CAZyme Information: MGYG000003192_01041

Basic Information

• Species: UBA5416 sp900539175
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; UBA5416; UBA5416 sp900539175
• CAZyme ID: MGYG000003192_01041
• CAZy Family: GH16
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2065	MGYG000003192_15|CGC1	228260.08	4.2316

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003192	4033193	MAG	United States	North America

• Gene Location: Start: 53097; End: 59294 Strand: -

Full Sequence      

MKQKAKRLLSVVLAFLMVIGIMPSGGLMTVPVMAAAEKFEINMSQYNESEMEISDGYSNG
EPFGCMWRQGNVKFTGNGTELSITEDSVSGSSYQYAGAEYRTRERYGYGYYEVTMKPIKH
SGVVSSFFTYTGPSDGTPWDEIDIEFLGKDTTKVQINYYVNGTGNHERMLDLGFDASEAF
HTYGFDWQSDHITWYVDGKDVYTAYGDANGLPVNPGKIMMNVWPGTGVDGWLGAFDGKTP
LTAEYKAASYSQTPPGTEGPTVTPTPTAAPTVTPTVTPTPTGGNPSFPSNVTGKVTFFDL
YTDKEINPNTSYGFEDWEYYDWRDMYSSWEDWNDESKKTNPNPASKPAGTDRSWTSIGRA
FSQNVNPKIFEYWNKWYSENWYEEKPRVNPLYFGDFRPFFRIDNDENRTDEEKSLGIFWT
DPWYANNNVYRTPYNSSYTYDRKSYMEGIHGGEGWNFSWFLNRGVDNPWSDEESAKNAGA
AVQGIVSDCLDSDGNITERTSGVPLPQFNKAFYDGNSTIGKMYEPMEFPFVVRERNGANY
YEFDSGVGNSTYHDTVRMNADGTALTYSNKEEDVVYGIQGGGARTPGFFPFNDPQDSLRD
DGSEEHKYDGSGNKLNYGFGMKLEVDFTMDSDGMIKTKSGERIPATFEFSGDDDVWIFVD
GKLALDMGGDHGVATGRLNFADRTAVVEQVKGIKDYRDGSGNIVEDANEAVKAEQVFNHI
GEGGKDERDAACYNVGVNQKTAKFDIDTTNPKAIHTLTIFYEERGMYESNFRASFNLNLP
TKLMTTNQVNVDKVNQGLRQAAKEVAQNDAFTFKVADKDGKSVGEKDYKIQNGEVKLGED
GKMTIKDGQSATFDAQFDRGSELSLVQSGNDRYDTSWTMTGSSKDNSEVQIASSAQESRD
SLTVGDSRVEGHENGAFKLENTDTDESVPVTVRVDYKQEPKTNSFIIQKVLDEAAASDEI
FGFSVTFSDIFGSISAEEAYDIVYDIYTAEGELVQENALASGGIVYLKAGQYALIKDIPV
GTKYKVVENEKVGYTVKNLVSEQGNGDIDIMTDQTTGTITAAVANDGKSADKFIYTNGIK
KIDDSFLVEVGKSNTLSVIPKEETDGELTGDLKEISDSWNASEASKKGLVFVVNGQPQLA
TDGKPFTSIVADGVTYEVEYDSQGTPVLRVTPKDQEVQGKDVITVQYQLVELNDDGTVKY
NDNELAKITPVIETTSYFYKANGDIYVLDYGLDVNLANKTEDRSGIFENDQLVNQSLAGT
TSTYWSTKAGKNAADSSADKVLESGLIKNGSFDEGDASYEWFIDQNAAANSTVENQEAKY
TIKNTGDQEWQVQLKQENINLERNQWYKLTLDAKSDLDRKLKFMIQRNGNQPGKDWTTYT
VETVQLTSDYQTFEVKFQMQEPTDSESVFCIAMGAVDGEQISTEHQIFIDNISLEKISPS
SNIEAANSAEPADYHAVAGEYGKIIPAEGPEAYTITDQANPKVTYTMSKFLEGGDEYAYG
VMVKKADISEKQTSNRFRLQVTSDVTMMPANVVYYEDNFNAGSTDDNSSPAIIYTGTYET
EGTSLDLVQSNGQTEQYGHDGAYNTMDNQKDSGGSSTKLTADGYNTKAIFTFTGTGFDIL
ARTTRKTAGIVYTIEKYDQAADKYSFFRMGAVDTYYINGDLYQLPVIHEEGMAHGTYRVT
LGIKQTGSYLITKDENGEVIKEDDTRKYVVYLDGIRIYNPLGESGDQRYIADEQGVTVSE
IPELIVGNGTVTEKGIIDETGIGIINSQVVEGATAVIASYSSDDCSLEALGNTQTEVATG
DSSAGTESLVTYLNAGPNNELYLDEAAALAFVVKATDNTNTLQIEAKLVDIQNSEGSKEG
NGLDLRVLSNKNGVVEEKTIDTIKSSTAMYYKIPVEDCISLDNGYYLIVILGNSDVMGSA
NDTHVLSFSNLKYKNYTIGSPYNTSVYDTSAYLEADEEEPNMFVSVNLAAGLKKNTWQSF
DNHSVTLTNNVFGDAEPEFTMYYVNAKGDKVKLTVAARRVKDANSEYQLRFKTPNAKGNF
PVEIHYVVDGEESADYIATTMKVVK

