CAZyme Information: MGYG000002273_04536

Basic Information

• Species: Bacteroides stercorirosoris
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides stercorirosoris
• CAZyme ID: MGYG000002273_04536
• CAZy Family: CBM38
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
934	MGYG000002273_45|CGC1	105903.78	5.9795

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002273	6091524	Isolate	China	Asia

• Gene Location: Start: 33319; End: 36123 Strand: -

Full Sequence      

MNFDTPLGPWKVCYQTFEPASYIDQGFKITVNDGSKTVTKELNKKDFDDIAFIGEYPIAF
IEYGHKNENLPVKVSAEVYSPFVPLDAKKSATPATIMKYTVKNVSDRKVTANLEGWMQNL
VCIDLKGRAEGMLQNKVIDNNGWKSVYMDMRVDNLPIKTSDKSRKYEVFENFESGTYNRW
KVEGTAFGRKPSPGEQCNQNPFGGDYGNYLANSYTNYDKPVGKLTSKPFTIKHKFISFNI
AGGSHPGKTCINLVIDGKVAMSATGNNNEDFLPRNWDVSKYKGKKAYLEIVDSVEGDWGH
ISVDNIEFSNSPVAAPATTIDKSHAYFGNLALSVFDNNATATADDSKNNVEYAETELGNK
INGALSSSMTLQPGESKEVTFVLSWYFPNRPLSYKGSEWNKPLPPNSPAIGNMYSNWYDS
SLDVAEQLHENYAELAQETHNFHNTYYNETNLPYWLVQRVMMPISTLATETCQWWATDKF
WAWEGVGSCEGTCTHVWGYAQSMAALFPELERNLREKTDYSVSLREDGAILSRNGEHGVL
IDGHASVILRMYREHLMSKNNFFLSRNWDKIKRSLQYIINEDGNEDGLIVKTQPNTYDIS
FNGANTYVGALYLAALRAGEKMAYIMKDTVAANYYKKIYTAGSENTMDRLWNGEYFYQDV
DEKEYPKYQYGKGCLSDQLFGQTWASLYRLGDIYPTDAINKALRSIWKFNWTNDVKAQSE
AHLPERYYAHAGEPGLFICTWPYSKHPGEDGVRYRDEVWTGIEYQVATEMIKRDMTDEGL
TIVKAIHSRYDGKKHNPWNEIECGDHYARAMASYGVLQAIQGYWYDGPKGIMGFEPKLRK
DDFIGFFNAAEGWGNISQIRKNNKQTNCVEVKFGRLYLDKLQVNLAEGTDINNIKASLNN
KNIVVSATNTSGYTEIALNGLQMRSGDKLQITFE

Enzyme Prediction     

No EC number prediction in MGYG000002273_04536.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH116	541	816	2.5e-65	0.7272727272727273
CBM38	171	293	5.4e-36	0.9612403100775194

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam04685	DUF608	2.71e-44	542	816	99	362	Glycosyl-hydrolase family 116, catalytic region. This represents a family of archaeal, bacterial and eukaryotic glycosyl hydrolases, that belong to superfamily GH116. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335, with the likely acid/base at Asp-442 and the aspartates at Asp-406 and Asp-458 residues also playing a role in the catalysis of glucosides and xylosides that are beta-bound to hydrophobic groups. The family is defined as GH116, which presently includes enzymes with beta-glucosidase, EC:3.2.1.21, beta-xylosidase, EC:3.2.1.37, and glucocerebrosidase EC:3.2.1.45 activity.
COG4354	COG4354	7.23e-38	97	823	3	720	Uncharacterized protein, contains GBA2_N and DUF608 domains [Function unknown].
pfam12215	Glyco_hydr_116N	5.07e-11	52	435	81	309	beta-glucosidase 2, glycosyl-hydrolase family 116 N-term. This domain is found in bacteria, archaea and eukaryotes. This domain is typically between 320 to 354 amino acids in length. This domain is found associated with pfam04685. It is found just after the extreme N-terminus. The N-terminal is thought to be the luminal domain while the C terminal is the cytosolic domain. The catalytic domain of GBA-2 is unknown. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335 in the downstream family pfam04685.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QDO68264.1	0.0	1	934	100	1033
QUT37401.1	0.0	1	934	100	1033
QBJ18459.1	0.0	1	934	100	1033
ASV73565.1	5.12e-248	14	876	86	952
QDV74336.1	3.80e-235	17	931	148	1065

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5FJS_A	3.42e-27	213	798	143	753	Bacterialbeta-glucosidase reveals the structural and functional basis of genetic defects in human glucocerebrosidase 2 (GBA2) [Thermoanaerobacterium xylanolyticum LX-11],5FJS_B Bacterial beta-glucosidase reveals the structural and functional basis of genetic defects in human glucocerebrosidase 2 (GBA2) [Thermoanaerobacterium xylanolyticum LX-11],5O0S_A Crystal structure of txGH116 (beta-glucosidase from Thermoanaerobacterium xylolyticum) in complex with unreacted beta Cyclophellitol Cyclosulfate probe ME711 [Thermoanaerobacterium xylanolyticum LX-11]
5BVU_A	3.51e-27	213	798	147	757	Crystalstructure of Thermoanaerobacterium xylolyticum GH116 beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],5BX2_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with 2-deoxy-2-fluoroglucoside [Thermoanaerobacterium xylanolyticum LX-11],5BX3_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with deoxynojirimycin [Thermoanaerobacterium xylanolyticum LX-11],5BX4_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with Glucoimidazole [Thermoanaerobacterium xylanolyticum LX-11],5BX5_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with glucose [Thermoanaerobacterium xylanolyticum LX-11],5NCX_A Crystal structure of Thermoanaerobacterium xylolyticum GH116 beta-glucosidase with an covalent inhibitor [Thermoanaerobacterium xylanolyticum LX-11],5OST_A Beta-glucosidase from Thermoanaerobacterium xylolyticum GH116 in complex with Gluco-1H-imidazole [Thermoanaerobacterium xylanolyticum LX-11]
5NPF_A	3.51e-27	213	798	146	756	Crystalstructure of txGH116 (beta-glucosidase from Thermoanaerobacterium xylolyticum) in complex with beta Cyclophellitol Cyclosulfate probe ME594 [Thermoanaerobacterium xylanolyticum]
7W2V_A	3.83e-27	213	798	190	800	ChainA, Glucosylceramidase [Thermoanaerobacterium xylanolyticum LX-11]
7W2W_A	3.83e-27	213	798	190	800	ChainA, Glucosylceramidase [Thermoanaerobacterium xylanolyticum LX-11],7W2X_A Chain A, Glucosylceramidase [Thermoanaerobacterium xylanolyticum LX-11]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
K0E4E1	2.24e-24	166	311	316	461	Putative glycosyl hydrolase ecdE OS=Aspergillus rugulosus OX=41736 GN=ecdE PE=3 SV=1
Q9HCG7	3.57e-12	542	779	619	841	Non-lysosomal glucosylceramidase OS=Homo sapiens OX=9606 GN=GBA2 PE=1 SV=2
Q69ZF3	4.66e-12	542	779	610	832	Non-lysosomal glucosylceramidase OS=Mus musculus OX=10090 GN=Gba2 PE=1 SV=2
Q5M868	8.03e-12	542	779	610	832	Non-lysosomal glucosylceramidase OS=Rattus norvegicus OX=10116 GN=Gba2 PE=2 SV=2
Q7KT91	5.52e-11	563	778	687	890	Non-lysosomal glucosylceramidase OS=Drosophila melanogaster OX=7227 GN=CG33090 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000075	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002273_04536.