CAZyme Information: MGYG000002542_01032

Basic Information

• Species: Lentilactobacillus parafarraginis
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lentilactobacillus; Lentilactobacillus parafarraginis
• CAZyme ID: MGYG000002542_01032
• CAZy Family: GH25
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
314		35053.1	9.9941

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002542	2852137	Isolate	not provided	not provided

• Gene Location: Start: 19750; End: 20694 Strand: +

Full Sequence      

MIKRQIAYFLATVGVSVGIIASSAANTHAATLSIPDVSEWQGKITNLQAQNLKNQVSFVI
NRRQYGSSYVDKYATNNTALYVKYGIPFGEYDYAMFTSPASAKAEAKNFYNRSNKNARFY
VLDYEQNNVTSGSSKSAVSAWYNEMRSLTNKKLVFYSYQSFATQYAGSARNSFDAQWIAN
YSAKPSIHTDLWQYTNKHYLAALGKYLDDSKVMTSTHPVSWWTGNSDTATALQDAAITST
PKYYHALNTTKQLSTLKKVYLYNSKTFDKANRIKAVSAGSNISIQSVGKRASGSYYFTTT
TGQYVTANRSYVSE

Enzyme Prediction     

No EC number prediction in MGYG000002542_01032.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH25	36	200	8.4e-33	0.9717514124293786

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06523	GH25_PlyB-like	1.29e-59	34	212	2	177	PlyB is a bacteriophage endolysin that displays potent lytic activity toward Bacillus anthracis. PlyB has an N-terminal glycosyl hydrolase family 25 (GH25) catalytic domain and a C-terminal bacterial SH3-like domain, SH3b. Both domains are required for effective catalytic activity. Endolysins are produced by bacteriophages at the end of their life cycle and participate in lysing the bacterial cell in order to release the newly formed progeny. Endolysins (also referred to as endo-N-acetylmuramidases or peptidoglycan hydrolases) degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues.
cd00599	GH25_muramidase	6.09e-22	34	208	2	182	Endo-N-acetylmuramidases (muramidases) are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. This family of muramidases contains a glycosyl hydrolase family 25 (GH25) catalytic domain and is found in bacteria, fungi, slime molds, round worms, protozoans and bacteriophages. The bacteriophage members are referred to as endolysins which are involved in lysing the host cell at the end of the replication cycle to allow release of mature phage particles. Endolysins are typically modular enzymes consisting of a catalytically active domain that hydrolyzes the peptidoglycan cell wall and a cell wall-binding domain that anchors the protein to the cell wall. Endolysins generally have narrow substrate specificities with either intra-species or intra-genus bacteriolytic activity.
pfam01183	Glyco_hydro_25	1.53e-17	36	201	2	178	Glycosyl hydrolases family 25.
cd06524	GH25_YegX-like	1.32e-15	36	197	4	177	YegX is an uncharacterized bacterial protein with a glycosyl hydrolase family 25 (GH25) catalytic domain that is similar in sequence to the CH-type (Chalaropsis-type) lysozymes of the GH25 family of endolysins.
cd06522	GH25_AtlA-like	1.66e-14	36	210	5	189	AtlA is an autolysin found in Gram-positive lactic acid bacteria that degrades bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. This family includes the AtlA and Aml autolysins from Streptococcus mutans which have a C-terminal glycosyl hydrolase family 25 (GH25) catalytic domain as well as six tandem N-terminal repeats of the GBS (group B Streptococcus) Bsp-like peptidoglycan-binding domain. Other members of this family have one or more C-terminal peptidoglycan-binding domain(s) (SH3 or LysM) in addition to the GH25 domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QIR08946.1	8.39e-181	1	314	1	312
QHB51666.1	1.69e-180	1	314	1	312
QEU39757.1	2.08e-180	1	314	26	337
QGV25234.1	2.39e-120	4	312	9	306
AQW20679.1	2.01e-119	23	313	22	307

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2NW0_A	2.68e-22	34	227	3	188	ChainA, PlyB [Bacteriophage sp.],2NW0_B Chain B, PlyB [Bacteriophage sp.]
3HMC_A	2.08e-21	34	223	7	189	Endolysinfrom Bacillus anthracis [Bacillus anthracis]
4JZ5_A	6.34e-13	36	208	27	208	High-resolutionstructure of catalytic domain of endolysin ply40 from bacteriophage P40 of Listeria monocytogenes [Listeria phage P40]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000262	0.999080	0.000153	0.000198	0.000162	0.000150

TMHMM  Annotations     

start	end
7	29