CAZyme Information: MGYG000002216_01132

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae
• CAZyme ID: MGYG000002216_01132
• CAZy Family: GH127
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2064	MGYG000002216_24|CGC1	228669.11	4.358

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002216	3003083	MAG	Spain	Europe

• Gene Location: Start: 4076; End: 10270 Strand: +

Full Sequence      

MKNKKVKKLLAFVMAVSIAGSSFTYVPARAAGLAGETVSTQAQGTSQTYLNNQGELKENP
LIQLPLGAVKAESWLENQLLLMKNGITGHMKDFAEYTAEGSAWLGSTNPGADTWERGPYF
VRGLTALAYALGDEELINETLDWLEWSVNSQQENGYFGPASDNSWWSRMPMLCAIRDFYE
AVEAKPVLERTQRERDLYGKVLNFFEKYFRYQANELPGRPLSNWADARGGDNLEVVYWLY
NRLYDAENPDATKWLLDLGDLLFRQTNDWTSIYNDTTVRQHVVNTSQGMKTPAVYSQYKD
DERYTTALSNGLDNMGIDHGRIDGLPNSDEAARENRSTRGSETCGIVEGILSTGLVMEIQ
GEPWMGDRMELLAYNSLPAAYPSDYSGHTYYVLQNQVMSTLGNHEFDCDHGDSSAFGAPC
GFDCCFANNHMGWAKFVQNMWMAKANGGLAIVAYGPNNVTATVADGKTAKFRQKTDYPFR
DTVQLDYYGDTAAFDLDLRIPEWATSATVTVNGVEQEGVQIGSYYTVNRTWEAGDQVQVT
FGSEVELTTWYNNSTAVQKGALIYGLQIEEDWRTYDENDARELKVEHHEDLPLREVYPAS
DWNYGLVTDENASFEVIESDEVGMQPFSSDAAPVKIIAKGVQLDEWTLDGNIAGPQPYGP
IDYDESEMVDITLVPYGSQRLRITHFPTMNNEMDTESVIRTESNQITRNGVTYQDFDNIV
VPKASDYCLKVTAEGSGTMIINSKYTQEVEGSFELGNLKSLLSGDFQFTSGHYNNIRFTG
DIRVSKVEVDVENREISDIQVLNVIRSGSTGKIITNLDAQETPYQVVYGTESGNYTHTVN
GFSSSTATLQDLEENTVYYAKVISTICGQEMESREVILEVAADQGGLKPNPNVPAATYEG
FSTLNYMTGEDGLWSTWGDTTIQAKTHENTTRQSEIKFGTSEEQKAILVAQGAQNWVDYV
VEADLTLDEVKGNNAGVMFRATNVGEGPDDYRGYFVGIGMVVNEPGMMVGCADGAWHDIQ
LIHGLDIKAGQKYTIKVVVYSDMFAVYLDDELMYVTQDSRFSNGTVGLRSYKVPFTGHDV
RVRSVTEEDLKVFEELVSSGEEPGEESGIHEDHKITEYEGFAQTAEETKLSFAFHDPEQK
IQVTDTEQGVQMNLGKGEKVKATLNTEGSEGWVDYVAEARLSVDVMDTNNCGIMFRATGV
GDKPDEYNGYFTGIGVAPEHEGSVLMVGYADGEWHDIEVIPWEIQPGQEYTLKVVAYDNQ
FAIYVDDVLAYRFTDDQFAAGTAGLRSYNEAFSVYDFTVRNVTAEDIKVFEEEEPEEPAV
IPDVTDELDSDAAWTKVGDTNLISVEEGWLKMGTSTNVKAVTGDESWSDMVYTADVKLED
GEGNAGLLVRSTREGSGADNYYGYYFGINGKQYEIGKSSNTWTQMKTGDLPLDTSQIHQL
KVVAYEDIFLFYVDGEMVAVIQDTDHEKGKVGIRGYNRSISLDRIEVRGLTEEEKEEVAH
QLELSKQMVISASSAEDSIQVKYPRMSNATSYRVLYGTESGVYTGEFTDIYFNGYKGSGI
FTHDKVAFTTPEPGTYYVKVLAMNGNAQVAYSNEVEVTTGERADTEKEAQALQDVMAQAE
KADLSGFTQVSLERVKQAKTNAQEVAGREQANQMQYETAADLLTVALNTPDSEVFGPEET
TVDKEDLQKLYDENLDKKEDDYTASTWEVFEEAMAAALEVLESDTADQKAVDDAYQVLSD
AVKGLKKKEELTQEDLAQAIQEANDAQAKAEAARDQALAAKAEADQAAQEAKAAKEKAEK
AMEDAKNMANAAEADKNAAIQAAEEAQARAEEAEKRAQEAQNALAAALEAQTKAEEAARL
AKEEVEAIRAMLKAALEEVDKAKAEAEKAKQEAEKAKQDALEAAKRAEEALAAYQNQQEQ
QKVKEGEIYQIGALKYQVTDTEKKTVSVVGTAKKKLTKVTIPATVKIKQEKYQVTSIAKK
AFWKQKKLQKVTIGKNVTTIGASAFYQSKNLKMVSFKTTKLKSVGKKAFAGIAKKAYLNV
PNKKAKAYKKLLQKAKAPKTAKIK

Enzyme Prediction     

No EC number prediction in MGYG000002216_01132.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	341	564	7.5e-25	0.42366412213740456
CBM66	1334	1480	1.4e-20	0.9290322580645162
CBM66	939	1071	2.8e-17	0.8

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	5.92e-29	124	564	64	502	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	8.82e-17	301	614	246	551	Uncharacterized conserved protein, DUF1680 family [Function unknown].
sd00036	LRR_3	8.57e-10	1954	2042	37	115	leucine-rich repeats. A leucine-rich repeat (LRR) is a structural protein motif of 20-30 amino acids that is unusually rich in the hydrophobic amino acid leucine. The conserved eleven-residue sequence motif (LxxLxLxxN/CxL) within the LRRs corresponds to the beta-strand and adjacent loop regions, whereas the remaining parts of the repeats are variable. LRRs fold together to form a solenoid protein domain, termed leucine-rich repeat domain. Leucine-rich repeats are usually involved in protein-protein interactions.
sd00036	LRR_3	2.89e-09	1954	2031	14	81	leucine-rich repeats. A leucine-rich repeat (LRR) is a structural protein motif of 20-30 amino acids that is unusually rich in the hydrophobic amino acid leucine. The conserved eleven-residue sequence motif (LxxLxLxxN/CxL) within the LRRs corresponds to the beta-strand and adjacent loop regions, whereas the remaining parts of the repeats are variable. LRRs fold together to form a solenoid protein domain, termed leucine-rich repeat domain. Leucine-rich repeats are usually involved in protein-protein interactions.
pfam13306	LRR_5	3.84e-09	1952	2031	9	77	Leucine rich repeats (6 copies). This family includes a number of leucine rich repeats. This family contains a large number of BSPA-like surface antigens from Trichomonas vaginalis.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNF30112.1	6.07e-130	52	744	31	740
QNK64903.1	1.25e-128	49	707	22	663
QPH39723.1	7.84e-128	52	696	29	656
QHS59113.1	2.44e-126	23	689	7	651
ALL07583.1	2.57e-126	49	689	28	651

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	7.65e-10	338	567	329	562	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	7.65e-10	462	567	448	562	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000433	0.998536	0.000276	0.000263	0.000240	0.000205

TMHMM  Annotations     

start	end
9	28