CAZyme Information: MGYG000003769_00658

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lachnoclostridium
• CAZyme ID: MGYG000003769_00658
• CAZy Family: GH48
• CAZyme Description: Exoglucanase-2

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
908	MGYG000003769_4|CGC3	101198.97	4.372

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003769	4856301	MAG	Canada	North America

• Gene Location: Start: 83018; End: 85744 Strand: -

Full Sequence      

MRKHIRRKVTAAVAVVTILFSLLPKTGQVTALADATDAYQQRFLDLWGDLHDPNNGYFSD
QGIPYHSVETLIVEAPDYGHVTTSEALSYYMWLEAMYGKFTGDFTGFETAWDVTEKYLIP
TEEDQPETSMTKYTASKPATYAPEWQDTSKYPAVLDFTAAVGVDPIHNDLVSTYGTSRIY
GMHWLLDVDNWYGFGSRGDGTSKPSYINTFQRGEEESTWETIPQPSWDDMNFGGENGFLD
LFTGDKTYAAQFKYTNAPDADARAVQATYWAQEWAADYGVNIDTYINKATKMGDYLRYSM
FDKYFRKIGNSTAAGTGYDSAHYLLSWYYAWGGGIDSAWAWKIGSSHNHFGYQNPMAAWI
LSKEQDFKPASQNGQADWAKSLDRQLEFYQWLQSAEGAIAGGASNSNNGRYEAWPAGTST
FYGMGYVENPVYADPGSNTWFGFQAWSMQRVAELYYQSGDVRAKNILEKWVAWIKTVVDL
NADGTFAIPSNIDWTGQPDTWTGTYTGNPNLHVTVTSKGTDLGIAASLANTLLYYAKASG
DDEARVLAKELLDRMWNLYRDDKGLSAPEARADYKRFFEQEVYVPSTFNGTMPNGDALVA
GVKFLDIRSKYLSDPDYQKVLDAYNAGTAPEFNYHRFWAQCDIAIANGLYSILFEENTEP
VVNAAINPTQAEFDKASGKETNITVDMTLNGYTLNGILNGNTPLVSGADYTVTDSTVVIS
KDYLGLQPVGKVQLTFDFSGGIDRVLTVTVVDTTVVELGDIQVQMFNGNTSSTTNGIAPR
IKLYNNGDTVVKLLDVKLRYYYTIDGEIDQNFWCDWTTVGSSNVTGKFEKMSTPKAGADY
YLEIGFTSGAGDLAAGQSIEVQVRFSKTNWSNYTQTGDYSFNSTNNSYADWNKITGYIGD
KLEWGVEP

Enzyme Prediction     

EC	3.2.1.176	3.2.1.-	3.2.1.14

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH48	39	652	2e-290	0.9967266775777414
CBM3	764	845	3e-29	0.9886363636363636

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam02011	Glyco_hydro_48	0.0	39	654	1	620	Glycosyl hydrolase family 48. Members of this family are endoglucanase EC:3.2.1.4 and exoglucanase EC:3.2.1.91 enzymes that cleave cellulose or related substrate.
pfam03442	CBM_X2	3.60e-28	666	743	1	78	Carbohydrate binding domain X2. This domain binds to cellulose and to bacterial cell walls. It is found in glycosyl hydrolases and in scaffolding proteins of cellulosomes (multiprotein glycosyl hydrolase complexes). In the cellulosome it may aid cellulose degradation by anchoring the cellulosome to the bacterial cell wall and by binding it to its substrate. This domain has an Ig-like fold.
pfam00942	CBM_3	7.80e-27	763	844	1	82	Cellulose binding domain.
smart01067	CBM_3	4.00e-24	763	845	1	83	Cellulose binding domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ABX43721.1	0.0	1	908	1	919
BCJ93766.1	0.0	35	908	64	937
BCN30512.1	0.0	1	908	2	916
CUH92782.1	0.0	1	908	1	918
ANW99180.1	0.0	6	908	2	914

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1F9O_A	0.0	31	656	1	629	Crystalstructure of the cellulase Cel48F from C. Cellulolyticum with the thiooligosaccharide inhibitor PIPS-IG3 [Ruminiclostridium cellulolyticum],1FAE_A Crystal structure of the cellulase CEL48F from C. cellulolyticum in complex with cellobiose [Ruminiclostridium cellulolyticum],1FBO_A Crystal structure of the cellulase CEL48F from C. cellulolyticum in complex with cellobiitol [Ruminiclostridium cellulolyticum],1FCE_A PROCESSIVE ENDOCELLULASE CELF OF CLOSTRIDIUM CELLULOLYTICUM [Ruminiclostridium cellulolyticum],1G9G_A Xtal-Structure Of The Free Native Cellulase Cel48f [Ruminiclostridium cellulolyticum]
4EL8_A	0.0	39	654	9	631	Theunliganded structure of C.bescii CelA GH48 module [Caldicellulosiruptor bescii DSM 6725]
1F9D_A	0.0	31	656	1	629	ChainA, ENDO-1,4-BETA-GLUCANASE F [Ruminiclostridium cellulolyticum],1FBW_A Chain A, ENDO-1,4-BETA-GLUCANASE F [Ruminiclostridium cellulolyticum],2QNO_A Chain A, Endoglucanase F [Ruminiclostridium cellulolyticum]
4L0G_A	0.0	39	661	13	642	CrystalStructure of a GH48 cellobiohydrolase from Caldicellulosiruptor bescii [Caldicellulosiruptor bescii DSM 6725],4L6X_A Crystal Structure of a GH48 cellobiohydrolase from Caldicellulosiruptor bescii [Caldicellulosiruptor bescii DSM 6725],4TXT_A Crystal Structure of a GH48 cellobiohydrolase from Caldicellulosiruptor bescii [Caldicellulosiruptor bescii DSM 6725]
6D5D_A	0.0	39	654	20	642	Structureof Caldicellulosiruptor danielii GH48 module of glycoside hydrolase WP_045175321 [Caldicellulosiruptor danielii]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P37698	0.0	1	702	1	695	Endoglucanase F OS=Ruminiclostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) OX=394503 GN=celCCF PE=1 SV=2
P50900	0.0	6	908	2	914	Exoglucanase-2 OS=Thermoclostridium stercorarium (strain ATCC 35414 / DSM 8532 / NCIMB 11754) OX=1121335 GN=celY PE=1 SV=2
P22534	5.81e-319	39	654	1117	1739	Endoglucanase A OS=Caldicellulosiruptor saccharolyticus OX=44001 GN=celA PE=3 SV=2
P0C2S5	4.05e-286	7	702	5	707	Cellulose 1,4-beta-cellobiosidase (reducing end) CelS OS=Acetivibrio thermocellus OX=1515 GN=celS PE=1 SV=1
A3DH67	4.05e-286	7	702	5	707	Cellulose 1,4-beta-cellobiosidase (reducing end) CelS OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=celS PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000277	0.998941	0.000214	0.000207	0.000171	0.000141

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003769_00658.