CAZyme Information: MGYG000002477_00493

Basic Information

• Species: Citrobacter_A amalonaticus
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Citrobacter_A; Citrobacter_A amalonaticus
• CAZyme ID: MGYG000002477_00493
• CAZy Family: GT8
• CAZyme Description: General stress protein A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
337	MGYG000002477_2|CGC7	38803.16	6.037

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002477	5083867	Isolate	United States	North America

• Gene Location: Start: 426399; End: 427412 Strand: -

Full Sequence      

MTQTYFEEKVIQQTLDYNYEEHSDVDSFNIAYGIDKNFLFGCGVSIASILIANPDTPFSF
HVFTDSFSNEEQARFNALAKQYATQIVVYLVDCEQLKSLPSTKNWSYATYFRFIIADHFA
GKMDRVLYLDADIACQGSVQELIDLQFAEHEIAAVVAEGESEWWTKRSLSLATPGLVSGY
FNAGFLLINLPVWTTEDISRKAIEMLRDPDVVQRITHLDQDVLNMLLVGKARFIGKKFNT
QFSINYELKRDVVNPVDENTVFIHYIGPTKPWHQWGNYPIAQSFLNAKKLSPWCNVELLK
PNNSHQLRYAAKHMFNQKQYFSGLVDYALYFKSKIIK

Enzyme Prediction     

No EC number prediction in MGYG000002477_00493.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT8	1	275	1.7e-55	0.9727626459143969

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15171	PRK15171	0.0	5	336	3	334	lipopolysaccharide 3-alpha-galactosyltransferase.
COG1442	RfaJ	3.12e-93	28	337	3	319	Lipopolysaccharide biosynthesis protein, LPS:glycosyltransferase [Cell wall/membrane/envelope biogenesis].
pfam01501	Glyco_transf_8	7.34e-83	29	276	1	252	Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase.
cd04194	GT8_A4GalT_like	3.65e-76	28	273	1	247	A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS.
cd00505	Glyco_transf_8	4.04e-16	35	273	9	245	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AMG52032.1	7.68e-253	1	337	1	337
BCU46624.1	7.68e-253	1	337	1	337
QPB32476.1	6.31e-252	1	337	1	337
AUZ63968.1	1.81e-251	1	337	1	337
QZA36548.1	1.81e-251	1	337	1	337

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1SS9_A	1.52e-21	29	276	2	260	ChainA, alpha-1,4-galactosyl transferase [Neisseria meningitidis]
1G9R_A	2.09e-21	29	276	2	260	ChainA, GLYCOSYL TRANSFERASE [Neisseria meningitidis],1GA8_A Chain A, GALACTOSYL TRANSFERASE LGTC [Neisseria meningitidis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P19816	2.61e-197	1	337	1	337	Lipopolysaccharide 1,3-galactosyltransferase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=rfaI PE=3 SV=4
P27128	1.29e-134	1	335	1	337	Lipopolysaccharide 1,3-galactosyltransferase OS=Escherichia coli (strain K12) OX=83333 GN=rfaI PE=3 SV=1
P19817	4.89e-68	22	335	20	333	Lipopolysaccharide 1,2-glucosyltransferase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=rfaJ PE=3 SV=4
P27129	2.27e-56	26	325	26	325	Lipopolysaccharide 1,2-glucosyltransferase OS=Escherichia coli (strain K12) OX=83333 GN=rfaJ PE=3 SV=2
P43974	6.64e-23	27	274	38	280	Putative glycosyltransferase HI_0258 OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=HI_0258 PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000030	0.000008	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002477_00493.