymes of the pneumococcal bacteriophages, in which the CBM (consisting of six repeats) is at the C-terminus. This model represents the C-terminal catalytic domain of the LytC-like enzymes.
cd00599	GH25_muramidase	3.23e-28	207	402	2	186	Endo-N-acetylmuramidases (muramidases) are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. This family of muramidases contains a glycosyl hydrolase family 25 (GH25) catalytic domain and is found in bacteria, fungi, slime molds, round worms, protozoans and bacteriophages. The bacteriophage members are referred to as endolysins which are involved in lysing the host cell at the end of the replication cycle to allow release of mature phage particles. Endolysins are typically modular enzymes consisting of a catalytically active domain that hydrolyzes the peptidoglycan cell wall and a cell wall-binding domain that anchors the protein to the cell wall. Endolysins generally have narrow substrate specificities with either intra-species or intra-genus bacteriolytic activity.
cd06523	GH25_PlyB-like	1.02e-25	207	396	2	172	PlyB is a bacteriophage endolysin that displays potent lytic activity toward Bacillus anthracis. PlyB has an N-terminal glycosyl hydrolase family 25 (GH25) catalytic domain and a C-terminal bacterial SH3-like domain, SH3b. Both domains are required for effective catalytic activity. Endolysins are produced by bacteriophages at the end of their life cycle and participate in lysing the bacterial cell in order to release the newly formed progeny. Endolysins (also referred to as endo-N-acetylmuramidases or peptidoglycan hydrolases) degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues.
pfam01183	Glyco_hydro_25	1.64e-21	208	392	1	180	Glycosyl hydrolases family 25.
cd06522	GH25_AtlA-like	6.54e-20	206	400	2	189	AtlA is an autolysin found in Gram-positive lactic acid bacteria that degrades bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. This family includes the AtlA and Aml autolysins from Streptococcus mutans which have a C-terminal glycosyl hydrolase family 25 (GH25) catalytic domain as well as six tandem N-terminal repeats of the GBS (group B Streptococcus) Bsp-like peptidoglycan-binding domain. Other members of this family have one or more C-terminal peptidoglycan-binding domain(s) (SH3 or LysM) in addition to the GH25 domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QOL28163.1	1.37e-197	48	580	103	608
QOL29751.1	1.37e-197	48	580	103	608
QSG87589.1	3.08e-197	48	580	127	632
QXT32014.1	3.08e-197	48	580	127	632
SDO33591.1	6.10e-195	48	580	129	634

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2WW5_A	9.79e-38	188	402	251	467	3D-structureof the modular autolysin LytC from Streptococcus pneumoniae at 1.6 A resolution [Streptococcus pneumoniae R6],2WWD_A 3D-structure of the modular autolysin LytC from Streptococcus pneumoniae in complex with pneummococcal peptidoglycan fragment [Streptococcus pneumoniae R6]
2WWC_A	2.51e-37	188	402	251	467	3D-structureof the modular autolysin LytC from Streptococcus pneumoniae in complex with synthetic peptidoglycan ligand [Streptococcus pneumoniae R6]
1JFX_A	2.78e-08	208	396	8	197	Crystalstructure of the bacterial lysozyme from Streptomyces coelicolor at 1.65 A resolution [Streptomyces coelicolor]
3HMC_A	1.78e-06	207	392	7	171	Endolysinfrom Bacillus anthracis [Bacillus anthracis]
4KRU_A	2.76e-06	208	392	23	198	X-raystructure of catalytic domain of endolysin from clostridium perfringens phage phiSM101 [Clostridium phage phiSM101]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P26836	1.85e-08	208	464	12	255	Probable autolytic lysozyme OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=lyc PE=3 SV=2
P25310	3.27e-07	208	396	85	274	Lysozyme M1 OS=Streptomyces globisporus OX=1908 GN=acm PE=1 SV=1
P34020	5.23e-07	208	405	4	183	Autolytic lysozyme OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=lyc PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000406	0.998678	0.000232	0.000242	0.000226	0.000183

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003808_00367.