CAZyme Information: MGYG000002148_01541

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Eisenbergiella
• CAZyme ID: MGYG000002148_01541
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase BoGH2A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
797	MGYG000002148_10|CGC2	89506.04	5.8078

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002148	4597814	MAG	United States	North America

• Gene Location: Start: 25994; End: 28387 Strand: +

Full Sequence      

MKSLDFNENWTYKHLNSSEKPIPVTLPHDAMLYEERKESNPGGGNICWFAGGEYLYEKSF
FLSEDMAENHVEFDFEGVYRNAEVYINGEKAGGCAYGYTDFTVDGDRFLKYGEENRIQVA
VHNEKQPNSRWYTGSGIYRPVRMLTGPKEHIMADGVRIRTISINPAVVEVETRTTGSGMV
QVEILERETAVAKAEAQIQGEKAVCRMTVPKARLWEPGHPWLYTCRVSFQEDSAEERFGI
RMLEWDREKGLSINGNRVILRGACIHHDNGLLGACCYPDAEERKVGILMENGYNAIRSAH
NPCSRALLDACDRLGMLVMDEFVDCWYIHKTEYDYVNDFMDNWKFDLKRMVEKDYNHPSV
IMYSTGNEVSETGQKRGIELTGMMTRFLHAQDSTRPVTCGVNIFFNFLSSIGFGVYSDEK
AKKEAEKAEKDRTGAKKKKKVGSEFYNTMAGLFGDTTMKVGATLHGCDVKTRDAFANMDI
AGYNYGILRYAHDLKKYPQRLILGTETFCKDAYRFYEQAKENPGILGDFVWAGMDYLGEV
GIGSWEFEDYAPADPAVVGWLTAGSGRIDITGKPNGEAAYTKTVFGLTDRPVIAVKPVYQ
RGKHSPSAWKMTNAMESWSWRGCSGYPAEVEVYARAAEAELFVNGKSVGRRKFKKDCRVI
FQTAYEDGEVTAAVYDENGREISRSSLRTASPETRLAAVPEKEEIKAGELAYIRFSYTDE
TGIVKPMERHIISARVKGGRLLGMGSACPFQKESYLTPRADTYYGEALAIVQADGSGDVV
LEASDGEHECKAVVRVS

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	3	588	3.8e-101	0.5930851063829787

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	4.04e-55	4	417	28	447	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	1.73e-37	52	405	65	452	beta-D-glucuronidase; Provisional
PRK10340	ebgA	2.60e-23	55	368	112	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	8.12e-22	21	397	107	485	beta-galactosidase.
pfam02836	Glyco_hydro_2_C	1.33e-16	247	399	4	160	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRT30385.1	0.0	1	796	1	796
QHB24152.1	0.0	1	796	1	796
QEI31653.1	0.0	1	796	1	796
AUJ85302.1	0.0	1	797	1	784
AMG50431.1	0.0	1	797	1	784

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6B6L_A	1.09e-108	24	783	37	763	Thecrystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838],6B6L_B The crystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838]
7RSK_A	4.46e-108	24	783	37	763	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7RSK_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
7CWD_A	2.70e-85	1	784	1	788	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
5T98_A	1.14e-84	4	774	28	795	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
4YPJ_A	6.14e-82	2	784	8	794	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	7.16e-82	5	797	46	847	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	5.35e-57	5	696	50	714	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	7.65e-38	5	652	55	676	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	3.77e-27	4	396	6	411	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
P26257	5.40e-21	56	406	58	421	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000058	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002148_01541.