CAZyme Information: MGYG000003059_00160

Basic Information

• Species: CAG-180 sp900545625
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; CAG-180; CAG-180 sp900545625
• CAZyme ID: MGYG000003059_00160
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
852	MGYG000003059_2|CGC1	97990.42	6.4614

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003059	1925341	MAG	United States	North America

• Gene Location: Start: 12278; End: 14836 Strand: +

Full Sequence      

MMQISLNGEWKFREAQKGEWLPAKVPGCNYLDLLRAEKIPDPFVGLNENEVYWVAETDWE
YERTFTVPAELLDCDRVELQCATLDTICDVFVNDTLVGKGENAHRRYAFDVKSALKAGEN
RIRILFYSPVNYVKAKQEVEKCPRNSNGMDGIPHIRKPQCHFGWDWGPVLPPSGISGDIG
IFGYSGARITDLLIEQRHEDGKVRLSVCGEVERLSAGTPAYVLRILTPAGELLSETCGEL
DDAGRVHLTIEIENPELWWTRDLSEKVQQPLYTVSFELLCDGAQTDKATKKIGLRTFELN
REKDEYGENFQFRINGVPLFIKGANWIPPDSFATRYTDARMEYDMAAMRFSNFNMLRIWG
GGYYERDALYDKCDRDGILIWQDFCFACQGYPFFDEALLENIHGEVVDNVRRLRHHACLA
LWNGNNEIETMSIAWQARRKYIDWAEKFFYHILPEWLEKLDAQTPYIPGSPCGTGYLQGV
DRDGVGDTHLWAVWHGLQPLTYYRRRMTRFCSEFGFESLPDQKTIARYAAPADYDLNSAV
FLAHQKCAGGNMKMAFYIASRFRLPKHFMDYVYLSQICQEECVRDATEHWRRNKGRCNGS
MYWQFNDCWPVCSWAGLDYYGNYKALQYAARHFNAPFTVSVEDTKERLRVFALNDTRQDR
RATLSWRLLGFDGEQFASEKKSITVNALQNEIVFDLPVSRLSAKGNLKRAVFVADLMDGE
TLLARKTVLFDKEKNLALPKTQVDMHVRVSDGAAQIELTAKRYTRFLQVHSKSNLLPFSD
NFFDLLPGETKRIVQPVRDGVTAEQVKNDLELFSAGDVVPRASRLSDALTRARVFLKPLS
IGNFFYYQRIPK

Enzyme Prediction     

No EC number prediction in MGYG000003059_00160.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	3	751	1e-108	0.7606382978723404

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	3.34e-101	1	796	10	785	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam17786	Mannosidase_ig	6.01e-15	646	735	1	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
pfam17753	Ig_mannosidase	1.14e-10	740	817	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
pfam00703	Glyco_hydro_2	7.01e-09	187	295	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK09525	lacZ	1.84e-08	5	427	54	462	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCJ98156.1	1.33e-223	1	831	1	837
CUS03525.2	2.54e-218	1	817	1	817
BAJ62595.1	1.08e-212	10	814	14	824
AUG59052.1	3.65e-211	2	819	1	818
BAL98552.1	9.13e-204	25	817	1	832

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VJX_A	2.48e-158	6	812	11	827	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	2.63e-158	6	812	13	829	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	2.70e-158	6	812	13	829	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	7.12e-158	6	812	11	827	Structureof the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]
6BYE_A	2.27e-156	1	789	3	826	Crystalstructure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306],6BYE_B Crystal structure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A1D911	2.67e-110	1	793	4	826	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
I2C092	4.67e-110	4	793	7	836	Beta-mannosidase B OS=Thermothelomyces thermophilus OX=78579 GN=man9 PE=1 SV=1
A1CGA8	1.97e-109	1	793	4	826	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1
Q4WAH4	3.84e-109	1	793	4	826	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
B0YBU9	1.46e-108	1	793	4	826	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000069	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003059_00160.