CAZyme Information: MGYG000003072_00439

Basic Information

• Species: Paenibacillus amylolyticus
• Lineage: Bacteria; Firmicutes; Bacilli; Paenibacillales; Paenibacillaceae; Paenibacillus; Paenibacillus amylolyticus
• CAZyme ID: MGYG000003072_00439
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
858	MGYG000003072_1|CGC8	98185.19	5.0436

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003072	7042795	MAG	China	Asia

• Gene Location: Start: 447333; End: 449909 Strand: -

Full Sequence      

MKTYSSSQDLSGQWKIQHFEVGEKRAMDVAAATLDDRFWIGAEVPGDVHAALVERSIIDP
PYYGHNDAKSRWIEQKEWWYRTTFNLVRDAETEEHFELVFEGLDTFATVYVNGHEVGKTA
NMLMSHAFDVTSLVRSGWNAIAVKFDPLHMHHRDKETFDWSSYTKERPWLRKAAMNFGWD
WGPRMVTVGIWGAVRLEKRTIAKLESVYARTESVSSELAVLHVTADVKSVLSYRNRQKRE
QAVVATCNVRLLDRSGHEVAGVTELPVEGGIADTTLNVTSPQLWWTHDLGEPYLYTLEVT
LFADGVEVDRYSEPYGLRTIELALHNERGEDAFTFILNGVKVYAKGANWIPADHLIGAIP
NSRYRELVELSVEGHMNMLRVWAGGIYEKDVFYDECDRQGVLVWQDFAFANALFPDFNRD
FMDNVRQEVENNVLRLRNRASLALWCGNNEIDWLYDMKSASGDITSPFYGEQIYHELIPE
VLERLDLSRPYWPSSPFGDSNGQDANDPDVGDRHNWQVWHGSVYPRKHGEPPLLDYSIEG
VTFKNYKNDHALFSSEFGMHASANRYTLEKNMPAGQFYWGSPEMAYRNKDTNHQKGILLM
EGYTGIPQDIEEYMNYSMLTQAEGLRYGIEHFRRINHRNSGALVWQLNDSWPGTSWSMID
YELLPKASFYYGKIFFHPVLLSLEHEPGEPLALWVVNDTRDVLKGELRLNAYALNGEKIY
SSSYAVEVTSQSSRCIVELTEVEVLQGRRAEEVMVELVSEGFAAPSNRYFLRDPKDVTLP
ESQLSVHVNEEEQSVTVTASGGIARLVKLELPLGRVRFSDNYFDLLPGESRTVRLRHPEQ
TSLPLTELRVSAMNGREG

Enzyme Prediction     

No EC number prediction in MGYG000003072_00439.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	3	756	1.5e-108	0.7180851063829787

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	5.29e-81	3	836	8	785	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam17753	Ig_mannosidase	1.89e-15	783	854	3	77	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
PRK10150	PRK10150	2.24e-15	79	346	68	296	beta-D-glucuronidase; Provisional
pfam00703	Glyco_hydro_2	2.50e-15	202	318	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
pfam17786	Mannosidase_ig	3.72e-15	689	776	1	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
APO45075.1	0.0	1	858	1	858
QZN73430.1	0.0	1	858	1	858
QLG41524.1	0.0	1	857	1	857
QOS78215.1	0.0	1	857	1	857
QKS59560.1	0.0	6	858	8	862

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5N6U_A	1.04e-147	36	854	40	829	Crystalstructure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_B Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_C Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_D Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12]
2VJX_A	6.09e-125	39	838	25	819	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	6.40e-125	39	838	27	821	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	6.56e-125	39	838	27	821	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	1.72e-124	39	838	25	819	Structureof the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q4WAH4	6.43e-117	15	845	3	838	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
A1D911	6.43e-117	15	845	3	838	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
B0YBU9	2.46e-116	15	845	3	838	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1
Q0CCA0	9.32e-115	42	845	30	836	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2
A1CGA8	1.37e-114	44	845	32	838	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000076	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003072_00439.