CAZyme Information: MGYG000002527_03079

Basic Information

• Species: Aeromonas caviae
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Aeromonadaceae; Aeromonas; Aeromonas caviae
• CAZyme ID: MGYG000002527_03079
• CAZy Family: GH102
• CAZyme Description: Membrane-bound lytic murein transglycosylase A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
383	MGYG000002527_22|CGC5	41992.17	9.5645

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002527	4477475	Isolate	Brazil	South America

• Gene Location: Start: 478274; End: 479425 Strand: -

Full Sequence      

MNRHAYWLAAAVVGLAGCAGNPEPVKVPQKKAESGCFLNCEIPVNLGKQYLDGALPGDLV
RVERVNSRARADYSKFGPQSRMVMERSGRMASRYGELYRQLSRWVANGGNPAHITQYGIS
LAQLGGADRMGNVLFTGYYSPVLEVRHRPDAQYKYPIYAMPNCGGRCPSRAEIHRGALAN
RGLELGYSKSLIDNFLMDVQGSGFVHYGDDDRLQYLGYSGKNGHGYVSIGRVLIDRGEVP
KEQMSMKAIKEWANRQPEESVKELVEQNPSYVFFERRPTNDVVGAAGIPLLPMAAVAADK
TLLPMGTPILAEVPLLDKAGNWTGKHQLRLLIALDVGGAVKKGHLDLYHGMGEKAGVAAG
HYKHFGRVWKLGLHHGPTAAPWL

Enzyme Prediction     

No EC number prediction in MGYG000002527_03079.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH102	167	275	1.8e-39	0.6751592356687898

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK11162	mltA	0.0	34	372	19	355	murein transglycosylase A; Provisional
COG2821	MltA	9.66e-129	41	381	29	373	Membrane-bound lytic murein transglycosylase [Cell wall/membrane/envelope biogenesis].
smart00925	MltA	8.48e-63	142	274	1	152	MltA specific insert domain. This beta barrel domain is found inserted in the MltA a murein degrading transglycosylase enzyme. This domain may be involved in peptidoglycan binding.
pfam03562	MltA	3.36e-51	134	274	67	230	MltA specific insert domain. This beta barrel domain is found inserted in the MltA a murein degrading transglycosylase enzyme. This domain may be involved in peptidoglycan binding.
cd14668	mlta_B	8.56e-51	143	277	1	159	Domain B insert of mltA_like lytic transglycosylases. Escherichia coli MltA is a membrane-bound lytic transglycosylase comprised of two domains separated by a large groove, where the peptidoglycan strand binds. Domain A is made up of an N-terminal and a C-terminal portion, which correspond to the 3D domain, named for 3 conserved aspartate residues. Domain B is inserted within the linear sequence of domain A. MltA is distinct from other bacterial lytic transglycosylases (LTs), which are similar to each other. Escherichia coli peptidoglycan lytic transglycosylase (LT) initiates cell wall recycling in response to damage, during bacterial fission, and cleaves peptidoglycan (PG) to create functional spaces in its wall. PG chains (also known as murein), the major components of the bacterial cell wall, are comprised of alternating beta-1-4-linked N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), and lytic transglycosylases cleave this beta-1-4 bond. Typically, peptidoglycan lytic transglycosylases (LT) are exolytic, releasing Metabolite 1 (GlcNAc-anhMurNAc-L-Ala-D-Glu-m-Dap-D-Ala-D-Ala) from the ends of the PG strands. In contrast, MltE is endolytic , cleaving in the middle of PG strands, with further processing to Metabolite 1 accomplished by other LTs. In E. coli, there are six membrane-bound LTs: MltA-MltF and soluble Slt70. Slt35 is a soluble fragment cleaved from MltB. Bacterial LTs are classified in 4 families: Family 1 includes slt70 MltC-MltF, Family 2 includes MltA, Family 3 includes MltB, and Family 4 of bacteriophage origin. While most of the LT family members are similar in structure and sequence with a lysozyme-like fold, Family 2 (including mltA) is distinct.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUU21087.1	3.75e-290	1	383	1	383
ATP90723.1	3.75e-290	1	383	1	383
BDA13502.1	3.75e-290	1	383	1	383
BBQ30563.1	3.75e-290	1	383	1	383
QOK21032.1	3.75e-290	1	383	1	383

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2GAE_A	1.33e-105	43	371	6	334	Crystalstructure of MltA from E. coli [Escherichia coli]
2AE0_X	2.08e-104	43	371	5	333	Crystalstructure of MltA from Escherichia coli reveals a unique lytic transglycosylase fold [Escherichia coli]
2PJJ_A	3.36e-103	43	371	5	333	ChainA, Membrane-bound lytic murein transglycosylase A [Escherichia coli K-12]
2PIC_A	6.74e-103	43	371	5	333	ChainA, Membrane-bound lytic murein transglycosylase A [Escherichia coli K-12]
2PI8_A	9.10e-98	43	371	5	333	Crystalstructure of E. coli MltA with bound chitohexaose [Escherichia coli K-12],2PI8_B Crystal structure of E. coli MltA with bound chitohexaose [Escherichia coli K-12],2PI8_C Crystal structure of E. coli MltA with bound chitohexaose [Escherichia coli K-12],2PI8_D Crystal structure of E. coli MltA with bound chitohexaose [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9KPQ4	4.53e-149	43	382	21	360	Membrane-bound lytic murein transglycosylase A OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=mltA PE=3 SV=1
P0A935	1.35e-104	43	371	25	353	Membrane-bound lytic murein transglycosylase A OS=Escherichia coli (strain K12) OX=83333 GN=mltA PE=1 SV=1
P0A936	1.35e-104	43	371	25	353	Membrane-bound lytic murein transglycosylase A OS=Escherichia coli O157:H7 OX=83334 GN=mltA PE=3 SV=1
P57531	1.92e-80	42	371	25	356	Membrane-bound lytic murein transglycosylase A homolog OS=Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) OX=107806 GN=mltA PE=3 SV=1
Q8K9A7	5.82e-75	45	371	36	364	Membrane-bound lytic murein transglycosylase A homolog OS=Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) OX=198804 GN=mltA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000000	0.000136	0.999894	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002527_03079.