CAZyme Information: MGYG000002310_02009

Basic Information

• Species: Microvirga massiliensis
• Lineage: Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Beijerinckiaceae; Microvirga; Microvirga massiliensis
• CAZyme ID: MGYG000002310_02009
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
963	MGYG000002310_3|CGC2	106072.47	6.9375

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002310	8892798	Isolate	not provided	not provided

• Gene Location: Start: 138075; End: 140966 Strand: +

Full Sequence      

MSGILVKLFATALTMSQVAIQPDNVRTSFDAVQDRGEVDWILKEGCAHMRRAFDIEEINL
DELITTAMDDPDALTGNLKVLQGLSFSDLHASYRQFCTTEQVERSPVDLGEVITYFNEAV
ANLPDAGKLKGLRLPGVTVILDQTGKRFAEIYEPDHRRMWVPLSEIPKPVQQAFVAAEDK
RFYEHKGIDERGVIRAFMSNLTNPGRPAGGSTITQQVVKNLLVGDDVTYERKMREMIVAA
RVERILSKDEILEIYLNAIYFGRSSWGIEMAARSYFGKPAKELSLAEGALLAGLVKGPNV
YNPDRSPDRALDRVAYVLSRMEEDKFVTAGQITLLLGKLPPTIPFERPQRSSGFHFVDFL
DRSARPVAGIGSLTGSGYTVRSTINPALQKATEAALQEGLARYELNTGRSRFQGAESNLA
EAIAKLSADDTPSNSDKASPPWRRALEAARLPLYDVQWPAAIVIEKSVPRKGALTFRVGL
ADGSTMPLTVASADARRALKLYDVVRVRVIPASGKKAARAELRMPPSVQGAAVVLENRTG
RILAMSGGFSYPLSQLNRVTQAHRQPGSALKPLTYLAALAKGLQPNTLVWDEPVTFPPIG
GNAGARASDSWTPKNYDGRSGGIMTLRRALETSQNLVTARLLEGGLDWDPERSLRSVCEL
ALEAQLYVECEPYYPFVLGAQPVRLLDLAAFYAAITTEGALPSPYALEAVEEGGHTIYRR
ENIAPVQIGSADRVAFYQLKTILQGVLERGTARSIRTLAPYVAGKTGTSDHENDAWFVGF
TNDVTVAVWVGYDNADGKRRTLGRGQTGGKIAVPIFQAIIEAAWAHHALKAPLNPPSPQA
ARQLIAMPIDLETGDRITDGGRKAFTEHFRLSQFGQFTDTQFRLVPEYDAYAFRRPGPWS
DGDEAPLGGYFDDAPVAEAPGWFESVPPTARGRTHDLPWWEEDRPRRSRRIDPDYFWGNG
RIY

Enzyme Prediction     

No EC number prediction in MGYG000002310_02009.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	145	322	7.5e-68	0.9887005649717514

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG5009	MrcA	0.0	122	869	33	758	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	1.71e-154	157	823	1	528	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	2.30e-144	108	823	27	596	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
PRK11636	mrcA	5.58e-89	123	861	33	815	penicillin-binding protein 1a; Provisional
TIGR02071	PBP_1b	2.54e-86	146	794	134	659	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRM31909.1	0.0	1	963	1	964
ANY80498.1	0.0	1	963	1	970
ANV99652.1	0.0	1	957	1	948
QHO74087.1	0.0	1	957	1	950
AWN45061.1	0.0	1	963	1	997

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3UDF_A	6.13e-81	161	816	42	708	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
4OON_A	2.00e-79	129	870	12	763	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
5U2G_A	2.14e-67	117	865	1	793	2.6Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20],5U2G_B 2.6 Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20]
7U4H_A	3.32e-48	121	869	5	801	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]
5FGZ_A	9.67e-48	157	794	155	670	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q68VU2	8.53e-106	122	860	33	747	Penicillin-binding protein 1A OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=mrcA PE=3 SV=1
Q9ZCE9	1.73e-105	122	860	33	747	Penicillin-binding protein 1A OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=mrcA PE=3 SV=1
Q92G78	1.89e-104	122	861	33	747	Penicillin-binding protein 1A OS=Rickettsia conorii (strain ATCC VR-613 / Malish 7) OX=272944 GN=mrcA PE=3 SV=1
Q1RKC5	3.74e-104	112	861	26	747	Penicillin-binding protein 1A OS=Rickettsia bellii (strain RML369-C) OX=336407 GN=mrcA PE=3 SV=1
Q4UK08	3.74e-101	122	861	33	747	Penicillin-binding protein 1A OS=Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) OX=315456 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.270008	0.728817	0.000431	0.000267	0.000231	0.000239

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002310_02009.