CAZyme Information: MGYG000002267_03750

Basic Information

• Species: Eisenbergiella sp003478085
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Eisenbergiella; Eisenbergiella sp003478085
• CAZyme ID: MGYG000002267_03750
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1184	MGYG000002267_17|CGC2	134652.18	5.3237

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002267	7234430	Isolate	China	Asia

• Gene Location: Start: 124871; End: 128425 Strand: +

Full Sequence      

MRWGKLFNDGWEFAKQVLTETKNEEVPSVFVPVEIPHDWLIYDSYNLYQDSIGWYRKSFM
HEKKPGKQYFLRFDGIYMDSTIYINGNTACEWKYGYSSFVLDVTGFLKEGVNEILVKAVY
HSPNSRWYSGAGIYRNIWFQEVDPVWLIQDGIYISEREEQNGNWRVTVSAEIGVKQQTDP
RDITLRFQVFKRRGNTNKNTAGMDTVYSSSQFSNYEEDITDGQEPIAECLAGAAVIQEKK
GGSDNGLFYGTAQLMIDSPDLWSPDHPCLYIVKTELHKGGKIVQTEYTRFGFRTFAYKPD
SGFWLNGRNIKIKGVCEHHDLGCLGAAYNQTAMRRKLKILKEMGVNAVRTAHNMPAAEWM
DLADEMGILIDSEGFDMWEKPKTTYDYARFFTEWHCRDIASWIRRDRNHPSVIMWSIGNE
IYDTHEGERGLQITKELSDEVRKHDPAGNAPVTIGSNYMPWENAQKCADIVKLAGYNYSE
KYYDAHHKEHPDWIIYGSETASIVQSRGIYHFPYSKSILADEDEQCSSLGNSRTSWGAVS
TEMCIAGERDNHFSCGQFIWTGFDYIGEPTPYHTKNSYFGQIDTAGFPKDSYYIYQAEWQ
EAKKDVLHLFPYWDFNEGQTVDVRAASNAGWLELFINGKSQGIRKIDHVHGTILTGDWSV
TYEPGEIRVKAYDDKKTLVAEAFHKSFQDAAEIRCSADRESALADGRDLLFVEISMVDKD
GHAVENANNRVRVDVSGSGRLAGLDNGDSTDFDSYKGNDRRLFGGKLLAVIQTKTVPGKI
TVTVSSYGLPDQELILWARECREKFEVIESDLPTGEGKRTDKTDVPVRKIELLMNGSRKL
DKACPQAEITAKLYPENAVDTVLFWSVVDDAGIPSNLAQISVNGKHAVVKAKGDGCFRVR
CMSRNGTEKIRLISQLDFEVTGIGTAYLNPYEFVCGGLYQYSKGEVGNGNEYGVATARDG
ETQVGYRELDFGPVGSDEITLPVFALSDEPYRIRIWEGMPGEEGSIQIGDVLYQKPSRWN
VYQEETYHLDKKLKGITSICFVVDKKIHIKGFIFTRQNPAYDCICAAHCKHVYGDHFKVE
RDGIYDIGNNVTLEFPQMDFGEDGLQGIAICGSSPIEKNTIHIRFENQETGEEIQQIVEF
GQSSGMDVRTYRLDRVKGRQNVYFIFLPGSRFDMKWFRFLPDKT

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	6	604	4e-106	0.6023936170212766

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	3.01e-61	7	779	15	791	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam18565	Glyco_hydro2_C5	7.60e-40	693	794	1	102	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK10150	PRK10150	1.86e-33	50	471	62	464	beta-D-glucuronidase; Provisional
PRK10340	ebgA	2.57e-25	53	420	111	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam02836	Glyco_hydro_2_C	1.43e-23	300	506	5	208	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QLG36749.1	0.0	5	1179	5	1157
QZN78011.1	0.0	5	1179	5	1160
APO44079.1	0.0	5	1179	5	1155
AZK49101.1	0.0	6	1179	6	1147
AVV58790.1	0.0	6	1180	6	1151

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7CWD_A	4.94e-173	7	806	6	809	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	1.95e-165	7	789	12	798	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
5T98_A	2.76e-148	4	789	27	811	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
6QUB_B	7.61e-147	7	789	12	844	Truncatedbeta-galactosidase III from Bifidobacterium bifidum in complex with galactose [Bifidobacterium bifidum]
6QUB_A	1.62e-146	7	789	35	867	Truncatedbeta-galactosidase III from Bifidobacterium bifidum in complex with galactose [Bifidobacterium bifidum],6QUC_A Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum],6QUC_B Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum],6QUD_A 2-deoxy-galactose reaction intermediate of a Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	2.87e-162	33	800	74	850	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	1.19e-92	7	786	51	808	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	4.64e-48	8	680	57	702	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	1.62e-39	7	792	8	731	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KN75	1.82e-37	7	790	31	797	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000088	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002267_03750.