CAZyme Information: MGYG000003794_01118

Basic Information

• Species: Prevotella timonensis
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella timonensis
• CAZyme ID: MGYG000003794_01118
• CAZy Family: GH85
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1018	MGYG000003794_46|CGC1	113007.46	6.4724

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003794	2491297	MAG	Canada	North America

• Gene Location: Start: 7044; End: 10100 Strand: +

Full Sequence      

MKKRFLPLMLLACLAGPANAQSGMSPTATSTIYDFAGFNEVTLLTLFDRALQRGRNYPTK
EEFEAAGIQASDIAFVRSHVKQRAILDRKDRLVKDTYEKRNLFMNIPSGSGKGIGGYPSS
NFANDVFSMWNYTNLFGSWNHGLFQAPGAWTDAAHKNGTDIMSGIKFFESWTAGSRDKDY
VRFLSTKDGNGHYQYVKPMLNCLIFFGFDGINYNWEDNSYSEPDVTAFHKELYKEAEKIG
FNNFHAAIYTLGSSLSSAAVDYLYGTKETGKTFDLFLNYSSGDFATSGYESSVKAAEKAM
GTAEGLYGGAWIVSMNRNWLGLVGTINYDWNTYQQIFTPNETVKRIGICLWGEHGDSRFW
SYNSGSDALNAQYNYQRLLERGFSGGNRNPFNRPAITNSGHEWEGENALKNFHGLASFIP
ERSAIQGNLPFLTHFNLGNGERYNYKGKKTAGTWYNMANQDVVPTYRWLVYGEGTETVSN
ALQPEFTHIDSYNGGSCLQLRGLSSASGVDVVLFRTDLNVSAANPIAKVALKTKAAGESN
LYLIVKVNNQWKEVAVGATDDKTWQEKKVSLPVNMGDRITHIGLRAKNVTDKYNVLVGKL
ELNDDVKATPANVKNLMVEVKEESQKSLSAKLYWDVDHTTGERSAYGMIYNCEANIDHFE
IMYKNGKDGRISEVGRTTQWGTYVPNIVFADANEKPFIGVRSVSTDLKTYSPIEWVEITR
ADASQLPEETVGGTDTYGISKINPNADGFKTAVEQRFLTSVTTTGAVKDLNYTATESPRD
SSQYANARDHKLVVKQGQQITMTFKAPNEKDGLKWCFAGGWLDLDGSGTFNYPKAPKDDP
MGEQIFFAGKIRAATPEIQDPNGYTFKFTVPQDAHVGTSRLRIVFADAWFGGTFLPTGLT
AKGFTIDFDVEIQGEKNGQRQYVDTQHDQGIADQPEGLSGTDGVDNTVFAGGVSNVTVNG
NDILFTNVEKAWIYTVDGKIVQYLTNPTSLSRSELQGGVYLVKMQNKNVIRTEKLVIK

Enzyme Prediction     

No EC number prediction in MGYG000003794_01118.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH85	120	443	2.8e-40	0.9492063492063492

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4724	COG4724	1.40e-27	72	605	49	552	Endo-beta-N-acetylglucosaminidase D [Carbohydrate transport and metabolism].
cd06547	GH85_ENGase	5.30e-21	115	474	8	339	Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the protein and N-acetylglucosamine 2 forms the reducing end of the released glycan. ENGase is a key enzyme in the processing of free oligosaccharides in the cytosol of eukaryotes. Oligosaccharides formed in the lumen of the endoplasmic reticulum are transported into the cytosol where they are catabolized by cytosolic ENGases and other enzymes, possibly to maximize the reutilization of the component sugars. ENGases have an eight-stranded alpha/beta barrel topology and are classified as a family 85 glycosyl hydrolase (GH85) domain. The GH85 ENGases are sequence-similar to the family 18 glycosyl hydrolases, also known as GH18 chitinases. An ENGase-like protein is also found in bacteria and is included in this alignment model.
pfam03644	Glyco_hydro_85	2.20e-09	118	440	1	291	Glycosyl hydrolase family 85. Family of endo-beta-N-acetylglucosaminidases. These enzymes work on a broad spectrum of substrates.
TIGR04183	Por_Secre_tail	9.53e-06	954	1018	6	72	Por secretion system C-terminal sorting domain. Species that include Porphyromonas gingivalis, Fibrobacter succinogenes, Flavobacterium johnsoniae, Cytophaga hutchinsonii, Gramella forsetii, Prevotella intermedia, and Salinibacter ruber average twenty or more copies of a C-terminal domain, represented by this model, associated with sorting to the outer membrane and covalent modification.
pfam18962	Por_Secre_tail	2.91e-04	973	1017	23	72	Secretion system C-terminal sorting domain. Species that include Porphyromonas gingivalis, Fibrobacter succinogenes, Flavobacterium johnsoniae, Cytophaga hutchinsonii, Gramella forsetii, Prevotella intermedia, and Salinibacter ruber have on average twenty or more copies of this C-terminal domain, associated with sorting to the outer membrane and covalent modification. This domain targets proteins to type IX secretion systems and is secreted then cleaved off by a C-terminal signal peptidease. Based on similarity to other families it is likely that this domain adopts an immunoglobulin like fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCI64641.1	0.0	1	1016	1	1005
AGB28382.1	0.0	25	1018	199	1190
QUB72357.1	0.0	10	1018	15	1011
QUT74553.1	0.0	1	1015	1	1017
BBA29895.1	0.0	20	1018	25	1012

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3FHQ_A	5.93e-20	72	670	26	570	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_F Chain F, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]
3FHA_A	7.83e-20	72	670	26	570	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_C Chain C, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]
2VTF_A	7.95e-20	108	670	64	575	X-raycrystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae],2VTF_B X-ray crystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000788	0.853288	0.144903	0.000416	0.000313	0.000261

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003794_01118.