CAZyme Information: MGYG000002218_01924

Basic Information

• Species: Parabacteroides sp000436495
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tannerellaceae; Parabacteroides; Parabacteroides sp000436495
• CAZyme ID: MGYG000002218_01924
• CAZy Family: GH97
• CAZyme Description: Retaining alpha-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
661	MGYG000002218_17|CGC1	75210.79	4.76

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002218	3613635	MAG	Spain	Europe

• Gene Location: Start: 18781; End: 20766 Strand: +

Full Sequence      

MVQKSLLTLVSLLVLSLGNVLNAKEYVVSSPDKAIQMKLVVEEDISYDVMYHGKTLMKQN
QIALELSNGILGQSPVVKKVKQKAFQEHVDCFNYRCPSFDVSYNEMYVTFKGDYALVFRV
YNDGVAYRWETKMKEDIVVRNEQADFNWAEDYVVYLPFTTSKKDPYAMAFQNIYAVSKIS
EADNSKVAFLPVTADCGEGVKVTILESDLENYPGMFVLADPKARALKADFAEYPEEYAVN
STRAMKYVTKRSDDIARCQGTRSFPWRIMAITSDDTQMPVNNLVYALASPNRIGDYSWVK
SGKVAWDWWNDWGIWNVDFKAGINMDTYKHYIDFAAENKIGYVVLDEGWYNPKSGDMLTV
IPELNLEELIAYGKSKDVDLILWTVFNVLDDQLEEACKKYSEMGISGFKVDFLDRDDQTG
VEMVYRIAEMTAKYHLTLDLHGIYKPTGLNRTFPNIINFESVFGMEEMKWSTVEKDMMEY
DVTMPFIRMMCGPIDYTPGAMRNATKKDFQPIYYNPMSQGTRCHQLATYIVHDSPLTMLA
DNPTIYRQEQPCVDFICSVPNTGIDETRVLQGKMGEYIVTARRVGKNWYVGGLTDWNART
VKLDLSFLGEGNYEAVLFSDGVNAAKQAADYKKTEQTVERSSSLQIDLAPGGGFALSLIQ
K

Enzyme Prediction     

No EC number prediction in MGYG000002218_01924.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH97	19	658	3e-197	0.9857369255150554

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam10566	Glyco_hydro_97	9.14e-130	295	560	1	278	Glycoside hydrolase 97. This domain is the catalytic region of the bacterial glycosyl-hydrolase family 97. This central part of the GH97 family protein sequences represents a typical and complete (beta/alpha)8-barrel or catalytic TIM-barrel type domain. The N- and C-terminal parts of the sequences, mainly consisting of beta-strands, form two additional non-catalytic domains. In all known glycosidases with the (beta-alpha)8-barrel fold, the amino acid residues at the active site are located on the C-termini of the beta-strands.
pfam14508	GH97_N	8.14e-60	30	290	1	235	Glycosyl-hydrolase 97 N-terminal. This N-terminal domain of glycosyl-hydrolase-97 contributes part of the active site pocket. It is also important for contact with the catalytic and C-terminal domains of the whole.
pfam14509	GH97_C	1.79e-45	565	659	2	97	Glycosyl-hydrolase 97 C-terminal, oligomerization. Glycosyl-hydrolase-97 is made up of three tightly linked and highly conserved globular domains. The C-terminal domain is found to be necessary for oligomerization of the whole molecule in order to create the active-site pocket and the Ca++-binding site.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUU06805.1	3.51e-316	21	661	20	663
ALK83458.1	7.73e-282	3	658	5	661
ABR38815.1	6.58e-281	3	658	5	661
QQY38934.1	6.58e-281	3	658	5	661
QEW34704.1	1.87e-280	3	658	5	661

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3A24_A	1.14e-206	30	658	6	640	Crystalstructure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron],3A24_B Crystal structure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron]
5E1Q_A	2.08e-205	30	658	20	654	Mutant(D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482],5E1Q_B Mutant (D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482]
5HQC_A	2.03e-54	28	656	3	656	AGlycoside Hydrolase Family 97 enzyme R171K variant from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQ4_A	5.24e-54	28	656	3	656	AGlycoside Hydrolase Family 97 enzyme from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8],5HQA_A A Glycoside Hydrolase Family 97 enzyme in complex with Acarbose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQB_A	1.35e-53	28	656	3	656	AGlycoside Hydrolase Family 97 enzyme (E480Q) in complex with Panose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8A6L0	2.76e-207	7	658	4	661	Retaining alpha-galactosidase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_1871 PE=1 SV=1
D7CFN7	1.08e-43	29	655	44	617	Probable retaining alpha-galactosidase OS=Streptomyces bingchenggensis (strain BCW-1) OX=749414 GN=SBI_01652 PE=3 SV=1
G8JZS4	3.08e-39	1	658	1	723	Glucan 1,4-alpha-glucosidase SusB OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=susB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000229	0.999108	0.000168	0.000162	0.000153	0.000139

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002218_01924.