CAZyme Information: MGYG000003008_00440

Basic Information

• Species: TM7x sp900555675
• Lineage: Bacteria; Patescibacteria; Saccharimonadia; Saccharimonadales; Saccharimonadaceae; TM7x; TM7x sp900555675
• CAZyme ID: MGYG000003008_00440
• CAZy Family: GH57
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
400		47142.06	5.7137

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003008	664737	MAG	United States	North America

• Gene Location: Start: 3017; End: 4219 Strand: +

Full Sequence      

MNKNKGIVLYLHVHQPWRIREYSIFDVAWKHNYFSGGQNQDQDNQKIFQKVAEKSYLPMN
ALLEKLLKKYPDFKISLSFSGTFLEQAERFNPEVLESFKRLVKTGQVEILSSPYHHSLAF
FYSRKEFECQVELHRWKIREIFGAETRVLANTELAYSDDLAKWAEQDGFKGVLAEGWDPI
LNWRSPNYVYRPRGTKKIGLLLKNYRLSDDLAFRFSDRKWNEWPLTADKFNTWVEDSVRY
APLLNLFMDYETFGEHQWAESGIFGFFEKFVDKWLSAGGNTFYTVSEALDANAPAGEISM
PSPVTWADAERDLTAWNGNSLQKEALRYVYELEDEVLDSKDEGLISDWRKLQTSDYFYYM
GTKNFTDGDVHAYFSPYDSPYDAFLYYMNTIRDMRSRLRK

Enzyme Prediction     

No EC number prediction in MGYG000003008_00440.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH57	8	327	1.8e-60	0.8015665796344648

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd10795	GH57N_MJA1_like	7.00e-160	6	319	1	306	N-terminal catalytic domain of a thermoactive alpha-amylase from Methanococcus jannaschii and similar proteins; glycoside hydrolase family 57 (GH57). The subfamily is represented by a thermostable alpha-amylase (MJA1, EC 3.2.1.1) encoded from the hyperthermophilic archaeon Methanococcus jannaschii locus, M J1611. MJA1 has a broad pH optimum 5.0-8.0. It exhibits extremely thermophilic alpha-amylase activity that catalyzes the hydrolysis of large sugar polymers with alpha-l,6 and alpha-l,4 linkages, and yields products including glucose polymers of 1-7 units. MJ1611 also encodes another alpha-amylase with catalytic features distinct from MJA1, which belongs to glycoside hydrolase family 13 (GH-13), and is not included here. This subfamily also includes many uncharacterized proteins found in bacteria and archaea.
pfam03065	Glyco_hydro_57	1.00e-69	8	301	1	293	Glycosyl hydrolase family 57. This family includes alpha-amylase (EC:3.2.1.1), 4--glucanotransferase (EC:2.4.1.-) and amylopullulanase enzymes.
COG1449	COG1449	1.75e-64	2	388	83	492	Alpha-amylase/alpha-mannosidase, GH57 family [Carbohydrate transport and metabolism].
cd01022	GH57N_like	1.30e-41	11	318	4	313	N-terminal catalytic domain of heat stable retaining glycoside hydrolase family 57. Glycoside hydrolase family 57(GH57) is a chiefly prokaryotic family with the majority of thermostable enzymes coming from extremophiles (many of these are archaeal hyperthermophiles), which exhibit the enzyme specificities of alpha-amylase (EC 3.2.1.1), 4-alpha-glucanotransferase (EC 2.4.1.25), amylopullulanase (EC 3.2.1.1/41), and alpha-galactosidase (EC 3.2.1.22). This family also includes many hypothetical proteins with uncharacterized activity and specificity. GH57s cleave alpha-glycosidic bonds by employing a retaining mechanism, which involves a glycosyl-enzyme intermediate, allowing transglycosylation.
cd10796	GH57N_APU	1.60e-16	7	318	1	313	N-terminal catalytic domain of thermoactive amylopullulanases; glycoside hydrolase family 57 (GH57). Pullulanases (EC 3.2.1.41) are capable of hydrolyzing the alpha-1,6 glucosidic bonds of pullulan, producing maltotriose. Amylopullulanases (APU, E.C 3.2.1.1/41) are type II pullulanases which can also degrade both the alpha-1,6 and alpha-1,4 glucosidic bonds of starch, producing oligosaccharides. This subfamily includes GH57 archaeal thermoactive APUs, which show both pullulanolytic and amylolytic activities. They have an acid pH optimum and the presence of Ca2+ might increase their activity, thermostability, and substrate affinity. Besides GH57 thermoactive APUs, all mesophilic and some thermoactive APUs belong to glycoside hydrolase family 13 with catalytic features distinct from GH57. This subfamily also includes many uncharacterized proteins found in bacteria and archaea.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QCT41485.1	2.14e-300	1	400	1	400
AJA06438.1	2.14e-300	1	400	1	400
QCT40666.1	5.78e-219	1	398	1	397
QJU10944.1	8.21e-219	1	398	1	397
QHU92976.1	2.35e-218	1	398	1	397

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1K1X_A	1.43e-10	47	349	22	305	Crystalstructure of 4-alpha-glucanotransferase from thermococcus litoralis [Thermococcus litoralis],1K1X_B Crystal structure of 4-alpha-glucanotransferase from thermococcus litoralis [Thermococcus litoralis],1K1Y_A Crystal structure of thermococcus litoralis 4-alpha-glucanotransferase complexed with acarbose [Thermococcus litoralis],1K1Y_B Crystal structure of thermococcus litoralis 4-alpha-glucanotransferase complexed with acarbose [Thermococcus litoralis]
1K1W_A	3.34e-10	47	349	22	305	Crystalstructure of 4-alpha-glucanotransferase from thermococcus litoralis [Thermococcus litoralis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q59006	1.63e-93	13	400	9	398	Putative alpha-amylase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ1611 PE=3 SV=1
O57932	3.37e-11	47	301	22	268	Alpha-amylase OS=Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) OX=70601 GN=amyA PE=3 SV=1
O32462	7.83e-10	47	349	22	305	4-alpha-glucanotransferase OS=Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C) OX=523849 GN=jgt PE=1 SV=1
P49067	2.30e-08	47	213	23	184	Alpha-amylase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=amyA PE=1 SV=2
Q9V298	2.31e-08	47	301	22	268	Alpha-amylase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=amyA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000061	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003008_00440.