CAZyme Information: MGYG000002470_03260

Basic Information

• Species: Bacteroides intestinalis
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides intestinalis
• CAZyme ID: MGYG000002470_03260
• CAZy Family: GH127
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
801	MGYG000002470_5|CGC6	89702.49	7.2089

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002470	5774847	Isolate	Ireland	Europe

• Gene Location: Start: 310228; End: 312633 Strand: -

Full Sequence      

MRKVLVTTLLVTATVGSQAQVKNQSHGYPIDPVPFTSVKVTDSFWGQRLNASREVTIPLA
FSKCEETGRYQNFVNAAHPSDTIKVGGLAFDDTDVYKTIEGASYLLQTYPDKKLAKYIDS
VLVIVAAAQEPDGYLYTSRTMNPKHPHEWAGSKRWEKVEDLSHEFYNLGHMVEGAIAHYQ
ATGKKNFLNIAIRYADCVCREIGTGEGQQIRVPGHQIAEMALAKLYLVTGDKKYLDQAKF
FLDQRGYTSRTDEYSQAHKPVVQQDEAVGHAVRAAYMYAGMADVAALTGDTAYIHAIDRI
WDNIVGKKYYITGGIGATAAGEAFGKNYELPNMSAYCETCAAIGNVYVNYRLFLLHGESK
YYDVLERTLYNGLISGVSLDGGGFFYPNPLESMGQHQRQPWFGCACCPSNICRFIPSLPG
YIYAVKDKDVYVNLFMSNTSDLKVGGKAVSIEQTTQYPWNGDITIGINKNSAGQFNLKVR
IPGWVRGQVVPSDLYTYSDGKRLKYTVKVNGEAVQNELKDGYFCIDRRWKKGDKVEVHFD
MEPRIVKANNKVEADRGRIAVERGPIVYCAEFPDNNFDIFSVFMNRNPKFEVVEKPDLLY
GINQLKTGAQTLGYDDQGRLTTTDVSLTLIPYYAWAHRGSGAMEVWLPQELSASRPAMPA
TLASESKIDASHRAKSISAINDRLIPKDENDRSLPYYHWWPKQGTTEWITYEFPAEAAVS
SSTVYWFDDAPWGGCRVPKSWKIYYKDAQGQWQPVTGADKYGVVKGAGNTVNFDPVKTKS
IKLEITLPDKHAAGVYEWEVQ

Enzyme Prediction     

No EC number prediction in MGYG000002470_03260.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	38	569	2.6e-210	0.9980916030534351

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	0.0	37	569	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	7.50e-174	29	650	2	588	Uncharacterized conserved protein, DUF1680 family [Function unknown].
COG1331	YyaL	6.80e-04	221	369	419	564	Uncharacterized conserved protein YyaL, SSP411 family, contains thoiredoxin and six-hairpin glycosidase-like domains [General function prediction only].
cd04791	LanC_SerThrkinase	0.006	179	314	99	243	Lanthionine synthetase C-like domain associated with serine/threonine kinases. Some members of this subgroup lack the zinc binding site and the active site residues, and therefore are most likely inactive. The function of this domain is unknown.
cd00249	AGE	0.009	169	302	176	332	AGE domain; N-acyl-D-glucosamine 2-epimerase domain; Responsible for intermediate epimerization during biosynthesis of N-acetylneuraminic acid. Catalytic mechanism is believed to be via nucleotide elimination and readdition and is ATP modulated. AGE is structurally and mechanistically distinct from the other four types of epimerases. The AGE domain monomer is composed of an alpha(6)/alpha(6)-barrel, the structure of which is also found in glucoamylase and cellulase. The active form is a homodimer. The alignment also contains subtype III mannose 6-phosphate isomerases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QDO67740.1	0.0	1	801	1	801
ALJ59249.1	0.0	1	801	1	801
QUT89692.1	0.0	1	801	1	801
QJR66040.1	0.0	1	801	1	801
QJR70733.1	0.0	1	801	1	801

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6EX6_A	0.0	25	654	2	630	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
4QJY_A	5.24e-149	36	650	14	648	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	2.18e-144	36	650	14	648	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	4.26e-110	37	646	2	655	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	5.72e-110	37	646	2	655	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	2.34e-109	37	646	2	655	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1
E8MGH9	1.62e-06	662	799	1150	1298	Beta-L-arabinobiosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000438	0.998793	0.000229	0.000200	0.000168	0.000158

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002470_03260.