CAZyme Information: MGYG000003394_00565

Basic Information

• Species: Haemophilus influenzae
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Pasteurellaceae; Haemophilus; Haemophilus influenzae
• CAZyme ID: MGYG000003394_00565
• CAZy Family: GT25
• CAZyme Description: Lipooligosaccharide biosynthesis protein lex-1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
256	MGYG000003394_13|CGC1	29391.82	7.1946

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003394	1902954	MAG	United States	North America

• Gene Location: Start: 49242; End: 50012 Strand: +

Full Sequence      

MHSTVQYNYVISLTTEQKRRKHITEEFGKQNIPFAFFDAITPDIIEETAKKFNITLDRSP
KAKLSDEEIGCALSHIVLWDLALENNLNYINIFEDDIHLGENAKELLEIDYISDDIHILK
LEANGKMFFKQPQSVKCDRNIYPITVKQSGCGGYTVTAKGAKYLLELVKNKPLDVAVDSL
VFGDFLHFKDYKIVQLSPGICVQDVVLHPENHFESSLKEGRDRVHGNQRKFSILEKIKNE
FGRVKIKMFGKQVPFK

Enzyme Prediction     

EC	2.4.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT25	7	180	2.8e-37	0.9779005524861878

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3306	COG3306	3.50e-65	8	247	5	252	Glycosyltransferase involved in LPS biosynthesis, GR25 family [Cell wall/membrane/envelope biogenesis].
pfam01755	Glyco_transf_25	7.84e-52	8	181	4	200	Glycosyltransferase family 25 (LPS biosynthesis protein). Members of this family belong to Glycosyltransferase family 25 This is a family of glycosyltransferases involved in lipopolysaccharide (LPS) biosynthesis. These enzymes catalyze the transfer of various sugars onto the growing LPS chain during its biosynthesis.
cd06532	Glyco_transf_25	4.01e-30	8	178	2	125	Glycosyltransferase family 25 [lipooligosaccharide (LOS) biosynthesis protein] is a family of glycosyltransferases involved in LOS biosynthesis. The members include the beta(1,4) galactosyltransferases: Lgt2 of Moraxella catarrhalis, LgtB and LgtE of Neisseria gonorrhoeae and Lic2A of Haemophilus influenzae. M. catarrhalis Lgt2 catalyzes the addition of galactose (Gal) to the growing chain of LOS on the cell surface. N. gonorrhoeae LgtB and LgtE link Gal-beta(1,4) to GlcNAc (N-acetylglucosamine) and Glc (glucose), respectively. The genes encoding LgtB and LgtE are two genes of a five gene locus involved in the synthesis of gonococcal LOS. LgtE is believed to perform the first step in LOS biosynthesis.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBE81655.1	2.42e-188	1	256	1	256
AXP40639.1	2.42e-188	1	256	1	256
BBE83579.1	2.42e-188	1	256	1	256
AGS36337.1	6.65e-186	1	256	1	256
AGS36331.1	5.47e-185	1	256	1	256

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q57125	3.96e-176	1	256	27	282	Putative glycosyltransferase HI_0765 OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=HI_0765 PE=3 SV=1
Q05770	4.12e-69	8	256	3	263	Lipooligosaccharide biosynthesis protein LpsA OS=Mannheimia haemolytica OX=75985 GN=lpsA PE=3 SV=2
Q03974	3.42e-31	8	245	6	272	Lipooligosaccharide biosynthesis protein lex-1 OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=lex1 PE=1 SV=1
Q57394	4.33e-31	8	211	4	211	Lipooligosaccharide biosynthesis protein lic2B OS=Haemophilus influenzae OX=727 GN=lic2B PE=3 SV=1
Q50950	6.24e-30	8	252	3	263	Lacto-N-neotetraose biosynthesis glycosyltransferase LgtE OS=Neisseria gonorrhoeae OX=485 GN=lgtE PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000038	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003394_00565.