CAZyme Information: MGYG000003359_04653

Basic Information

• Species: Paenibacillus_A sp900766135
• Lineage: Bacteria; Firmicutes; Bacilli; Paenibacillales; Paenibacillaceae; Paenibacillus_A; Paenibacillus_A sp900766135
• CAZyme ID: MGYG000003359_04653
• CAZy Family: GH115
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1262		138699.73	4.829

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003359	5721146	MAG	United States	North America

• Gene Location: Start: 1406; End: 5194 Strand: -

Full Sequence      

MFGPSYIRYAPEEESAALRLVDQGAAADICVSPEDHPGVIRAAGDLQADIERVTGRKPRL
VEALEAGGLSGHAVLIGTIGQSPLIDCLIAEGRLDVSRVAGRWESFVIQTVPSPLPGIEW
ALVIAGSDKRGTIFGIYDVSERIGVSPWHWWADVPPQRRDALYVKPGRYAQGEPSVKYRG
IFLNDEGPSLMTWVRDNFRDFTHEFYEKVYELLLRLKANYLWPAMWDNTFYEDDPLNAEV
ADRYGIVIGTSHHEPMLRPHGDWKLHRKGPWDYSVNGEVLYRFWEEGIRRSQDYESIVTL
GMRGDGDEAMGGHLTFEEKIALLERIVRDQRGMIAREMNKDASKVPQLWALYKEVQDYYE
HGMRVPDDITLLWSDDNHGNLRRVPTGEERGRSGGAGIYYHLDYVGGPRSYKWVNTVPLP
KIWEQMHKAYEYGADRIWILNVGDLKPVELPLEYFLRMAWRISDFTKDNAREFAEAWAER
QFGSAYRKAAADVLTGYTKFNGRVKPELLNAVELYSLTDYKEAETALREFHGIVRTAERL
YAEMPEELRDACFQLLLYPAKASWQVLELHVRAARSRLYAAQGRTAANLEAREAELLFEA
DRELTLHYNQLLAQGKWNHMMDQTHIGYTYWNQPPVNVMPETGRVEVRSGAEMGVAVEGS
TEVWVAPDSCRLPTLDAFTREARYIEVFNRGSDPFAFRMQADVPWLKLSASEGMVVHQKR
IWVDVDWELAPIGERVTGRVDVSGPGDVVVTVEAELFNPASPKREELRGHMETGGVVSIE
AEHFARAVPAANGAAWSRIDGYGRTLSSMAVLPVMVPSVERPGAEPDRSPSLEYPVYLTS
CGVLDVRLYLAPTIDFVPGSGLRIGVQFDDGPVLVADAISHLADGGFDERDWEQSVIYNV
RTAVTRHSIGQPGLHTLRIWMVDPIVALQKIVIDAGGAKPSLLGPPESFWGGAAADVDAV
GGTARDGGTPSGGEVGGDAGTGSDGAGVVQIGSAPLAGQEAGGDAYAGSTGAVQGDAAAI
TMREAVNLGDGYDPFELPGVVDGAFAGLSGEALEVFVRESGLYALELTATAAGPDAPPAA
VSLALDGRDLPGGPFLLAPAAGRAGAVLRCAAERLHLPAGRQVLRLRAEGGRAVPQELRL
TLTEPDRLAVRPSLRRSGPTDLPLIAQIGLLDAGALYSCRYVLVVSLYGEDDQLYGQAAV
TGTVPRAGEDVQRLAFPDREPLQASAVSAPADSCPPPRRYKLKLVLTWEGGQPREYVSEW
LL

Enzyme Prediction     

EC	3.2.1.131

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH115	17	648	2.4e-263	0.8550932568149211

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam15979	Glyco_hydro_115	0.0	172	508	1	334	Glycosyl hydrolase family 115. Glyco_hydro_115 is a family of glycoside hydrolases likely to have the activity of xylan a-1,2-glucuronidase, EC:3.2.1.131, or a-(4-O-methyl)-glucuronidase EC:3.2.1.-.
pfam17829	GH115_C	8.34e-63	769	947	1	172	Gylcosyl hydrolase family 115 C-terminal domain. This domain is found at the C-terminus of glycosyl hydrolase family 115 proteins. This domain has a beta-sandwich fold.
pfam03648	Glyco_hydro_67N	6.31e-06	8	138	4	119	Glycosyl hydrolase family 67 N-terminus. Alpha-glucuronidases, components of an ensemble of enzymes central to the recycling of photosynthetic biomass, remove the alpha-1,2 linked 4-O-methyl glucuronic acid from xylans. This family represents the N-terminal region of alpha-glucuronidase. The N-terminal domain forms a two-layer sandwich, each layer being formed by a beta sheet of five strands. A further two helices form part of the interface with the central, catalytic, module (pfam07488).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AOZ93060.1	0.0	1	1262	1	1191
AZP03538.1	0.0	1	948	1	924
ASG24142.1	0.0	4	948	27	955
QGZ43271.1	0.0	17	948	31	938
ALJ01085.1	0.0	2	948	7	937

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4ZMH_A	3.23e-292	9	952	5	937	Crystalstructure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40],4ZMH_B Crystal structure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40]
4C90_A	6.25e-185	20	659	46	679	Evidencethat GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C90_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_A Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus]
7PUG_A	1.76e-178	3	948	6	834	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]
6NPS_A	6.84e-177	18	950	11	965	Crystalstructure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112],6NPS_B Crystal structure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112]
7PXQ_A	7.12e-177	3	948	5	833	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000040	0.000004	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003359_04653.