CAZyme Information: MGYG000002247_01926

Basic Information

• Species: Acetatifactor sp900771995
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Acetatifactor; Acetatifactor sp900771995
• CAZyme ID: MGYG000002247_01926
• CAZy Family: CBM27
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1483	MGYG000002247_33|CGC1	161162.8	4.2527

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002247	3138747	MAG	Peru	South America

• Gene Location: Start: 32766; End: 37217 Strand: -

Full Sequence      

MKNKLRVGALLLTAVMLASSVYESAPIVVRAEEVVTQTVSDGNAEGVVKEAETSEMVQVY
ENGKDITFDNTKDWDDKGEFNIALTTGEALNRGATVTMDVLVPEDAAYKGIIKIQGVARI
GSNWTWTEADSIAELTIDSFGKAENGYRKAAVTFTFGAGVEADALAQFTLKLAGYQCDYS
GAVYIANVVLTDGEKQENGGVQGETTDVLVAEQADRQQVYGKEIAFDPSSNWNDQGEFEI
GITTEEALHSGAYLTMDVLVPENAQFANIIKVQGVAKVGDSWTWTEAANIPELKIDSFGE
AADGYRSATIRFDFGGNIEAGAVKTMLIKLAGYQCDYKGSVYIENIRLYDKQSSGETPMP
EKDPSIIDDFEDGTCDDWWNGGAWQYDNSIVNTVVEYNGSKMMKVDLDYTGMGSYSWSEA
KLENKFADSFDISAYNLLSFDYYYPAAMSGSKIKIFSNSGINSEAEITEEEILADGMIRA
KVQVKFSPTDKGLADLTIGFVGVNTEFVGSVYVDNITLSQYNAAGDYVKVTATPNADGTQ
ADISHAPTQVRLSDANAGASATALYAYLMALRESDQVLFGHENDINKMVSSTATEGDVRE
ITGSVSGLYGIDSLALTGAELGIADANAALEQAIAYSKQAAEQGAIITLSTHMPNFTNEK
IKVNADGSYNFTACDFSEAKDTSNNCAEQILPGGACNDRFTAYLDIIAEYANALQQDNIP
VLFRPYHENTGSWFWWGSSTSVETYKSLWRYTQEYLQSKGVHNLIYVYSPNGPLSGAEEY
LTRYPGDEYVDVLAFDYYDDYSTYPATFDDSFFKNLKTTCQVVSDLAAEKGKVAAISETG
VRAMKKDGSDYEGLLVTGNPIVGHNWYQQVSGIAAECDMPYWLVWANFGDTNFYVPYKYN
DTYGQELINEFIEFYNSDNSIFGNGTNFYGKMGSVEGTTYDTAQGYLIAPFELAAIKEET
LFRASVKNAENVSFVVKNPVNGKEITVEAAKVEGSIREYAAAFTAQMLQEIGQTDTATVS
LVADGKVLAIVKNISLGKDKDKAAANVIDNFDYYADSDGLLQSSYTENSAGGCSSSFTLS
SDHKADGAYGGAFHYHLSTAKSEVWTGQNKTLEDIDLSAYNAINLWVDPDGTGQKLVIQL
ADDSGEEFEVYLSDFVKGTEASYVTIPFSSFKGKQNGTLNTAKITRFAIWCNSIIPEGHS
GDWTVDSVIYFDGIEAFTAEEALLAQADANGLIFTKESPIKREDPTPEDSKDNSTASDSG
SSTSSAEEITVNWTEVQAQTTVAKAKDAGGNLNFVSIGETKVPAAVLNSIRGSKVTIAFH
SGNGLALSISGQSLKNVDLTGLQNIDLTAKREGDYITVKDTGSFAVPVNMHVAMGRENAG
KYANLYRRNTESGQWEYCGSFRITEAGQAMFALSCGGEYRVSATAEVVKETVVFAGGSYV
VKAGDNLSKIAAKHRISLKELIRKNPQIKDINKIRIGQTIRIR

Enzyme Prediction     

No EC number prediction in MGYG000002247_01926.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH26	553	886	4.6e-58	0.900990099009901
CBM23	1047	1208	7.9e-43	0.9753086419753086
CBM27	371	518	1.4e-25	0.9583333333333334

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam02156	Glyco_hydro_26	1.37e-39	554	810	2	243	Glycosyl hydrolase family 26.
COG4124	ManB2	4.16e-18	705	896	169	352	Beta-mannanase [Carbohydrate transport and metabolism].
cd00118	LysM	4.73e-11	1438	1482	2	45	Lysin Motif is a small domain involved in binding peptidoglycan. LysM, a small globular domain with approximately 40 amino acids, is a widespread protein module involved in binding peptidoglycan in bacteria and chitin in eukaryotes. The domain was originally identified in enzymes that degrade bacterial cell walls, but proteins involved in many other biological functions also contain this domain. It has been reported that the LysM domain functions as a signal for specific plant-bacteria recognition in bacterial pathogenesis. Many of these enzymes are modular and are composed of catalytic units linked to one or several repeats of LysM domains. LysM domains are found in bacteria and eukaryotes.
smart00257	LysM	6.92e-10	1439	1482	2	44	Lysin motif.
pfam01476	LysM	8.40e-09	1439	1482	1	42	LysM domain. The LysM (lysin motif) domain is about 40 residues long. It is found in a variety of enzymes involved in bacterial cell wall degradation. This domain may have a general peptidoglycan binding function. The structure of this domain is known.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBK83362.1	0.0	85	1247	265	1453
EEV00397.1	0.0	270	1240	116	1086
VCV20359.1	0.0	270	1240	104	1074
CBL14297.1	0.0	270	1240	104	1074
CBL07467.1	0.0	270	1240	104	1074

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4YN5_A	6.46e-53	551	936	50	431	Catalyticdomain of Bacillus sp. JAMB-750 GH26 Endo-beta-1,4-mannanase [Bacillus sp. JAMB750]
1R7O_A	1.60e-48	532	887	3	334	CrystalStructure of apo-mannanase 26A from Psudomonas cellulosa [Cellvibrio japonicus]
1J9Y_A	4.23e-48	547	887	6	324	Crystalstructure of mannanase 26A from Pseudomonas cellulosa [Cellvibrio japonicus]
2WHM_A	1.94e-47	547	887	6	324	Cellvibriojaponicus Man26A E121A and E320G double mutant in complex with mannobiose [Cellvibrio japonicus]
1GW1_A	2.11e-47	547	887	2	320	Substratedistortion by beta-mannanase from Pseudomonas cellulosa [Cellvibrio japonicus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A1A278	1.41e-48	550	1208	40	696	Mannan endo-1,4-beta-mannosidase OS=Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a) OX=367928 GN=BAD_1030 PE=1 SV=1
P49424	1.70e-47	532	887	31	362	Mannan endo-1,4-beta-mannosidase OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=manA PE=1 SV=2
Q5AWB7	4.85e-16	608	851	76	313	Probable mannan endo-1,4-beta-mannosidase E OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=manE PE=3 SV=1
P49425	5.79e-15	508	800	105	379	Mannan endo-1,4-beta-mannosidase OS=Rhodothermus marinus (strain ATCC 43812 / DSM 4252 / R-10) OX=518766 GN=manA PE=1 SV=3
P16699	3.49e-14	552	798	34	270	Mannan endo-1,4-beta-mannosidase A and B OS=Caldalkalibacillus mannanilyticus (strain DSM 16130 / CIP 109019 / JCM 10596 / AM-001) OX=1236954 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000295	0.999003	0.000191	0.000189	0.000151	0.000136

TMHMM  Annotations     

start	end
7	29