CAZyme Information: MGYG000003193_00052

Basic Information

• Species: CAG-831 sp900544175
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; UBA932; CAG-831; CAG-831 sp900544175
• CAZyme ID: MGYG000003193_00052
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1145	MGYG000003193_1|CGC1	128423.2	4.7537

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003193	2486830	MAG	United States	North America

• Gene Location: Start: 56545; End: 59982 Strand: +

Full Sequence      

MKNILIPLALVLCPAVLSAQGQKDRLPYWQDVQTVEVNREYPRTAFMSYPDSATAAAYRY
ENSPRYLLLNGVWKFYFVDAYRDLPENIADPSVDASGWNDINVPGNWEVQGFGTAIYTNH
GYEFKPRNPTPPVLPEDNPVGVYRREFTVPEAWDGSEIFLNVDGAKSGVYVYLNGREVGY
SEDSKTSAEFAVSDFLVPGVNTLVLKIYRWSTGSYLECQDFWRMSGIERDVYLWCQPREA
ALKDFTVVSTLDDTYTEGLFSLRAVLKNTSASGSEAVLRGTLTGPDGAVVWSDVRNVPLD
AGSTSEVLFENRIPDVQPWSAEHPDLYRLTMTVYADGKAVETVPYHVGFRKFEIKPSGEL
SPEGRPYVLFYVNGQPVKLKGVNTHEHNPMTGHYVTEELIRRDLELMKQNNINTVRLAHY
PQGRRFYELCDEYGLYVYDEANIESHGMYYNLRKGGTLGNNPEWLAAHMDRTVNMYERNK
NYPCITVWSLGNEAGNGYNFYQTYLWIKDRERGGMDRPVCYERAQWEWNSDMYVPQYPGA
DWLRQIGRSGSDRPVVPSEYSHAMGNSSGSLSLQWDEIYRYPNLQGGYIWDWVDQGIWQD
RDGGFWAYGGDFGVNAPSDGNFLCNGIVNPDRNPHPAMTEVKHAYQNAAFRPSGYAEDPT
AERTLATGRLAFSSLPSGGKVSVDVINRFYFTDLDAYDFTYELTADGRVAASGTLAVSAA
PQDTVTVEIPVPALKSSGTEYFLNLYMKQRSEVLGIPAGHVLASDQIAFPVTGERPVYAE
GRGPALSVDSVSSEGVISIFSPSVEWVFDRSAAAVTSYKVRGVEYFAGGFGLRPNFWRGP
NDNDYGNGAPKRLQIWKTSSQELRVGAVSVAMDGADAVMTVEYLLAAGNKYIAEYRVHPD
GVVDARLHFTPALMPEVESEASEATLTATYTPGQESRVGLKARPEVPRIGVRFRLPVAMH
GVTWYGRGPGENYIDRSRGSLVGLYSSSAEAMYFPYVRPQENGHRSDTRWAAFYGGGNGL
MVVADSTIGFSALRNPVEDFDSEEAVEHDYQWPNFTPEQIASKDPADAKDVLRRMHHIND
IVPQDYVEVCIDMRQQGVAGYDSWGDRPEPQYQLPADRDYTWSFTLVPVKNARDAQRKAL
YDYGL

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	57	982	2.8e-201	0.973404255319149

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK09525	lacZ	0.0	29	1126	17	1024	beta-galactosidase.
PRK10340	ebgA	0.0	29	1145	4	1017	cryptic beta-D-galactosidase subunit alpha; Reviewed
COG3250	LacZ	4.78e-131	69	976	15	800	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
smart01038	Bgal_small_N	9.11e-90	801	1126	2	272	Beta galactosidase small chain. This domain comprises the small chain of dimeric beta-galactosidases EC:3.2.1.23. This domain is also found in single chain beta-galactosidase.
pfam02836	Glyco_hydro_2_C	2.33e-87	369	650	7	302	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUR46524.1	0.0	21	1143	2	1101
QUT33020.1	0.0	18	1143	10	1112
QRN02091.1	0.0	21	1143	2	1101
QDH57581.1	0.0	21	1143	2	1101
QNL36573.1	0.0	17	1143	23	1126

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6SD0_A	4.26e-212	26	1126	1	980	Structureof beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_B Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_C Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_D Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8]
6S6Z_A	8.19e-212	27	1126	1	979	Structureof beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_B Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_C Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_D Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_E Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_F Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_G Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_H Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8]
3DEC_A	3.25e-203	27	1130	5	998	ChainA, Beta-galactosidase [Bacteroides thetaiotaomicron VPI-5482]
3BGA_A	1.22e-198	23	1130	4	1002	Crystalstructure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482 [Bacteroides thetaiotaomicron VPI-5482],3BGA_B Crystal structure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482 [Bacteroides thetaiotaomicron VPI-5482]
3MUY_1	1.79e-142	29	1009	16	942	Chain1, Beta-D-galactosidase [Escherichia coli K-12],3MUY_2 Chain 2, Beta-D-galactosidase [Escherichia coli K-12],3MUY_3 Chain 3, Beta-D-galactosidase [Escherichia coli K-12],3MUY_4 Chain 4, Beta-D-galactosidase [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q56307	2.33e-211	26	1126	1	980	Beta-galactosidase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=lacZ PE=1 SV=2
O52847	1.39e-188	27	1129	18	1034	Beta-galactosidase OS=Priestia megaterium (strain DSM 319 / IMG 1521) OX=592022 GN=bgaM PE=3 SV=1
Q6LL68	7.44e-167	29	1131	12	1030	Beta-galactosidase OS=Photobacterium profundum (strain SS9) OX=298386 GN=lacZ PE=3 SV=1
A5F5U6	1.40e-164	29	1126	13	1016	Beta-galactosidase OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) OX=345073 GN=lacZ PE=3 SV=2
B4S2K9	1.20e-156	29	1125	13	1036	Beta-galactosidase OS=Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype) OX=1774373 GN=lacZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001248	0.986747	0.011118	0.000331	0.000275	0.000259

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003193_00052.