CAZyme Information: MGYG000003543.1_00152

Basic Information

• Species: HGM04593 sp900769465
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; HGM04593; HGM04593 sp900769465
• CAZyme ID: MGYG000003543.1_00152
• CAZy Family: GH85
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1268		140938.47	4.7484

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003543.1	3216165	MAG	Fiji	Oceania

• Gene Location: Start: 21313; End: 25119 Strand: +

Full Sequence      

MKKHLLTIAAAFLSVTAMAQSVPTHAMYVDNGTPDNIVTALSKWEPGKPWNGDASFVDEN
FWISRVPLKNRFRPGDQANDALNDENNKNFCWMAPTGEMTKKWGALPRYNFDGDNFNMWQ
YVDIHSNWTNGFWRVPGAFNDVAHKNGVKTGCTYFIDWGTAVNSTDEPGKTLYELAVTGT
NSYGDKYKYSRKLIQFLKYYGIDGLCFNPEGYWGLQVYNRFIPFLAECHKIAKELGHPFH
VDWYAFVTNNGSLSDNGCKLNSNNQNWFHHKSTNQPVTDVYFLNYNWTEGGLAESAQTAA
SLGRSTFDVYAGFDMQGRGYGKNGNAGWTALMRQPVSVVVWGAHDRNQLYVSSTEGGQSD
YAVQNEYQKKQEMLFSGGNRNVLNMPGLTDANVTSSFSDLKDWHGYATAVIERSTLDELP
FVTRFNLGNGRFFNKEGVTTWDHKWYNYGMQDLLPTWRWWVDNGDGKTVSADAVNLDFCY
DDAWFAGSCLKIHGATQRSDVRLFATKWNVEAADDEFRLVFKPKSAATNLELMVAKENGE
NNFVYIPVAGDIKAGEWNEVVIKASEAGLAAGDVIGCVGLSVKDTNAAYEVLLGEFAFVP
ASFNEQPQTPTITHAEVMKRYYNRADIKLVWSMPTPASRPAEYEGCPVYNEEVGTWYYEI
YVKQEGQETLLTTTTSWAAYVVDATLIPKVDKLQIGVRAVGKDGRAMSDIVWSQEIEEPL
TMIETLTVDKAIIKPGEEFTIGFEDPNHAKATFAIYDALTDKNVAQSAASVLSFTTSLPN
VGSYDVKVRTGNTTVTNRSLILVTPEETGRLPQINDITSDVAEVTGPDQEVNFTADIVTG
TEYEGNPCTVSQSLYMSEPYQFTVDAAVMSEYTNTSFALWFKVEKFEHASLGTLLMTKVN
RNYNNTWTENVWGEMWTAIRPAGYAKNNNIGRDNAENELSLCTDAPYAGYPNYEHNNDVD
ILSNGYSLMPGVWYHVCAVKSGRNLKLYLNGKEIASGSSRGTGPKDWRGAKFYVGGSMTN
LASLTGWVDEVQIWSKALTADEVKRAMRGYATAPTNLEGYFTFEETKTDDEGYIYFPNKG
QNVAAVPGGYMTTGKEENSRTTDYKQNQLTTTLGVPMLTGVFPVNYESTKWMIEGARVNA
STATTANATYNSGDGKFPITVVAENSWGSTTKTITDYMVVTSIEDVNAENGYMVYPKPFE
GTANVLFAEDGLFEVAVFAADGKLVANDAFDVRAGELREISLASAQSGVYVVVIMKEGVA
VRSFKIKK

Enzyme Prediction     

No EC number prediction in MGYG000003543.1_00152.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH85	110	433	2.1e-58	0.946031746031746

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06547	GH85_ENGase	6.76e-46	111	465	22	339	Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the protein and N-acetylglucosamine 2 forms the reducing end of the released glycan. ENGase is a key enzyme in the processing of free oligosaccharides in the cytosol of eukaryotes. Oligosaccharides formed in the lumen of the endoplasmic reticulum are transported into the cytosol where they are catabolized by cytosolic ENGases and other enzymes, possibly to maximize the reutilization of the component sugars. ENGases have an eight-stranded alpha/beta barrel topology and are classified as a family 85 glycosyl hydrolase (GH85) domain. The GH85 ENGases are sequence-similar to the family 18 glycosyl hydrolases, also known as GH18 chitinases. An ENGase-like protein is also found in bacteria and is included in this alignment model.
COG4724	COG4724	1.09e-44	41	596	39	547	Endo-beta-N-acetylglucosaminidase D [Carbohydrate transport and metabolism].
pfam03644	Glyco_hydro_85	8.68e-40	110	430	4	291	Glycosyl hydrolase family 85. Family of endo-beta-N-acetylglucosaminidases. These enzymes work on a broad spectrum of substrates.
pfam13385	Laminin_G_3	2.21e-17	956	1044	64	151	Concanavalin A-like lectin/glucanases superfamily. This domain belongs to the Concanavalin A-like lectin/glucanases superfamily.
smart00560	LamGL	4.95e-06	971	1041	61	133	LamG-like jellyroll fold domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT77741.1	0.0	3	1268	4	1277
BCI64756.1	0.0	3	1266	4	1280
QUB76736.1	7.88e-250	1	1268	1	1258
QUB42054.1	3.89e-249	1	1268	1	1254
EFC71170.1	1.32e-248	1	1268	1	1249

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2W91_A	6.21e-40	41	704	20	622	Structureof a Streptococcus pneumoniae family 85 glycoside hydrolase, Endo-D. [Streptococcus pneumoniae TIGR4],2W92_A Structure of a Streptococcus pneumoniae family 85 glycoside hydrolase, Endo-D, in complex with NAG-thiazoline. [Streptococcus pneumoniae TIGR4]
3GDB_A	2.82e-39	41	733	171	809	Crystalstructure of Spr0440 glycoside hydrolase domain, Endo-D from Streptococcus pneumoniae R6 [Streptococcus pneumoniae R6]
3FHQ_A	1.95e-31	41	655	16	575	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_F Chain F, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]
3FHA_A	2.59e-31	41	655	16	575	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_C Chain C, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]
2VTF_A	8.45e-31	41	655	21	580	X-raycrystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae],2VTF_B X-ray crystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000304	0.998931	0.000182	0.000203	0.000177	0.000157

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003543.1_00152.