CAZyme Information: MGYG000002059_00726

Basic Information

• Species: Gemmiger sp900554145
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Gemmiger; Gemmiger sp900554145
• CAZyme ID: MGYG000002059_00726
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1148	MGYG000002059_37|CGC1	127329.36	4.9347

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002059	2037611	MAG	China	Asia

• Gene Location: Start: 8087; End: 11533 Strand: +

Full Sequence      

MKRTDFSVDWLFAACAPDTELLPWNAQFTPVDLPHDWQIRHADDLYRDGTLFYRKYFFVT
DVPGTRRMLWFGGAYMDTTVFVNGTEVGQWKYGYTSFWFDITDALHEGQNEVLVRCNLRH
PNSRWYSGAGLYRNVELWEAPDLHLMPDGLYVAAKEGDNGAWTVAVTAEAGSVTAASTGR
TLTLHLTDDNGSLLGTAAIPADAWAALPSPDGWRTGKETTIYKASHTFTLQDPALWDLDA
PSFYHITAELTGADGVTDTLTTRFGCRTIEATPDCGLLVNHKRVLIKGVCIHHDFGCLGS
AFEYSAAKRQLEILKSMGVNSIRTGHTPPAPQFMDLADEMGILMDVESFDCWRSGKNPYD
YGRFFDEWQEKDVAAWIRRDRNHPSLLFWCIGNEIYDTHAGPEGADTMRMLLAEVAAHDP
ARNGIPTLGSNYMPWENTQHCADIIKVAGYNYGERLYASHHEKHPDWVIYGSETSSIVQS
RGIYHFPLSQSLLSDDDLQCSSLGNSRTSWGAESWDICLQSEQRWPFTLGQYLWTGWDYI
GEPTPYHTRSSYFGTIDTAGFPKDAYYVVQAAWLDPKTHPMVHLFPYWDFNEGQLIDLCA
CTNAHSVELFVNGESLGRKVLDSAKSRTANWQTPYHPGSVKVVAYDENGKVVATDEQDSF
DDSAMVCLQADRKTISGDGRELAFITITTRDKNGNPVRNANDRVTVRVNGAGVLVGLDNG
DSADPDEYQTDSRRLFSGMLLAVVAGNGRTGTITVDVTAPGLRPAVLTLNAAPFEGPVRR
RLPPLTFGGSTQEIPVRKLTLTAERTALDKEHPVTHITAARRPAAATFTDIEWQLTDDKG
VPAVNAAMQPDGDVLTVTALGDGTLRVRALARNGHDAPQLISQLELSISGVGQLHKNPYE
FISASRFDASFGDIGNGNERGVSTSRTGRSWVLFDDIDFGPDGADTVELPIFVLDGEPTT
FRFWDGEPYAEGSTMIGERVYHKPKQWNVYQPDTFKLDKLLRGIGRFAVELNVKVHIKGF
TFTRHSRAWDTLAAGACDAVYGDSFTRDGSRVLGIGNNVSLLFDRMDFGETGCCGIRITG
RSPLPANTVHLMFAAADGGETERRVVEFGPQADWGEQTFTFEPVTGARQVTFLFLPGTQF
DFDSFTFI

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	3	578	3.7e-97	0.6143617021276596

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.21e-53	1	589	10	614	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam18565	Glyco_hydro2_C5	8.11e-36	668	768	3	103	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK10150	PRK10150	4.15e-32	47	422	61	443	beta-D-glucuronidase; Provisional
PRK10340	ebgA	1.73e-25	53	394	113	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam16355	DUF4982	4.61e-22	593	655	1	62	Domain of unknown function (DUF4982). This family is found in the C-terminal of uncharacterized proteins and beta-galactosidases around 680 residues in length from various Bacteroides species. The function of this protein is unknown.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCG34875.1	0.0	1	1148	1	1148
AYB44754.1	0.0	2	1148	3	1151
QOT12659.1	0.0	2	1148	3	1150
AGA58497.1	0.0	3	1147	4	1139
AET60078.1	0.0	6	1147	9	1139

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7CWD_A	3.64e-165	1	769	1	799	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	1.69e-154	2	762	8	798	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
5T98_A	3.21e-134	31	770	57	819	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
5DMY_A	2.01e-131	2	776	37	887	Beta-galactosidase- construct 33-930 [Bifidobacterium bifidum],5DMY_B Beta-galactosidase - construct 33-930 [Bifidobacterium bifidum],5DMY_C Beta-galactosidase - construct 33-930 [Bifidobacterium bifidum]
4CU6_A	2.63e-131	2	776	14	849	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU7_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU8_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	6.66e-144	2	771	43	848	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	7.28e-77	3	738	48	787	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	1.30e-44	3	644	53	702	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
T2KN75	1.86e-35	5	775	30	799	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1
P77989	1.87e-30	5	716	7	695	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.998615	0.001244	0.000169	0.000002	0.000001	0.000002

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002059_00726.