CAZyme Information: MGYG000003245_00452

Basic Information

• Species: Mediterraneibacter sp900761655
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Mediterraneibacter; Mediterraneibacter sp900761655
• CAZyme ID: MGYG000003245_00452
• CAZy Family: GH2
• CAZyme Description: Beta-glucuronidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
597	MGYG000003245_62|CGC1	68374.18	5.0495

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003245	2245318	MAG	United States	North America

• Gene Location: Start: 3461; End: 5254 Strand: +

Full Sequence      

MLYPKINDARTVMDLSGIWDFKLDNGKGFDEGWTQKPLSDPHRIAVPASYNDQIEGVDFR
DHYGWAFYQKEIEIPKMLLSQRVVLRFGAVTHKAKVYLNGEMLCEHEGGFLPFEAEINDL
VHPGKNLLCVAVDNRVDYSTLPIGNEVGAAFFGSELPDIPSVNVTVPKPHNAPNFDFFNY
AGINRPVKIYTTPKSYIRDIVLVPQIEGEDAKVRYSVDCCKETEAEVIVHFLDEEGNEVA
CAQGKEGTVVIQNARLWQPGDAYLYTAKVEFGEDLYEEQFGVRSIEIRGTQFLINGRPFY
FKGFGKHEDSEVHGRGINEPMNVKDLSLMKWMGANSFRTSHYPYAEEMLNLCDRLGIVVI
DETSAVGLNFGWNDEGYAKFRTKEHHEQVIRDLIARDKNHPCVAVWSIANEPDTEKQPQA
AHDYFMPLYHLAHECDPQDRPVTLVICQNDYTRDITAPEMDIICANRYYGWYVFSGDLDA
AERAMREEMKYWAKLGKPWMITEYGADTVAGIHMATGNMFSEEYQVDYYKAINKVLDECD
FVVGEHGWNFADFATIQGTLRVDGNKKGVFTRARRPKLAAHYFRQRWHEVPDFDYKK

Enzyme Prediction     

EC	3.2.1.31

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	7	581	1.1e-103	0.6063829787234043

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10150	PRK10150	0.0	1	597	1	602	beta-D-glucuronidase; Provisional
COG3250	LacZ	4.87e-116	10	597	10	617	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam02836	Glyco_hydro_2_C	4.72e-79	285	587	1	297	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK10340	ebgA	7.32e-36	3	413	24	451	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	6.96e-31	15	442	55	485	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AJG98227.1	1.39e-286	1	596	1	598
ACL76021.1	5.94e-282	1	596	1	598
CUO31707.1	3.01e-268	1	596	1	600
AEN95939.1	1.46e-267	1	596	6	605
QNO18226.1	5.32e-266	1	597	1	598

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6BO6_A	8.21e-253	1	596	1	609	Eubacteriumeligens beta-glucuronidase bound to UNC4917 glucuronic acid conjugate [[Eubacterium] eligens ATCC 27750]
6BJQ_A	1.85e-252	1	596	25	633	ChainA, Glycoside Hydrolase Family 2 candidate b-glucuronidase [[Eubacterium] eligens ATCC 27750]
6BJW_A	1.92e-252	1	596	25	633	ChainA, Glycoside Hydrolase Family 2 candidate b-glucuronidase [[Eubacterium] eligens ATCC 27750]
6D4O_A	2.61e-252	1	596	4	612	Eubacteriumeligens beta-glucuronidase bound to an amoxapine-glucuronide conjugate [Lachnospira eligens]
6U7I_A	1.93e-245	1	596	1	597	Faecalibacteriumprausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii],6U7I_B Faecalibacterium prausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii],6U7I_C Faecalibacterium prausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii],6U7I_D Faecalibacterium prausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P05804	3.14e-174	1	587	1	588	Beta-glucuronidase OS=Escherichia coli (strain K12) OX=83333 GN=uidA PE=1 SV=2
O97524	2.74e-148	1	590	27	628	Beta-glucuronidase OS=Felis catus OX=9685 GN=GUSB PE=1 SV=1
O18835	1.09e-147	1	590	27	628	Beta-glucuronidase OS=Canis lupus familiaris OX=9615 GN=GUSB PE=1 SV=1
P06760	1.58e-146	1	590	27	625	Beta-glucuronidase OS=Rattus norvegicus OX=10116 GN=Gusb PE=1 SV=1
P12265	4.45e-146	1	590	27	625	Beta-glucuronidase OS=Mus musculus OX=10090 GN=Gusb PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000034	0.000003	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003245_00452.