Species | Robinsoniella peoriensis | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Lineage | Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Robinsoniella; Robinsoniella peoriensis | |||||||||||
CAZyme ID | MGYG000002403_05504 | |||||||||||
CAZy Family | GH4 | |||||||||||
CAZyme Description | Alpha-galacturonidase | |||||||||||
CAZyme Property |
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Genome Property |
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Gene Location | Start: 126264; End: 127646 Strand: - |
Family | Start | End | Evalue | family coverage |
---|---|---|---|---|
GH4 | 12 | 191 | 4.8e-57 | 0.9888268156424581 |
Cdd ID | Domain | E-Value | qStart | qEnd | sStart | sEnd | Domain Description |
---|---|---|---|---|---|---|---|
cd05297 | GH4_alpha_glucosidase_galactosidase | 1.80e-145 | 11 | 448 | 1 | 423 | Glycoside Hydrolases Family 4; Alpha-glucosidases and alpha-galactosidases. linked to 3D####ucture |
COG1486 | CelF | 1.22e-97 | 11 | 460 | 4 | 442 | Alpha-galactosidase/6-phospho-beta-glucosidase, family 4 of glycosyl hydrolase [Carbohydrate transport and metabolism]. |
PRK15076 | PRK15076 | 1.17e-64 | 11 | 448 | 2 | 420 | alpha-galactosidase; Provisional |
pfam02056 | Glyco_hydro_4 | 5.09e-60 | 12 | 191 | 1 | 180 | Family 4 glycosyl hydrolase. |
cd05197 | GH4_glycoside_hydrolases | 3.59e-47 | 11 | 433 | 1 | 409 | Glycoside Hydrases Family 4. Glycoside hydrolases cleave glycosidic bonds to release smaller sugars from oligo- or polysaccharides. Some bacteria simultaneously translocate and phosphorylate disaccharides via the phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). After translocation, these phospho-disaccharides may be hydrolyzed by GH4 glycoside hydrolases. Other organisms (such as archaea and Thermotoga maritima) lack the PEP-PTS system, but have several enzymes normally associated with the PEP-PTS operon. GH4 family members include 6-phospho-beta-glucosidases, 6-phospho-alpha-glucosidases, alpha-glucosidases/alpha-glucuronidases (only from Thermotoga), and alpha-galactosidases. They require two cofactors, NAD+ and a divalent metal (Mn2+, Ni2+, Mg2+), for activity. Some also require reducing conditions. GH4 glycoside hydrolases are part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others. |
Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End |
---|---|---|---|---|---|
AFK85236.1 | 3.39e-196 | 1 | 456 | 1 | 459 |
SNX53414.1 | 1.71e-193 | 1 | 460 | 1 | 463 |
ANX00783.1 | 2.34e-193 | 6 | 455 | 6 | 457 |
AGC67847.1 | 4.71e-193 | 6 | 455 | 6 | 457 |
ANW98258.1 | 4.71e-193 | 6 | 455 | 6 | 457 |
Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
---|---|---|---|---|---|---|
3FEF_A | 9.71e-149 | 9 | 449 | 4 | 437 | Crystalstructure of putative glucosidase lplD from bacillus subtilis [Bacillus subtilis],3FEF_B Crystal structure of putative glucosidase lplD from bacillus subtilis [Bacillus subtilis],3FEF_C Crystal structure of putative glucosidase lplD from bacillus subtilis [Bacillus subtilis],3FEF_D Crystal structure of putative glucosidase lplD from bacillus subtilis [Bacillus subtilis] |
1OBB_A | 2.01e-25 | 11 | 421 | 4 | 439 | alpha-glucosidaseA, AglA, from Thermotoga maritima in complex with maltose and NAD+ [Thermotoga maritima MSB8],1OBB_B alpha-glucosidase A, AglA, from Thermotoga maritima in complex with maltose and NAD+ [Thermotoga maritima MSB8] |
1S6Y_A | 4.84e-23 | 5 | 433 | 2 | 416 | 2.3Acrystal structure of phospho-beta-glucosidase [Geobacillus stearothermophilus] |
5C3M_A | 1.57e-22 | 79 | 433 | 75 | 413 | Crystalstructure of Gan4C, a GH4 6-phospho-glucosidase from Geobacillus stearothermophilus [Geobacillus stearothermophilus],5C3M_B Crystal structure of Gan4C, a GH4 6-phospho-glucosidase from Geobacillus stearothermophilus [Geobacillus stearothermophilus],5C3M_C Crystal structure of Gan4C, a GH4 6-phospho-glucosidase from Geobacillus stearothermophilus [Geobacillus stearothermophilus],5C3M_D Crystal structure of Gan4C, a GH4 6-phospho-glucosidase from Geobacillus stearothermophilus [Geobacillus stearothermophilus] |
6VC6_A | 4.90e-16 | 13 | 434 | 6 | 417 | 2.1Angstrom Resolution Crystal Structure of 6-phospho-alpha-glucosidase from Gut Microorganisms in Complex with NAD and Mn2+ [Merdibacter massiliensis],6VC6_B 2.1 Angstrom Resolution Crystal Structure of 6-phospho-alpha-glucosidase from Gut Microorganisms in Complex with NAD and Mn2+ [Merdibacter massiliensis],6VC6_C 2.1 Angstrom Resolution Crystal Structure of 6-phospho-alpha-glucosidase from Gut Microorganisms in Complex with NAD and Mn2+ [Merdibacter massiliensis],6VC6_D 2.1 Angstrom Resolution Crystal Structure of 6-phospho-alpha-glucosidase from Gut Microorganisms in Complex with NAD and Mn2+ [Merdibacter massiliensis] |
Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
---|---|---|---|---|---|---|
I3VRU1 | 6.78e-197 | 1 | 456 | 1 | 459 | Alpha-galacturonidase OS=Thermoanaerobacterium saccharolyticum (strain DSM 8691 / JW/SL-YS485) OX=1094508 GN=Tsac_0200 PE=1 SV=1 |
D3T426 | 1.56e-180 | 1 | 456 | 1 | 451 | Alpha-galacturonidase OS=Thermoanaerobacter italicus (strain DSM 9252 / Ab9) OX=580331 GN=Thit_1733 PE=1 SV=1 |
A9KTB9 | 4.66e-173 | 1 | 455 | 1 | 460 | Alpha-galacturonidase OS=Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) OX=357809 GN=Cphy_3396 PE=1 SV=1 |
P39130 | 8.24e-154 | 9 | 449 | 8 | 441 | Alpha-galacturonidase OS=Bacillus subtilis (strain 168) OX=224308 GN=lplD PE=1 SV=2 |
O34645 | 6.82e-52 | 12 | 458 | 3 | 431 | Alpha-galactosidase OS=Bacillus subtilis (strain 168) OX=224308 GN=melA PE=1 SV=1 |
Other | SP_Sec_SPI | LIPO_Sec_SPII | TAT_Tat_SPI | TATLIP_Sec_SPII | PILIN_Sec_SPIII |
---|---|---|---|---|---|
1.000078 | 0.000000 | 0.000000 | 0.000000 | 0.000000 | 0.000000 |
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