CAZyme Information: MGYG000002256_00855

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; UBA3282
• CAZyme ID: MGYG000002256_00855
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase large subunit

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
639	MGYG000002256_16|CGC1	74334.23	4.7854

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002256	2755304	MAG	Peru	South America

• Gene Location: Start: 13937; End: 15856 Strand: +

Full Sequence      

MKPDIRWLDDPQVFRAGQLPAHSDHEIYADPQEMDRGESSLYQSLDGMWDFQYSVNAKSR
PQDFFSEGFDASGFDRIQVPGHIELAGYDKIHYINTMYPWEGTIFRRPAYAFGEDRKEEG
SFSEAEYNPVGSYRRRFDLEQGLLGKRVCICFEGVEQAMYVWLNGHFIGYAEDSFTPSEF
DLTPYIRGKGNLLAVEVHKRSTAAFLEDQDFFRFFGIFRSVRLYAKPKAHVEDLWIRPYL
HRDNASGELKVSVQLSVADETAGAVRVRAALLDAQKKERTVKEMDWQPKDGGNGPGLEFD
WEEQDVTPWSNGNPYLYTLQLELIRENKVLEAVSCPVGFRRIEIEDKVIKLNGERLIICG
VNRHEWSAEGGRCISVEDMKWDIDCMKRNRINAVRTCHYPDRREWYDLCDRAGIYVMAET
NLESHGSWQKMGAIEPSWNVPGSVPQWKEAVTDRARTNFACFKNHPSILFWSLGNESFAG
DDIAEMNRLFKEQDDLRLTHYEGVYHTPSYKDRISDVESRMYAYPQEIIDYMENDPQKPY
LLCEYMHDMGNSLGGMNSYMELIDRYEMFQGGFIWDYMDQALYVTDEVTGKRVLRYGGDF
DDRPSDYEFSGNGILFADRTEKPAMQEVRYYYGIKNRSR

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	24	632	4.4e-150	0.6316489361702128

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10340	ebgA	3.32e-158	6	629	3	596	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	1.92e-141	11	630	20	623	beta-galactosidase.
pfam02836	Glyco_hydro_2_C	1.86e-132	342	633	1	298	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
COG3250	LacZ	5.18e-123	39	636	9	557	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	5.20e-43	133	476	69	417	beta-D-glucuronidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNM02889.1	0.0	1	639	1	639
ABX42393.1	4.90e-307	1	634	1	637
AQP40966.1	1.78e-305	1	634	1	631
VCV22603.1	5.58e-302	1	634	1	631
CBL13227.1	5.58e-302	1	634	1	631

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6S6Z_A	5.66e-135	9	632	5	593	Structureof beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_B Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_C Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_D Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_E Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_F Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_G Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_H Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8]
6SD0_A	5.78e-135	9	632	6	594	Structureof beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_B Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_C Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_D Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8]
3DEC_A	1.01e-106	18	632	17	613	ChainA, Beta-galactosidase [Bacteroides thetaiotaomicron VPI-5482]
1F4A_A	3.06e-102	9	630	15	620	E.COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4A_B E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4A_C E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4A_D E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4H_A E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli],1F4H_B E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli],1F4H_C E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli],1F4H_D E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli]
5A1A_A	3.12e-102	9	630	16	621	2.2A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12],5A1A_B 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12],5A1A_C 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12],5A1A_D 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q48846	2.33e-295	1	633	1	622	Beta-galactosidase large subunit OS=Latilactobacillus sakei OX=1599 GN=lacL PE=3 SV=1
Q02603	1.55e-274	1	634	1	624	Beta-galactosidase large subunit OS=Leuconostoc lactis OX=1246 GN=lacL PE=1 SV=1
Q7WTB4	5.18e-271	1	634	1	626	Beta-galactosidase large subunit OS=Lactobacillus helveticus OX=1587 GN=lacL PE=2 SV=1
O07684	4.04e-263	1	634	1	626	Beta-galactosidase large subunit OS=Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) OX=272621 GN=lacL PE=3 SV=2
P24131	4.93e-212	2	632	5	612	Beta-galactosidase OS=Clostridium acetobutylicum OX=1488 GN=cbgA PE=2 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000062	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002256_00855.