CAZyme Information: MGYG000002436_02976

Basic Information

• Species: Pigmentiphaga sp002188635
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Burkholderiales; Burkholderiaceae; Pigmentiphaga; Pigmentiphaga sp002188635
• CAZyme ID: MGYG000002436_02976
• CAZy Family: GH13
• CAZyme Description: Maltooligosyl trehalose synthase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
895	MGYG000002436_35|CGC1	98427.89	6.6451

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002436	5862145	Isolate	Canada	North America

• Gene Location: Start: 61327; End: 64014 Strand: +

Full Sequence      

MNGSVFPARAAGQGIVACGVPRATARLQFHAGFPFEAAAAQVGYFRDLGISHLYASPITR
ARPGSMHGYDTVDYGCVSPELGGEEGLRRLVARLREHGMGLIVDIVPNHMGVGKENAWWW
SVLKLGRASPYAEFFDIDWEPADLALCGKVLAPFLGEQYGQVLAAGQLSVRLDEETGEPV
IAYFDARFPIAPDCAAEIAAQPPEAYDATRPEGRARLHALLERQHYRLAWWRTAAEEINW
RRFFEVSDLIGVRVEREDTREAIHALPFRLYAEGLIDGVRVDHIDGLADPEAYCRYLREN
LAALDGGRPAGCPRDVPYIVVEKILASDEPLPASWRVQGTTGYDFMNDVGALLHHPEGEA
RLTAQWEAVSGEARGFRALVLDVRRRLVAQSFAGECDALVRILHRIARTDLATRDWGAPA
IRRVLTELLVHFPVYRTYVGGETPLAADEVAMGIACKGARQVLRRADHGLLELLAQWLGQ
APDAPATPALRAWRREALRRFQQLTAPLAAKSVEDTGFYRYGRLLSRNEVGSDPGVFSLD
AEGFHRKVLARAARFPDAMLATATHDHKRGEDARARLAVLSEFGDEWLRLVGAWMRRHEA
LKAREGPRPAPADEYMLYQTLFGAWPLDLAIADEAGIAALAERVGEWQTKSLREAKLRSS
WVTPADEYEQACDAFLRAVLDPRRNGRSLLELSAFVARAGAAGALNGLAQTLLRLSTPGV
PDLYQGTEFWDFSLVDPDNRRPVDYGRRRAALASDADCASLVANWRDGHIKFELIRRALA
LRQRDPDLFRRGDYLPLAAHGDARDAVLAFARRHEDRFAVAIVPLHASALVLASGPSPVP
HVPPGAWGDTSIDLSGLVRDGALRNVLDGRSCAVAGGTVRVADALAAFPVALMHA

Enzyme Prediction     

No EC number prediction in MGYG000002436_02976.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	42	351	9e-126	0.9898648648648649

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK14507	PRK14507	0.0	7	895	729	1690	malto-oligosyltrehalose synthase.
cd11336	AmyAc_MTSase	0.0	23	784	1	659	Alpha amylase catalytic domain found in maltooligosyl trehalose synthase (MTSase). Maltooligosyl trehalose synthase (MTSase) domain. MTSase and maltooligosyl trehalose trehalohydrolase (MTHase) work together to produce trehalose. MTSase is responsible for converting the alpha-1,4-glucosidic linkage to an alpha,alpha-1,1-glucosidic linkage at the reducing end of the maltooligosaccharide through an intramolecular transglucosylation reaction, while MTHase hydrolyzes the penultimate alpha-1,4 linkage of the reducing end, resulting in the release of trehalose. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase.
COG3280	TreY	0.0	20	895	3	888	Maltooligosyltrehalose synthase [Carbohydrate transport and metabolism].
PRK14511	PRK14511	0.0	20	894	4	876	malto-oligosyltrehalose synthase.
TIGR02401	trehalose_TreY	0.0	21	893	1	823	malto-oligosyltrehalose synthase. This enzyme, formally named (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase, is the TreY enzyme of the TreYZ pathway of trehalose biosynthesis, an alternative to the OtsAB pathway. Trehalose may be incorporated into more complex compounds but is best known as compatible solute. It is one of the most effective osmoprotectants, and unlike the various betaines does not require nitrogen for its synthesis. [Energy metabolism, Biosynthesis and degradation of polysaccharides]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AZG08126.1	0.0	14	895	11	892
QDZ27380.1	9.66e-315	20	893	3	933
ANN77576.1	1.32e-310	21	893	4	896
ALM82504.1	6.08e-301	7	895	6	907
BBK43530.1	1.33e-300	21	893	4	856

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6LCV_A	3.20e-135	20	893	1	754	structureof Mutant S44P of maltooligosyltrehalose synthase from Arthrobacter ramosus [Arthrobacter ramosus]
6LCU_A	4.82e-134	20	893	1	754	structureof maltooligosyltrehalose synthase from Arthrobacter ramosus [Arthrobacter ramosus]
5ZCR_A	3.07e-99	24	797	5	664	DSM5389glycosyltrehalose synthase [Saccharolobus shibatae B12],5ZCR_B DSM5389 glycosyltrehalose synthase [Saccharolobus shibatae B12]
1IV8_A	7.33e-92	23	812	4	669	CrystalStructure of Maltooligosyl trehalose synthase [Sulfolobus acidocaldarius]
3HJE_A	2.56e-86	35	797	14	646	Crystalstructure of sulfolobus tokodaii hypothetical maltooligosyl trehalose synthase [Sulfurisphaera tokodaii str. 7]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q44315	1.23e-149	21	893	4	767	Maltooligosyl trehalose synthase OS=Arthrobacter sp. (strain Q36) OX=104027 GN=treY PE=1 SV=1
P9WQ20	2.80e-148	32	861	21	744	Putative maltooligosyl trehalose synthase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=treY PE=3 SV=1
P9WQ21	2.80e-148	32	861	21	744	Putative maltooligosyl trehalose synthase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=treY PE=1 SV=1
Q9R9H8	6.61e-11	41	137	183	275	Intracellular maltogenic amylase OS=Bacillus subtilis OX=1423 GN=bbmA PE=1 SV=2
O06988	6.61e-11	41	137	183	275	Intracellular maltogenic amylase OS=Bacillus subtilis (strain 168) OX=224308 GN=bbmA PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000062	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002436_02976.