Enzyme Prediction     

No EC number prediction in MGYG000003192_01041.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	44	246	2.9e-84	0.9660194174757282
CBM4	1286	1414	2e-31	0.9841269841269841

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd02175	GH16_lichenase	6.49e-106	46	251	8	211	lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure.
COG2273	BglS	5.31e-51	34	252	42	266	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
pfam00722	Glyco_hydro_16	1.24e-47	67	239	1	163	Glycosyl hydrolases family 16.
cd00413	Glyco_hydrolase_16	2.24e-38	57	244	17	201	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
cd02183	GH16_fungal_CRH1_transglycosylase	1.45e-26	69	225	16	166	glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACZ98606.1	1.04e-81	39	246	51	254
BCZ45550.1	1.96e-81	17	250	13	243
AFK65349.1	2.41e-81	9	251	13	241
AFH62447.2	2.41e-81	9	251	13	241
AFC30180.1	4.48e-81	9	251	13	241

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3I4I_A	2.66e-81	46	250	31	231	Crystalstructure of a prokaryotic beta-1,3-1,4-glucanase (lichenase) derived from a mouse hindgut metagenome [uncultured murine large bowel bacterium BAC 14],3I4I_B Crystal structure of a prokaryotic beta-1,3-1,4-glucanase (lichenase) derived from a mouse hindgut metagenome [uncultured murine large bowel bacterium BAC 14]
1MAC_A	3.00e-76	43	251	8	210	CrystalStructure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans],1MAC_B Crystal Structure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans]
1BYH_A	8.11e-76	44	251	11	212	MOLECULARAND ACTIVE-SITE STRUCTURE OF A BACILLUS (1-3,1-4)-BETA-GLUCANASE [synthetic construct],1GLH_A Cation Binding To A Bacillus (1,3-1,4)-Beta-Glucanase. Geometry, Affinity And Effect On Protein Stability [Paenibacillus macerans],2AYH_A Crystal And Molecular Structure At 1.6 Angstroms Resolution Of The Hybrid Bacillus Endo-1,3-1,4-Beta-D-Glucan 4- Glucanohydrolase H(A16-M) [hybrid]
3WVJ_A	3.42e-75	44	250	13	213	ChainA, Beta-glucanase [Acetivibrio thermocellus ATCC 27405],3WVJ_B Chain B, Beta-glucanase [Acetivibrio thermocellus ATCC 27405]
3O5S_A	4.59e-75	20	252	13	237	CrystalStructure of the endo-beta-1,3-1,4 glucanase from Bacillus subtilis (strain 168) [Bacillus subtilis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P45797	4.51e-76	43	251	34	236	Beta-glucanase OS=Paenibacillus polymyxa OX=1406 GN=gluB PE=3 SV=1
P23904	2.79e-75	43	251	33	235	Beta-glucanase OS=Paenibacillus macerans OX=44252 PE=1 SV=2
P04957	3.91e-74	46	252	41	241	Beta-glucanase OS=Bacillus subtilis (strain 168) OX=224308 GN=bglS PE=1 SV=2
A3DBX3	2.21e-73	44	263	42	255	Beta-glucanase OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=licB PE=1 SV=1
P07980	1.97e-72	44	250	36	236	Beta-glucanase OS=Bacillus amyloliquefaciens OX=1390 GN=bglA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000246	0.999152	0.000154	0.000165	0.000136	0.000130

TMHMM  Annotations     

start	end
12	34