CAZyme Information: MGYG000003230_00995

Basic Information

• Species: CAG-313 sp900539265
• Lineage: Bacteria; Firmicutes; Bacilli; ML615J-28; CAG-313; CAG-313; CAG-313 sp900539265
• CAZyme ID: MGYG000003230_00995
• CAZy Family: GH3
• CAZyme Description: Beta-hexosaminidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
519		58076.16	5.3041

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003230	1876172	MAG	United States	North America

• Gene Location: Start: 102; End: 1661 Strand: +

Full Sequence      

MKDIKNMTLKEKVGQMIGLAFSGDKYSSELKMQVEEIEAGLIIYFKDNCITPRQIFELNK
EINKNAKIPPFIAIDQEGGMVARVTEGIVQSPGAMAISATNNKENAYILAKNMGIELRNL
GFNFNFAPVGDVNNNPKNPVINVRSYSEDPNVVAEYMEEAVKGYHEAGLMTSVKHFPGHG
DTAVDSHVGLPIVDFDKERLDKMELVPFKKVVDLNLPGIMASHVLYTRYDDKFPTTLSKK
VITGLLREEMGFKGLVVTDSLTMSAVFDNFSLEEIVYNGFNSGCDILLLCGARNVQMQKE
FAEIALRLAEEGKIPLSIIDAAVERILKYKRQYNVGQMAECFDDIKADLEQPTRMKFAEK
VAEDSITLLKNNNNLLPLTKEDKILVVFPQIKVVTLVENDDNTLSCLADYMPFSVDKHYI
SIAPTKIEQTNLLKKIHQYDKIIYCSYNANFNSSQADLINKIEKNKLIVVAVRTPYDINV
LDVPTYVCAYEASVLSFKALAKYLTGEIEAKGILPVSVK

Enzyme Prediction     

EC	3.2.1.52

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	64	288	8e-60	0.9722222222222222

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1472	BglX	2.62e-90	7	352	1	332	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
pfam00933	Glyco_hydro_3	3.08e-89	8	327	1	314	Glycosyl hydrolase family 3 N terminal domain.
PRK05337	PRK05337	5.21e-53	35	298	16	288	beta-hexosaminidase; Provisional
PRK15098	PRK15098	2.67e-13	4	389	42	418	beta-glucosidase BglX.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
VEU80306.1	1.79e-118	3	516	5	521
ART78538.1	4.53e-118	7	518	1	522
AST92910.1	2.79e-117	1	518	1	524
AUD66191.1	7.71e-113	3	516	7	524
VEU82803.1	9.65e-113	3	516	5	520

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6K5J_A	2.34e-105	5	515	9	528	Structureof a glycoside hydrolase family 3 beta-N-acetylglucosaminidase from Paenibacillus sp. str. FPU-7 [Paenibacillaceae]
3BMX_A	2.50e-78	4	447	39	515	Beta-N-hexosaminidase(YbbD) from Bacillus subtilis [Bacillus subtilis],3BMX_B Beta-N-hexosaminidase (YbbD) from Bacillus subtilis [Bacillus subtilis],3NVD_A Structure of YBBD in complex with pugnac [Bacillus subtilis],3NVD_B Structure of YBBD in complex with pugnac [Bacillus subtilis]
3LK6_A	7.59e-78	4	447	13	489	ChainA, Lipoprotein ybbD [Bacillus subtilis],3LK6_B Chain B, Lipoprotein ybbD [Bacillus subtilis],3LK6_C Chain C, Lipoprotein ybbD [Bacillus subtilis],3LK6_D Chain D, Lipoprotein ybbD [Bacillus subtilis]
4GYJ_A	1.52e-77	4	447	43	519	Crystalstructure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1) [Bacillus subtilis subsp. subtilis str. 168],4GYJ_B Crystal structure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1) [Bacillus subtilis subsp. subtilis str. 168],4GYK_A Crystal structure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1211) [Bacillus subtilis subsp. subtilis str. 168],4GYK_B Crystal structure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1211) [Bacillus subtilis subsp. subtilis str. 168]
4ZM6_A	6.00e-72	9	518	9	527	Aunique GCN5-related glucosamine N-acetyltransferase region exist in the fungal multi-domain GH3 beta-N-acetylglucosaminidase [Rhizomucor miehei CAU432],4ZM6_B A unique GCN5-related glucosamine N-acetyltransferase region exist in the fungal multi-domain GH3 beta-N-acetylglucosaminidase [Rhizomucor miehei CAU432]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P40406	1.37e-77	4	447	39	515	Beta-hexosaminidase OS=Bacillus subtilis (strain 168) OX=224308 GN=nagZ PE=1 SV=1
P48823	8.97e-63	29	518	54	596	Beta-hexosaminidase A OS=Pseudoalteromonas piscicida OX=43662 GN=cht60 PE=1 SV=1
Q0A911	2.51e-38	16	290	7	283	Beta-hexosaminidase OS=Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1) OX=187272 GN=nagZ PE=3 SV=1
Q6LJ30	9.32e-38	23	298	10	282	Beta-hexosaminidase OS=Photobacterium profundum (strain SS9) OX=298386 GN=nagZ PE=3 SV=1
Q0AF74	1.75e-37	19	290	8	283	Beta-hexosaminidase OS=Nitrosomonas eutropha (strain DSM 101675 / C91 / Nm57) OX=335283 GN=nagZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000053	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003230_00995.