CAZyme Information: MGYG000002239_00632

Basic Information

• Species: UMGS1613 sp900553395
• Lineage: Bacteria; Firmicutes; Bacilli; RF39; UBA660; UMGS1613; UMGS1613 sp900553395
• CAZyme ID: MGYG000002239_00632
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 2D

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
650		74378.17	4.9778

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002239	719902	MAG	Peru	South America

• Gene Location: Start: 3192; End: 5144 Strand: -

Full Sequence      

MKKMKKCLKLFTFFIVILTFLYLGIYLVASLTTKLDINSSNGYYLFDSNNNLINGTSDQW
IKLNDISDNLIKATIAIEDKNFYRHQGFDYLRIAKSLYVNLVNRKTLQGASTITQQYAKN
LFLEFDKTWERKAKEAWLTIRLESQYSKDEILEGYLNTINYGGVYGIENASYYYFNKSAS
ELTLAEASILAGIPKSPKNYSPILNYDKSKERQYLVLNAMVENKYITREEMDKAYKEELT
FFGFIENNNLKTLRYFEDAVMDELNTLDLPSSFLETGGIKIYTTLDMNAQTILENSINDN
FTIEEMQLSSIILNPNNGEVLALAGGKDYSKSQFNRALYAKRQVGSTLKPFLYYVALENG
FTESTTFTSEETTFVFSNNQTYSPSNYNNKYGNKDITMAAAISYSENIYAVKTHLFLGED
TLVEMLKRVGITSKLEAIPSLALGSEEITLFDMTNAYATLASGGYKNKPHFIKRIEDSNG
KILYEFKDEKEQVLNSSLVYIMNEILTSTYNYNFIDYSYPTCYDIASKLTNKFSVKTGTT
DTDHLVFGYNKDIVVGIWSGYDDNRKTEAKNGKQIKMMWTDIVENYFKDKETSWYQLPNN
VVGVLVDPISGKVADENTSKPTMFYYIKGTEPTYSDSSLDNLIPTIKEEK

Enzyme Prediction     

No EC number prediction in MGYG000002239_00632.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	56	221	1.3e-63	0.9378531073446328

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02074	PBP_1a_fam	0.0	59	589	3	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	2.00e-180	11	582	17	592	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	1.14e-142	61	649	70	781	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
pfam00912	Transgly	1.80e-80	59	221	14	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.
PRK11636	mrcA	6.58e-73	61	635	69	831	penicillin-binding protein 1a; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AZV45469.1	1.25e-180	39	632	59	653
QOR66557.1	1.93e-178	55	650	71	671
ASV67977.1	2.00e-178	55	633	71	654
AEH49664.1	5.99e-178	54	650	70	672
QYF83258.1	7.25e-178	35	632	49	653

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5U2G_A	2.08e-58	61	633	43	801	2.6Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20],5U2G_B 2.6 Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20]
5FGZ_A	3.35e-54	54	568	152	675	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]
3VMA_A	4.22e-54	54	568	173	696	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli K-12]
3UDF_A	7.14e-54	61	489	42	564	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
7U4H_A	7.73e-54	29	633	10	808	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P70997	1.67e-163	55	632	71	653	Penicillin-binding protein 2D OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpG PE=2 SV=3
O66874	1.02e-78	60	643	65	723	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
Q92G78	3.36e-77	60	618	69	748	Penicillin-binding protein 1A OS=Rickettsia conorii (strain ATCC VR-613 / Malish 7) OX=272944 GN=mrcA PE=3 SV=1
Q9ZCE9	1.62e-75	60	618	69	748	Penicillin-binding protein 1A OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=mrcA PE=3 SV=1
Q4UK08	8.75e-75	60	618	69	748	Penicillin-binding protein 1A OS=Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) OX=315456 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.770030	0.225466	0.001248	0.000545	0.000385	0.002323

TMHMM  Annotations     

start	end
7	29