CAZyme Information: MGYG000002490_02156

Basic Information

• Species: Paenibacillus_B thiaminolyticus
• Lineage: Bacteria; Firmicutes; Bacilli; Paenibacillales; Paenibacillaceae; Paenibacillus_B; Paenibacillus_B thiaminolyticus
• CAZyme ID: MGYG000002490_02156
• CAZy Family: GH46
• CAZyme Description: Chitosanase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
299	MGYG000002490_23|CGC1	32962.26	9.5954

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002490	6537383	Isolate	Japan	Asia

• Gene Location: Start: 66622; End: 67521 Strand: +

Full Sequence      

MTNAKPAKKMTKLLVVLLSFSFLVSLSGISALLPTKAYAANSHDENFSPETLKFLRANTG
LDGEQWDNIMKLINKPEQDSLDWTKYYGYCEDIGDNRGYTIGIFGATTGGSNDKHPDGPA
LFKEFDAASGAANPSVEGGLARIGVNGSMNGSILKIKDSEKVFCGKINKLQNNDAWREAI
WQTFYKVYIKYSVQQARQRGFNSALTIGSFVDTALNQGATGGSGTLEGVLSRSGKSSDEK
TFMKKFYAERTKIVDTNDYNQPPNGKNRVKQWSQLWDMGKADLKNADDAIIKVTSWKMK

Enzyme Prediction     

EC	3.2.1.132

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH46	60	284	3.7e-75	0.9954954954954955

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd00978	chitosanase_GH46	1.41e-55	63	286	1	221	chitosanase belonging to the glycosyl hydrolase 46 family. This family is composed of the chitosanase enzymes which hydrolyzes chitosan, a biopolymer of beta (1,4)-linked-D-glucosamine (GlcN) residues produced by partial or full deacetylation of chitin. Chitosanases play a role in defense against pathogens such as fungi and are found in microorganisms, fungi, viruses, and plants. Microbial chitosanases can be divided into 3 subclasses based on the specificity of the cleavage positions for partial acetylated chitosan. Subclass I chitosanases such as N174 can split GlcN-GlcN and GlcNAc-GlcN linkages, whereas subclass II chitosanases such as Bacillus sp. no. 7-M can cleave only GlcN-GlcN linkages. Subclass III chitosanases such as MH-K1 chitosanase are the most versatile and can split both GlcN-GlcN and GlcN-GlcNAc linkages.
pfam01374	Glyco_hydro_46	7.77e-19	71	253	1	178	Glycosyl hydrolase family 46. This family are chitosanase enzymes.
cd16889	chitinase-like	6.73e-14	69	216	1	105	chitinase-like domain. This family includes proteins such as chitinases, chitosanase, pesticin, and endolysin, which are involved in the degradation of 1,4-N-acetyl D-glucosamine linkages in chitin polymers and related activities. Chitinases are enzymes that catalyze the hydrolysis of the beta-1,4-N-acetyl-D-glucosamine linkages in chitin polymers. Chitosanase enzymes hydrolyze chitosan, a biopolymer of beta (1,4)-linked-D-glucosamine (GlcN) residues produced by partial or full deacetylation of chitin. Pesticin (Pst) is a anti-bacterial toxin produced by Yersinia pestis that acts through uptake by the target related bacteria and the hydrolysis of peptidoglycan in the periplasm. The dsDNA phages of eubacteria use endolysins or muralytic enzymes in conjunction with hollin, a small membrane protein, to degrade the peptidoglycan found in bacterial cell walls. Similarly, bacteria produce autolysins to facilitate the biosynthesis of its cell wall heteropolymer peptidoglycan and cell division.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QDM46405.1	3.78e-218	1	299	1	299
SYX83235.1	9.24e-194	1	299	1	299
BAA01474.2	1.95e-154	1	299	3	301
BAA23489.1	1.65e-153	1	299	3	302
AUH49630.1	1.91e-153	37	299	44	306

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1QGI_A	3.55e-146	42	299	2	259	ChainA, Protein (chitosanase) [Niallia circulans]
2D05_A	3.55e-146	42	299	2	259	ChainA, Chitosanase [Niallia circulans]
5HWA_A	1.02e-145	42	299	2	259	ChainA, Chitosanase [Niallia circulans]
4ILY_A	2.48e-08	57	109	14	67	Abundantlysecreted chitosanase from Streptomyces sp. SirexAA-E [Streptomyces sp. SirexAA-E],4ILY_B Abundantly secreted chitosanase from Streptomyces sp. SirexAA-E [Streptomyces sp. SirexAA-E]
1CHK_A	6.01e-07	57	109	1	54	StreptomycesN174 Chitosanase Ph5.5 298k [Streptomyces sp. N174],1CHK_B Streptomyces N174 Chitosanase Ph5.5 298k [Streptomyces sp. N174]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P33673	3.90e-155	1	299	3	301	Chitosanase OS=Niallia circulans OX=1397 GN=csn PE=1 SV=2
P33665	1.76e-06	55	109	39	94	Chitosanase OS=Streptomyces sp. (strain N174) OX=69019 GN=csn PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000357	0.998845	0.000194	0.000218	0.000188	0.000162

TMHMM  Annotations     

start	end
13	35