CAZyme Information: MGYG000002298_00518

Basic Information

• Species: Blautia_A sp000436615
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia_A; Blautia_A sp000436615
• CAZyme ID: MGYG000002298_00518
• CAZy Family: GT35
• CAZyme Description: Glycogen phosphorylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
755	MGYG000002298_1|CGC5	84931.55	4.9599

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002298	4216146	Isolate	China	Asia

• Gene Location: Start: 600408; End: 602675 Strand: -

Full Sequence      

MSETMTNKLIELCGKDIRTASNAELYHALLKLVDEKSQEQVQNVTGRKLYYISAEFLIGK
LLSNNLINLGLYDDVKEALKEAGKSLADIEEQEPEPSLGNGGLGRLAACFLDSLATLNLP
GDGVGLRYHCGLFRQNFKNNVQNETPDFWLTDSCAAKATDTVFPVSLAGKEYSARLYKLP
VTGYEGRTNTLNLFDLDSVDESVIGDGISFDKKDIARNLTLFLYPDDSDEDGRLLRIYQQ
YFMVSAGAQLILSECAARGCNYHDLVDYAAIQINDTHPSMVIPELIRLLMLHDIDFEEAV
QIVTDTCAYTNHTILAEALEKWPRHYLDTVVPQLMPIIEKLDALAKERTKDASLAIIDQN
QVVHMAHMDIHFSHSTNGVATLHTQILKESELAGFYHLYPEKFNNKTNGITFRRWLLKCN
PTLTSEIESLIGSGFKKDASELKKLLAYTDDVDVLKKISEIKEQNKQALATWLERKQGVK
INTDAMFTIQSKRLHEYKRQQLNLLFLIHQYLEIKAGHIPSVPLVSIFGAKAAPAYIIAK
DIIHGLLTLSQVIENDPEVSKWMQLVFVENYNVSAAEMMIPACDLSEQISLASKEASGTG
NMKFMLNGALTLGTMDGANVEISEAVGNDNIYIFGQSSKQVIHRYAAGDYCSRDWYEADP
NLHRAIDFLTGKEMLSFGDEEHLKRLQNELIGKDWFQTLPDFNAYVVRKQQAIADYAADQ
ENWSRRTLINIAEAGFFSSDRTIAEYDNDIWHLGK

Enzyme Prediction     

EC	2.4.1.1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT35	78	753	1.3e-222	0.9970326409495549

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK14986	PRK14986	0.0	8	753	24	810	glycogen phosphorylase; Provisional
COG0058	GlgP	0.0	8	755	16	750	Glucan phosphorylase [Carbohydrate transport and metabolism].
PRK14985	PRK14985	0.0	47	751	59	794	maltodextrin phosphorylase; Provisional
TIGR02093	P_ylase	0.0	5	753	1	794	glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources. [Energy metabolism, Biosynthesis and degradation of polysaccharides]
cd04300	GT35_Glycogen_Phosphorylase	0.0	7	753	6	795	glycogen phosphorylase and similar proteins. This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBL23581.1	0.0	1	755	1	755
ADD61666.1	0.0	5	755	8	758
CCG34911.1	0.0	4	755	2	753
AXB27758.1	0.0	4	755	2	753
CBL01526.1	0.0	4	755	2	753

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4L22_A	4.58e-303	11	752	14	756	Crystalstructure of putative glycogen phosphorylase from Streptococcus mutans [Streptococcus mutans UA159]
2C4M_A	1.89e-181	14	753	22	788	Starchphosphorylase: structural studies explain oxyanion-dependent kinetic stability and regulatory control. [Corynebacterium callunae],2C4M_B Starch phosphorylase: structural studies explain oxyanion-dependent kinetic stability and regulatory control. [Corynebacterium callunae],2C4M_C Starch phosphorylase: structural studies explain oxyanion-dependent kinetic stability and regulatory control. [Corynebacterium callunae],2C4M_D Starch phosphorylase: structural studies explain oxyanion-dependent kinetic stability and regulatory control. [Corynebacterium callunae]
5OX0_A	3.64e-172	15	751	42	826	GlycogenPhosphorylase in complex with CK898 [Oryctolagus cuniculus]
2GJ4_A	4.16e-172	15	751	30	814	Structureof rabbit muscle glycogen phosphorylase in complex with ligand [Oryctolagus cuniculus]
2FFR_A	4.28e-172	15	751	30	814	Crystallographicstudies on N-azido-beta-D-glucopyranosylamine, an inhibitor of glycogen phosphorylase: comparison with N-acetyl-beta-D-glucopyranosylamine [Oryctolagus cuniculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P29849	1.71e-313	14	753	13	751	Maltodextrin phosphorylase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=malP PE=3 SV=2
P39123	1.23e-179	13	753	20	794	Glycogen phosphorylase OS=Bacillus subtilis (strain 168) OX=224308 GN=glgP PE=2 SV=1
P0AC86	1.28e-177	1	753	17	810	Glycogen phosphorylase OS=Escherichia coli (strain K12) OX=83333 GN=glgP PE=3 SV=1
P0AC87	1.28e-177	1	753	17	810	Glycogen phosphorylase OS=Shigella flexneri OX=623 GN=glgP PE=3 SV=1
Q3B7M9	4.53e-173	15	751	42	826	Glycogen phosphorylase, brain form OS=Bos taurus OX=9913 GN=PYGB PE=2 SV=3

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000057	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002298_00518.