CAZyme Information: MGYG000002369_03013

Basic Information

• Species: Clostridioides difficile
• Lineage: Bacteria; Firmicutes_A; Clostridia; Peptostreptococcales; Peptostreptococcaceae; Clostridioides; Clostridioides difficile
• CAZyme ID: MGYG000002369_03013
• CAZy Family: GH1
• CAZyme Description: 6-phospho-beta-glucosidase BglA

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
484	MGYG000002369_1|CGC31	56070.31	4.7521

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002369	4190038	Isolate	United States	North America

• Gene Location: Start: 3354760; End: 3356214 Strand: -

Full Sequence      

MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPRVITDKIEEKLDYPSHN
GIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPNELGIKYYEGLIDTLIENN
IEPIVTLYHFELPMNLVHKYGSWNNRKLMDLYLKYSETVIRRFDDKVKYWITFNEMNHID
PQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLAGVKVAKLAREINPNNKVGCVFG
LNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKFPQYKLKEYEKNNITIEVTDEDKKA
FKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGGIQNPYLEQSKWGWAIDPIGLRFTLNY
IYRKYQLPILITENGLGAYDKIEEDGTINDDYRIEYLSKHLSQMKSAIEEDNVECFGYLM
WGPIDLVSATTGQMSKRYGFIYVDLDDEGKGSFKRYKKKSFDWYKEVIKSNGEVLDFSTD
IVVG

Enzyme Prediction     

No EC number prediction in MGYG000002369_03013.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH1	4	472	1.4e-136	0.9836829836829837

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK09589	celA	0.0	1	475	2	476	6-phospho-beta-glucosidase; Reviewed
COG2723	BglB	0.0	7	476	8	458	Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase [Carbohydrate transport and metabolism].
PRK15014	PRK15014	5.51e-179	7	475	10	477	6-phospho-beta-glucosidase BglA; Provisional
PRK09593	arb	2.31e-172	1	476	4	478	6-phospho-beta-glucosidase; Reviewed
PRK09852	PRK09852	3.10e-169	5	476	6	474	cryptic 6-phospho-beta-glucosidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AYD19027.1	0.0	1	484	1	484
AVB35093.1	0.0	1	484	1	484
AYD15384.1	0.0	1	484	1	484
AXB62194.1	0.0	1	484	1	484
QGS41029.1	0.0	1	484	1	484

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2XHY_A	1.83e-172	1	475	6	479	CrystalStructure of E.coli BglA [Escherichia coli K-12],2XHY_B Crystal Structure of E.coli BglA [Escherichia coli K-12],2XHY_C Crystal Structure of E.coli BglA [Escherichia coli K-12],2XHY_D Crystal Structure of E.coli BglA [Escherichia coli K-12]
4F66_A	2.61e-157	3	473	7	478	Thecrystal structure of 6-phospho-beta-glucosidase from Streptococcus mutans UA159 in complex with beta-D-glucose-6-phosphate. [Streptococcus mutans],4F66_B The crystal structure of 6-phospho-beta-glucosidase from Streptococcus mutans UA159 in complex with beta-D-glucose-6-phosphate. [Streptococcus mutans]
4F79_A	7.42e-157	3	473	7	478	Thecrystal structure of 6-phospho-beta-glucosidase mutant (E375Q) in complex with Salicin 6-phosphate [Streptococcus mutans],4GPN_A The crystal structure of 6-P-beta-D-Glucosidase (E375Q mutant) from Streptococcus mutans UA150 in complex with Gentiobiose 6-phosphate. [Streptococcus mutans UA159],4GPN_B The crystal structure of 6-P-beta-D-Glucosidase (E375Q mutant) from Streptococcus mutans UA150 in complex with Gentiobiose 6-phosphate. [Streptococcus mutans UA159]
6WGD_A	6.72e-155	3	475	8	469	Crystalstructure of a 6-phospho-beta-glucosidase from Bacillus licheniformis [Bacillus licheniformis],6WGD_B Crystal structure of a 6-phospho-beta-glucosidase from Bacillus licheniformis [Bacillus licheniformis],6WGD_C Crystal structure of a 6-phospho-beta-glucosidase from Bacillus licheniformis [Bacillus licheniformis]
3PN8_A	8.98e-153	3	473	7	478	Thecrystal structure of 6-phospho-beta-glucosidase from Streptococcus mutans UA159 [Streptococcus mutans],3PN8_B The crystal structure of 6-phospho-beta-glucosidase from Streptococcus mutans UA159 [Streptococcus mutans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q46829	1.00e-171	1	475	6	479	6-phospho-beta-glucosidase BglA OS=Escherichia coli (strain K12) OX=83333 GN=bglA PE=1 SV=2
P42973	2.64e-164	2	475	3	479	Aryl-phospho-beta-D-glucosidase BglA OS=Bacillus subtilis (strain 168) OX=224308 GN=bglA PE=1 SV=1
P40740	1.98e-156	3	475	8	469	Aryl-phospho-beta-D-glucosidase BglH OS=Bacillus subtilis (strain 168) OX=224308 GN=bglH PE=1 SV=2
Q46130	4.55e-143	1	476	5	472	6-phospho-beta-glucosidase OS=Clostridium longisporum OX=1523 GN=abgA PE=3 SV=1
P24240	9.86e-140	3	476	4	474	6-phospho-beta-glucosidase AscB OS=Escherichia coli (strain K12) OX=83333 GN=ascB PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000031	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002369_03013.