CAZyme Information: MGYG000003805_01354

Basic Information

• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Mycobacteriales; Mycobacteriaceae; Corynebacterium
• CAZyme ID: MGYG000003805_01354
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
889		94798.56	4.5479

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003805	2110817	MAG	Canada	North America

• Gene Location: Start: 697; End: 3366 Strand: +

Full Sequence      

MQVRRVTDKKNGHNASPKDPNSKPRPQETSSDPQEAQQAAAESQQDPAKGTAARKAEAKP
TAGQPAAKPKAEKESSEPKAKPKKAKPQQPTPEKQPAKAANSTDGAQQDVKSAQKPQPAA
NAAGSAAVGADATEPAPAGEPPQRIPEEQVATENERVSPIMGGSAAVSKIRSHRFGQWIF
AIVLLLLLLAAIPLIWFFQAYAKAEIPEPGEIETAQVSNIYFSDAETELARVVPPEGNRE
QVPLEQVPTHVQNAVLAAEDRDFWKNSGFSFSGFMRAVIGQITGNVSAGGGSTVTQQYVK
NVLVGNEHSYERKARELVYSVKMTNEWSKEDILTAYLNTVYFGRNAYGVEAAAHAFFGKP
VSELTLEEGAVLAASIQLPSQLDPWTNPDGATARWDYVMDGLVDMGEISKEERQNAVYPE
TRDPASYSAYTEATGPNGLIKNQVMLELETIGITEDDLTKRGLQITTTIDADTQAEVERI
AADQLKPLQDDARSAVVGIEPATGAVRAYYGGDDPSGWDYANAGLQTGSTFKIFALAAAL
QQGIPLHAVYDSSPVTLPGNFTITNWDGGGGGMISVSEAVKTSSNTAFMRIQDDLDNTTQ
DTADMAHALGVARSIPGIPVTLRENGEQPYPGIVLGQYQTRVLDMATAMATLTDRGVYHP
TYFVEKIVDAKGELLYEVNHDYAERRVSEQVADNVIEAMKSVVGYSNGTLEGGRPSAGKT
GTAQMGDTGQNKDAWMVGGTPELSLAVWVGTADNTSPIFNEWGGNMFGANAPTQIWKKVL
DSALKGQEFKDFPPAQPVSWGINPYHGGVNLGGVPGPQPGSAPPPPAGEPNGAHPEGHEA
PAPQPHPEEAHPAPEAVPVPEPAPVLEPEPESLQDAIDGMIGNLNRVLP

Enzyme Prediction     

No EC number prediction in MGYG000003805_01354.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	236	402	5.9e-62	0.9378531073446328

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	6.85e-161	197	808	34	624	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	1.30e-127	238	786	1	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG5009	MrcA	7.00e-83	201	880	29	796	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
pfam00912	Transgly	1.41e-73	238	403	12	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.
COG4953	PbpC	4.27e-65	206	814	27	592	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QYH20513.1	0.0	188	814	1	627
AQQ16316.1	0.0	181	813	95	728
QNP94064.1	0.0	162	813	26	677
QQU84458.1	0.0	174	813	34	673
AKV59474.1	0.0	174	813	15	654

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5CXW_A	5.74e-107	435	793	2	365	Structureof the PonA1 protein from Mycobacterium Tuberculosis in complex with penicillin V [Mycobacterium tuberculosis H37Rv]
5CRF_A	9.65e-104	435	793	2	365	Structureof the penicillin-binding protein PonA1 from Mycobacterium Tuberculosis [Mycobacterium tuberculosis H37Rv],5CRF_B Structure of the penicillin-binding protein PonA1 from Mycobacterium Tuberculosis [Mycobacterium tuberculosis H37Rv],5CRF_C Structure of the penicillin-binding protein PonA1 from Mycobacterium Tuberculosis [Mycobacterium tuberculosis H37Rv],5CRF_D Structure of the penicillin-binding protein PonA1 from Mycobacterium Tuberculosis [Mycobacterium tuberculosis H37Rv]
3ZG8_B	4.30e-43	327	792	7	469	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
3UDF_A	7.19e-43	231	786	29	718	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
5FGZ_A	3.51e-40	229	780	145	690	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A0R7G2	2.74e-203	192	830	85	718	Penicillin-binding protein 1A OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=ponA1 PE=3 SV=1
P71707	3.63e-195	192	793	147	755	Penicillin-binding protein 1A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ponA1 PE=1 SV=3
P38050	8.67e-53	238	783	66	597	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2
Q8DNB6	8.80e-49	201	750	74	622	Penicillin-binding protein 2a OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=pbp2a PE=1 SV=1
A0A0H2ZMF9	8.80e-49	201	750	74	622	Penicillin-binding protein 2a OS=Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) OX=373153 GN=pbp2a PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000082	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
178	200