CAZyme Information: MGYG000002435_00508

Basic Information

• Species: Campylobacter_A concisus
• Lineage: Bacteria; Campylobacterota; Campylobacteria; Campylobacterales; Campylobacteraceae; Campylobacter_A; Campylobacter_A concisus
• CAZyme ID: MGYG000002435_00508
• CAZy Family: GT66
• CAZyme Description: Undecaprenyl-diphosphooligosaccharide--protein glycotransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
706	MGYG000002435_16|CGC1	81589.65	8.4864

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002435	1884438	Isolate	Denmark	Europe

• Gene Location: Start: 17888; End: 20008 Strand: +

Full Sequence      

MHKVSVNCKILLIFLVAYLFGFAARMLWVLWAKDMPEFYFNGEFMLTTNDAYYYAEGARD
MLAGFHQQNDFSPFNHPISTIVFYICKVFPFKIESVMFYMSALLAPLIALPVILISNELK
ALKAGAVAAFMSVILPSYLSRTSPGYFDSDMLNVTFALFIIYFLIRLLNTNEQKFIVLPG
VFVSLYLWWYQSSYALILSIFFMFLLYTLVFMRDRLQNYQAIFFMFISIVSSNVFTKDPL
IANKIFVFNLVVIALFFSLFCKYLLSARNLAIFLTLMLAIFIYFGGFDFITSKIGIYVFK
SNEILSDKFHFINEHNFISEVKSSSPLYFIYFMSGNILILMAAIVGYLLLCFKFRPFLLT
LPMLGLGLLSFFSGVRFVMYVTPLVALGFGYFLHFFLNLFDLRKSIKNLSFLVFAVAALA
INLDFAYSYRPKTVISRDEAVALDGLKKVTKRDDYVFSWWDYGYAIRYFADVMTLNDPGR
QGGENNYFVSLALRKDEAFSARLARVAVAYNDISLEQNIRPVDKILKDYNTSDINTFLSQ
LESENFTLPAAKKDVFYYLMPNMIDIAPNIFRYSYIDITTGKRQKENFYHVSTLNGVSEA
SIDLGDGYTLPTNDQKFIIHNGEKIAVKSFYKVKGTGRDLRIDEKIIDENAKIYVVFLED
YARILLLDENAFNSSFVQLFIFERADDRYFEPFIISNGVKIYRLKI

Enzyme Prediction     

No EC number prediction in MGYG000002435_00508.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT66	12	592	9.8e-93	0.9062049062049062

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1287	Stt3	5.27e-32	10	494	22	538	Asparagine N-glycosylation enzyme, membrane subunit Stt3 [Posttranslational modification, protein turnover, chaperones].
pfam02516	STT3	2.19e-29	10	479	3	477	Oligosaccharyl transferase STT3 subunit. This family consists of the oligosaccharyl transferase STT3 subunit and related proteins. The STT3 subunit is part of the oligosaccharyl transferase (OTase) complex of proteins and is required for its activity. In eukaryotes, OTase transfers a lipid-linked core-oligosaccharide to selected asparagine residues in the ER. In the archaea STT3 occurs alone, rather than in an OTase complex, and is required for N-glycosylation of asparagines.
TIGR04154	archaeo_STT3	3.00e-19	11	490	1	566	oligosaccharyl transferase, archaeosortase A system-associated. Members of this protein family occur, one to three members per genome, in the same species of Euryarchaeota as contain the predicted protein-sorting enzyme archaeosortase (TIGR04125) and its cognate protein-sorting signal PGF-CTERM (TIGR04126).
pfam18527	STT3_PglB_C	1.12e-05	597	666	10	79	STT3/PglB C-terminal beta-barrel domain. Asparagine-linked glycosylation is a post-translational modification of proteins containing the conserved sequence motif Asn-X-Ser/Thr. The attachment of oligosaccharides is implicated in diverse processes such as protein folding and quality control, organism development or host-pathogen interactions. The reaction is catalysed by oligosaccharyltransferase (OST), a membrane protein complex located in the endoplasmic reticulum. The central, catalytic enzyme of OST is the STT3 subunit, which has homologues in bacteria and archaea. Structural analysis of a bacterial OST, undecaprenyl-diphosphooligosaccharide protein glycotransferase EC:2.4.99.19 (PglB) protein, revealed two domains: a transmembrane domain and a periplasmic domain. This entry represents the C-terminal periplasmic beta-barrel domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ALF48124.1	0.0	1	706	1	706
QPI01678.1	0.0	1	706	1	708
QPH99886.1	0.0	1	706	1	708
QPI03445.1	0.0	1	706	1	708
QPH88497.1	0.0	1	706	1	708

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3RCE_A	2.10e-119	2	705	11	711	Bacterialoligosaccharyltransferase PglB [Campylobacter lari]
5OGL_A	1.98e-116	12	705	18	711	Structureof bacterial oligosaccharyltransferase PglB in complex with an acceptor peptide and an lipid-linked oligosaccharide analog [Campylobacter lari RM2100]
6GXC_A	2.64e-116	12	705	18	711	Bacterialoligosaccharyltransferase PglB in complex with an inhibitory peptide and a reactive lipid-linked oligosaccharide analog [Campylobacter lari RM2100]
3AAG_A	2.36e-34	433	706	9	291	Crystalstructure of C. jejuni pglb C-terminal domain [Campylobacter jejuni RM1221],3AAG_B Crystal structure of C. jejuni pglb C-terminal domain [Campylobacter jejuni RM1221]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q5HTX9	5.65e-120	15	706	19	713	Undecaprenyl-diphosphooligosaccharide--protein glycotransferase OS=Campylobacter jejuni (strain RM1221) OX=195099 GN=pglB PE=1 SV=1
Q0P9C8	7.94e-120	17	706	21	713	Undecaprenyl-diphosphooligosaccharide--protein glycotransferase OS=Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) OX=192222 GN=pglB PE=1 SV=1
B9KDD4	4.24e-119	2	705	11	711	Undecaprenyl-diphosphooligosaccharide--protein glycotransferase OS=Campylobacter lari (strain RM2100 / D67 / ATCC BAA-1060) OX=306263 GN=pglB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999373	0.000592	0.000028	0.000002	0.000001	0.000039

TMHMM  Annotations     

start	end
10	32
93	115
151	168
175	190
194	211
218	235
245	267
269	291
328	350
357	374
378	400
409	428