CAZyme Information: MGYG000002877_00323

Basic Information

• Species: Prevotella sp900550365
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella sp900550365
• CAZyme ID: MGYG000002877_00323
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
690	MGYG000002877_3|CGC2	76956.27	6.4363

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002877	2502849	MAG	United States	North America

• Gene Location: Start: 99595; End: 101667 Strand: +

Full Sequence      

MTTAAVALPAAAQHSGITDTRNSKYAVINSTPIDAVKWTGGFWGERFGVFSGTSLQSMWE
TWQSDEGKSFNNFLIAAGEKKGKRHGPPFHDGDMYKWLEAVAAVYAVNKDPELDRIMDRV
IGLIAKAQRPDGYLHTPVIIANLLKAGAQTSQIASDEAKENENIGTKVGTSGDGRFGNPL
NFEAYNMGHLITAGIIHKRATGKTTLFDCAVKAADCLYDFYMNPSKDALEKGVICPSHYM
AITEMYRETGNPKYLELSKQLINIRDSVKNGTDDNQDRIPFRQQYNAMGHSVRANYLYAG
VADLYVEDGEAQLKKNLDAIWDDIVYRKMYINGACGALYDGTSPDGTEYKPDLIQKVHQS
YGRPYQLPHSTAHNETCANIGNMLLNWRMFQATADAKYAGLVENCLYNSILCGISLDGTK
YFYTNPLRMSDELPYDLRWPNERMKYISCFCCPPNTLRTLCEAQDYAYSVGKGEIYVNLY
GQNTLTTKLDGIGEITLEQATDYPWNGNVALTVARLKGKKDFTLKLRIPEWCDNARITVN
GEPVNADIKSGSYAAITRRWKKGDVVTLDMPMDTKLMEANPLVEECRGQVAVQRGPILYC
IESNDLNGADIDNIAIPADAKFTPVETNIAGSRIMALETEAVDRGESKWTNTLYREAGKK
KNTVKIRLIPYYAWGNRGKSEMTVWIPVEL

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	36	600	4e-182	0.9980916030534351

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	0.0	35	600	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	3.56e-131	40	686	20	585	Uncharacterized conserved protein, DUF1680 family [Function unknown].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT38421.1	0.0	12	688	20	696
ALJ42161.1	0.0	12	688	20	696
QMW88025.1	0.0	12	688	20	696
QQA06605.1	0.0	12	688	20	696
AAO76110.1	0.0	12	688	20	696

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MQO_A	0.0	12	688	20	696	Glycosidehydrolase BT_1003 [Bacteroides thetaiotaomicron]
6EX6_A	7.20e-119	28	690	7	627	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
4QJY_A	6.07e-117	42	686	22	645	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	8.42e-113	42	686	22	645	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
7EXU_A	1.90e-82	72	685	56	655	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	2.21e-81	72	685	56	655	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000067	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002877_00323.