CAZyme Information: MGYG000003179_00546

Basic Information

• Species: UMGS1004 sp900547645
• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; Borkfalkiaceae; UMGS1004; UMGS1004 sp900547645
• CAZyme ID: MGYG000003179_00546
• CAZy Family: GH115
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1471		165457.3	4.1044

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003179	1597931	MAG	United States	North America

• Gene Location: Start: 1526; End: 5941 Strand: -

Full Sequence      

MKKKRISFGVILAMIVMALSMVFAACAPSGGDDNGSSDADAFNLISGNRAAVICVEGTNA
IGSTDGDYPGVVRAAYDLQSDVELITGVKPVVTKELKGYTDYAVIIGTVGKSSAIAEIAE
NGKIDTSSIEGKWECYLVQTVQDPFNDGTVGTALVIAGSDKRGSIYGIYSVSEMLGMTPW
NFFADSVPAQKDNLVLAGDYRYASREPSVKYRGIFINDEECLVDWARSLDEGKNLGPNFY
KKLFECLLRMKSNYLWPGMHACSDAFSDYMENPVNADYYGIVIGTSHCDMLLRNNLNEWD
TFLSEYKAEHPEYAGVDIEYDYTLFPEVLQDYWRGSVRENKDYEVQWTLGIRGPHDDPLE
CADIDKAPWYGDKNLLMETVIEDQRQILREELDNPTLEDVFMMFIPYKEVQAIYNNGLEV
PEDVTIMWVDDNHGFIRDVPNEVERQRSGGHGIYYHNSYWGPDNESYMWVNSMPLTLMYE
ELNKAYDNGIQTAWVLNPGDTVPYMFEIGFVMDLGYDIEKYNNENVFETYVSQVAAREFG
AEFSEDLTQIVREYTQMTNARKLEQMSVDIFTDAYGDEMERRAAQYKDLWERAEAMYDAI
PARQRDCFYELFLFEIRCAYFTNAEFYYAQKGNVAYAQGRNATAWNCFMKSKEFNEKRKD
EISYYNVVLQDGKWNHLVDPEVYHSPAMAGFASGSPTFTLGETEMGVIAEGEQAETQNSV
LSFGNYAQGRKYVDVFNKGAGSFEFQVSDDSDFILVSETEDVVTDEVRLWISIDWDKVSA
SDSGTVSISANGQTKTITVNVQKDDYQLGEKTYVEQDGYVSIEAEHYSASRETDTTYWSV
IKDLGRVSGDMVTAYTETLVGYGESNFESQAPYLEYNVYFANTGSFETEIYRLPSLRSRD
RIRFAVSVNNETPIVVEGENDYGTNNPAWEEGVFTQIIKHQVTLDIKNAGLNTIRIYMID
PYITIDKLVVYTGEKLDSYFGPLESYNTTYNTAPADKAYYESYYESAYELPEGYDIGRGY
GTGAFIEQNGKLAIEAEAAEIGTEETGAWKEGRWLVAQTDAGISMRTEDLRVDYDGRWET
EAPTLNYTIVVNTPGTYRVWVNINNPSPRSSCYAVGLDGRYQFVIADFNWSREEVFQWRQ
GSTVNLTAGVHTFTVYCSQDGVAIDKIYLSRTSETPNNNTFVQTARTPIDGDAQAQIRDA
ALRASLSSAVNAIGNYYNVVTGDGLGEYGEAEYAALMGAIDNAYALIDSADALEDAQVTQ
ALDDLESALNALYASRNMTDGDREYLLYECFDKAYTGLEPFGFTYHTKYLTPDVSVAEGE
DGKYFNIRTFKEQANQETAFVQYAFEAQSGIFTMETRASFNEGEWGSLYLLNENGDNAIC
IAFEFADNAYNLVAYNGNVKTVIGHYERNVAVDIKVVADVAENTFDVYVGGELVAEGLAF
RMSTQSVGTVMFGSSSRNADFRLYEMKIYGE

Enzyme Prediction     

No EC number prediction in MGYG000003179_00546.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH115	43	686	1.9e-183	0.8249641319942611

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam15979	Glyco_hydro_115	1.50e-131	206	564	2	333	Glycosyl hydrolase family 115. Glyco_hydro_115 is a family of glycoside hydrolases likely to have the activity of xylan a-1,2-glucuronidase, EC:3.2.1.131, or a-(4-O-methyl)-glucuronidase EC:3.2.1.-.
pfam17829	GH115_C	3.20e-50	812	984	1	172	Gylcosyl hydrolase family 115 C-terminal domain. This domain is found at the C-terminus of glycosyl hydrolase family 115 proteins. This domain has a beta-sandwich fold.
pfam17829	GH115_C	2.03e-11	1025	1167	2	156	Gylcosyl hydrolase family 115 C-terminal domain. This domain is found at the C-terminus of glycosyl hydrolase family 115 proteins. This domain has a beta-sandwich fold.
pfam03648	Glyco_hydro_67N	0.003	66	169	28	118	Glycosyl hydrolase family 67 N-terminus. Alpha-glucuronidases, components of an ensemble of enzymes central to the recycling of photosynthetic biomass, remove the alpha-1,2 linked 4-O-methyl glucuronic acid from xylans. This family represents the N-terminal region of alpha-glucuronidase. The N-terminal domain forms a two-layer sandwich, each layer being formed by a beta sheet of five strands. A further two helices form part of the interface with the central, catalytic, module (pfam07488).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASA21655.1	4.02e-260	11	1441	9	1468
QGH34463.1	1.32e-210	64	1186	22	1223
AFO77172.1	1.11e-200	36	994	44	982
ADJ45460.1	1.11e-200	36	994	44	982
AGT84300.1	1.11e-200	36	994	44	982

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4ZMH_A	7.25e-131	67	985	31	933	Crystalstructure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40],4ZMH_B Crystal structure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40]
6NPS_A	9.51e-106	70	986	31	964	Crystalstructure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112],6NPS_B Crystal structure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112]
5BY3_A	1.85e-87	42	679	20	616	Anovel family GH115 4-O-Methyl-alpha-glucuronidase, BtGH115A, with specificity for decorated arabinogalactans [Bacteroides thetaiotaomicron VPI-5482]
7PUG_A	2.07e-86	70	973	37	822	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]
4C90_A	6.74e-86	70	985	64	849	Evidencethat GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C90_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_A Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000000	0.000007	1.000014	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
7	29